DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Nature 2014 imex curation Zhao H., Sheng G., Wang J., Wang M., Bunkoczi G., Gong W., Wei Z., Wang Y. Only protein-protein interactions ecolx Escherichia coli ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Zhao H., Sheng G., Wang J., Wang M., Bunkoczi G., Gong W., Wei Z., Wang Y. DIP original experiment: DIP-262311X Nature 2014 IMEx Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Nature 2014 imex curation Zhao H., Sheng G., Wang J., Wang M., Bunkoczi G., Gong W., Wei Z., Wang Y. Only protein-protein interactions ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhao H., Sheng G., Wang J., Wang M., Bunkoczi G., Gong W., Wei Z., Wang Y. DIP original experiment: DIP-262306X Nature 2014 IMEx Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Nature 2014 imex curation Zhao H., Sheng G., Wang J., Wang M., Bunkoczi G., Gong W., Wei Z., Wang Y. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhao H., Sheng G., Wang J., Wang M., Bunkoczi G., Gong W., Wei Z., Wang Y. DIP original experiment: DIP-262309X Nature 2014 IMEx Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Nature 2014 imex curation Zhao H., Sheng G., Wang J., Wang M., Bunkoczi G., Gong W., Wei Z., Wang Y. Only protein-protein interactions ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhao H., Sheng G., Wang J., Wang M., Bunkoczi G., Gong W., Wei Z., Wang Y. DIP original experiment: DIP-262307X Nature 2014 IMEx Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Nature 2014 imex curation Zhao H., Sheng G., Wang J., Wang M., Bunkoczi G., Gong W., Wei Z., Wang Y. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhao H., Sheng G., Wang J., Wang M., Bunkoczi G., Gong W., Wei Z., Wang Y. DIP original experiment: DIP-262308X Nature 2014 IMEx Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. Nature 2014 imex curation Zhao H., Sheng G., Wang J., Wang M., Bunkoczi G., Gong W., Wei Z., Wang Y. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhao H., Sheng G., Wang J., Wang M., Bunkoczi G., Gong W., Wei Z., Wang Y. DIP original experiment: DIP-262310X Nature 2014 IMEx Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. cas6_ecoli CRISPR system Cascade subunit CasE casE CasE endoRNase cas6e crRNA endonuclease ygcH JW2726 b2756 protein protein ecoli Escherichia coli (strain K12) MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKRNTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKRLDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQTVCFEGVLTINDAPALIDLVQQGIGPAKSMGCGLLSLAPL DIP protein Q46897 original sequence version: 61 DIP protein Q46897 original sequence version: 65 DIP protein Q46897 original sequence version: 94 DIP protein Q46897 original sequence version: 91 DIP protein Q46897 original sequence version: 101 1D5C060DD5FCAFAD cse2_ecoli CRISPR system Cascade subunit CasB casB cse2 ygcK JW2729 b2759 protein protein ecoli Escherichia coli (strain K12) MADEIDAMALYRAWQQLDNGSCAQIRRVSEPDELRDIPAFYRLVQPFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKSEQTTGISLGRALANSGRINERRIFQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA DIP protein P76632 original sequence version: 57 DIP protein P76632 original sequence version: 60 DIP protein P76632 original sequence version: 83 DIP protein P76632 original sequence version: 80 DIP protein P76632 original sequence version: 90 F2493AAD46595E5C cas5_ecoli CRISPR system Cascade subunit CasD casD cas5 ygcI b2757 JW5844 protein protein ecoli Escherichia coli (strain K12) MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACLGIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHETIQTWREYLCDASFTVALWLTPHATMVISELEKAVLKPRYTPYLGRRSCPLTHPLFLGTCQASDPQKALLNYEPVGGDIYSEESVTGHHLKFTARDEPMITLPRQFASREWYVIKGGMDVSQ DIP protein Q46898 original sequence version: 63 DIP protein Q46898 original sequence version: 66 DIP protein Q46898 original sequence version: 89 DIP protein Q46898 original sequence version: 86 DIP protein Q46898 original sequence version: 96 1C42CC009B317D68 cse1_ecoli CRISPR system Cascade subunit CasA casA cse1 ygcL JW2730 b2760 CRISPR type I-E/Ecoli-associated protein CasA/Cse1 CRISPR-associated protein CasA/Cse1 protein protein ecoli Escherichia coli (strain K12) MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDMELAALALLVCIGQIIAPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQTKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGGGFKSGLRGGTPVTTFVRGIDLRSTVLLNVLTLPRLQKQFPNESHTENQPTWIKPIKSNESIPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGLWPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQFRNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTALRKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANVNFSQADEVIADLRDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG DIP protein Q46901 original sequence version: 58 DIP protein Q46901 original sequence version: 61 DIP protein Q46901 original sequence version: 85 DIP protein Q46901 original sequence version: 82 DIP protein Q46901 original sequence version: 92 B3ACC755ABE8D965 casc_ecoli CRISPR system Cascade subunit CasC casC cas4 cse4 ygcJ JW2728 b2758 protein protein ecoli Escherichia coli (strain K12) MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSLKRAMRKSGYYAQNIGESSLRTIHLAQLRDVLRQKLGERFDQKIIDKTLALLSGKSVDEAEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDIALSGRMATSGMMTELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQEFSSGVFYRYANINLAQLQENLGGASREQALEIATHVVHMLATEVPGAKQRTYAAFNPADMVMVNFSDMPLSMANAFEKAVKAKDGFLQPSIQAFNQYWDRVANGYGLNGAAAQFSLSDVDPITAQVKQMPTLEQLKSWVRNNGEA DIP protein Q46899 original sequence version: 61 DIP protein Q46899 original sequence version: 64 DIP protein Q46899 original sequence version: 90 DIP protein Q46899 original sequence version: 86 DIP protein Q46899 original sequence version: 97 D9A99E0CBBD2B956 casb-casa-4 2 7 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role prey prey ecolx Escherichia coli 11 unspecified role unspecified role prey prey Q46899:p.[Phe200Ala;Asp204_Leu206delinsAlaAlaAla] F200A/D203A/D204A/L206A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain DDL AAA certain certain sequence position certain certain certain sequence position certain F A Wed May 02 14:30:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe200ala,asp_asp_leu204-206ala_ala_ala' Tue Jun 30 17:11:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe200Ala;Asp204_Leu206delinsAlaAlaAla]' ecolx Escherichia coli 8 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 10 unspecified role unspecified role prey prey ecolx Escherichia coli association association casa-casb-9 6 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association casa-casb-10 1 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association casa-casb-11 3 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role Region 75-104 Region 75-104 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association casa-casb-12 5 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Q46899:p.[Phe200Ala;Asp204_Leu206delinsAlaAlaAla] F200A/D203A/D204A/L206A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain DDL AAA certain certain sequence position certain certain certain sequence position certain F A Wed May 02 15:24:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe200ala,asp_asp_leu204-206ala_ala_ala' Tue Jun 30 16:50:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe200Ala;Asp204_Leu206delinsAlaAlaAla]' ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association casb-casa-5 4 7 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role prey prey Region 75-104 Region 75-104 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role prey prey ecolx Escherichia coli 8 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 10 unspecified role unspecified role prey prey ecolx Escherichia coli association association