DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone. Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone. Nature structural & molecular biology 2014 imex curation Yabukarski F., Lawrence P., Tarbouriech N., Bourhis JM., Delaforge E., Jensen MR., Ruigrok RW., Blackledge M., Volchkov V., Jamin M. Only protein-protein interactions in vitro In vitro light scattering light scattering predetermined predetermined participant predetermined Yabukarski F., Lawrence P., Tarbouriech N., Bourhis JM., Delaforge E., Jensen MR., Ruigrok RW., Blackledge M., Volchkov V., Jamin M. DIP original experiment: DIP-262112X Nature structural & molecular biology 2014 IMEx Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone. Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone. Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone. Nature structural & molecular biology 2014 imex curation Yabukarski F., Lawrence P., Tarbouriech N., Bourhis JM., Delaforge E., Jensen MR., Ruigrok RW., Blackledge M., Volchkov V., Jamin M. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Yabukarski F., Lawrence P., Tarbouriech N., Bourhis JM., Delaforge E., Jensen MR., Ruigrok RW., Blackledge M., Volchkov V., Jamin M. DIP original experiment: DIP-262113X Nature structural & molecular biology 2014 IMEx Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone. Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone. Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone. Nature structural & molecular biology 2014 imex curation Yabukarski F., Lawrence P., Tarbouriech N., Bourhis JM., Delaforge E., Jensen MR., Ruigrok RW., Blackledge M., Volchkov V., Jamin M. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yabukarski F., Lawrence P., Tarbouriech N., Bourhis JM., Delaforge E., Jensen MR., Ruigrok RW., Blackledge M., Volchkov V., Jamin M. DIP original experiment: DIP-262111X Nature structural & molecular biology 2014 IMEx Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone. Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone. Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone. Nature structural & molecular biology 2014 imex curation Yabukarski F., Lawrence P., Tarbouriech N., Bourhis JM., Delaforge E., Jensen MR., Ruigrok RW., Blackledge M., Volchkov V., Jamin M. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yabukarski F., Lawrence P., Tarbouriech N., Bourhis JM., Delaforge E., Jensen MR., Ruigrok RW., Blackledge M., Volchkov V., Jamin M. DIP original experiment: DIP-262114X Nature structural & molecular biology 2014 IMEx Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone. Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone. Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone. Nature structural & molecular biology 2014 imex curation Yabukarski F., Lawrence P., Tarbouriech N., Bourhis JM., Delaforge E., Jensen MR., Ruigrok RW., Blackledge M., Volchkov V., Jamin M. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Yabukarski F., Lawrence P., Tarbouriech N., Bourhis JM., Delaforge E., Jensen MR., Ruigrok RW., Blackledge M., Volchkov V., Jamin M. DIP original experiment: DIP-262115X Nature structural & molecular biology 2014 IMEx Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone. phosp_nipav Phosphoprotein P/V/C protein protein nipav Nipah virus MDKLELVNDGLNIIDFIQKNQKEIQKTYGRSSIQQPSIKDQTKAWEDFLQCTSGESEQVEGGMSKDDGDVERRNLEDLSSTSPTDGTIGKRVSNTRDWAEGSDDIQLDPVVTDVVYHDHGGECTGYGFTSSPERGWSDYTSGANNGNVCLVSDAKMLSYAPEIAVSKEDRETDLVHLENKLSTTGLNPTAVPFTLRNLSDPAKDSPVIAEHYYGLGVKEQNVGPQTSRNVNLDSIKLYTSDDEEADQLEFEDEFAGSSSEVIVGISPEDEEPSSVGGKPNESIGRTIEGQSIRDNLQAKDNKSTDVPGAGPKDSAVKEEPPQKRLPMLAEEFECSGSEDPIIRELLKENSLINCQQGKDAQPPYHWSIERSISPDKTEIVNGAVQTADRQRPGTPMPKSRGIPIKKGTDAKYPSAGTENVPGSKSGATRHVRGSPPYQEGKSVNAENVQLNASTAVKETDKSEVNPVDDNDSLDDKYIMPSDDFSNTFFPHDTDRLNYHADHLGDYDLETLCEESVLMGVINSIKLINLDMRLNHIEEQVKEIPKIINKLESIDRVLAKTNTALSTIEGHLVSMMIMIPGKGKGERKGKNNPELKPVIGRDILEQQSLFSFDNVKNFRDGSLTNEPYGAAVQLREDLILPELNFEETNASQFVPMADDSSRDVIKTLIRTHIKDRELRSELIGYLNKAENDEEIQEIANTVNDIIDGNI DIP protein Q9IK91 original sequence version: 60 ncap_nipav Nucleoprotein N Nucleocapsid protein protein protein nipav Nipah virus MSDIFEEAASFRSYQSKLGRDGRASAATATLTTKIRIFVPATNSPELRWELTLFALDVIRSPSAAESMKVGAAFTLISMYSERPGALIRSLLNDPDIEAVIIDVGSMVNGIPVMERRGDKAQEEMEGLMRILKTARDSSKGKTPFVDSRAYGLRITDMSTLVSAVITIEAQIWILIAKAVTAPDTAEESETRRWAKYVQQKRVNPFFALTQQWLTEMRNLLSQSLSVRKFMVEILIEVKKGGSAKGRAVEIISDIGNYVEETGMAGFFATIRFGLETRYPALALNEFQSDLNTIKSLMLLYREIGPRAPYMVLLEESIQTKFAPGGYPLLWSFAMGVATTIDRSMGALNINRGYLEPMYFRLGQKSARHHAGGIDQNMANRLGLSSDQVAELAAAVQETSAGRQESNVQAREAKFAAGGVLIGGSDQDIDEGEEPIEQSGRQSVTFKREMSISSLANSVPSSSVSTSGGTRLTNSLLNLRSRLAAKAAKEAASSNATDDPAISNRTQGESEKKNNQDLKPAQNDLDFVRADV DIP protein Q9IK92 original sequence version: 46 n-p/v/c-1 2 6 unspecified role unspecified role unspecified role unspecified role Region 1-50 Region 1-50 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 32-383 Region 32-383 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction n-p/v/c-2 3 6 unspecified role unspecified role unspecified role unspecified role Region 1-50 Region 1-50 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 32-383 Region 32-383 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction n-p/v/c-3 5 6 unspecified role unspecified role unspecified role unspecified role Region 1-100 Region 1-100 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 32-402 Region 32-402 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction n-p/v/c-4 4 6 unspecified role unspecified role unspecified role unspecified role Region 1-50 Region 1-50 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 32-383 Region 32-383 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction n-p/v/c-5 1 6 unspecified role unspecified role unspecified role unspecified role Region 1-50 Region 1-50 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 32-383 Region 32-383 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction