DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli. Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli. Science (New York, N.Y.) 2014 imex curation Jackson RN., Golden SM., van Erp PB., Carter J., Westra ER., Brouns SJ., van der Oost J., Terwilliger TC., Read RJ., Wiedenheft B. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Jackson RN., Golden SM., van Erp PB., Carter J., Westra ER., Brouns SJ., van der Oost J., Terwilliger TC., Read RJ., Wiedenheft B. DIP original experiment: DIP-264627X Science (New York, N.Y.) 2014 IMEx Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli. Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli. Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli. Science (New York, N.Y.) 2014 imex curation Jackson RN., Golden SM., van Erp PB., Carter J., Westra ER., Brouns SJ., van der Oost J., Terwilliger TC., Read RJ., Wiedenheft B. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Jackson RN., Golden SM., van Erp PB., Carter J., Westra ER., Brouns SJ., van der Oost J., Terwilliger TC., Read RJ., Wiedenheft B. DIP original experiment: DIP-264626X Science (New York, N.Y.) 2014 IMEx Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli. Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli. Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli. Science (New York, N.Y.) 2014 imex curation Jackson RN., Golden SM., van Erp PB., Carter J., Westra ER., Brouns SJ., van der Oost J., Terwilliger TC., Read RJ., Wiedenheft B. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jackson RN., Golden SM., van Erp PB., Carter J., Westra ER., Brouns SJ., van der Oost J., Terwilliger TC., Read RJ., Wiedenheft B. DIP original experiment: DIP-264625X Science (New York, N.Y.) 2014 IMEx Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli. cas6_ecoli CRISPR system Cascade subunit CasE casE CasE endoRNase cas6e crRNA endonuclease ygcH JW2726 b2756 protein protein ecoli Escherichia coli (strain K12) MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKRNTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKRLDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQTVCFEGVLTINDAPALIDLVQQGIGPAKSMGCGLLSLAPL DIP protein Q46897 original sequence version: 61 DIP protein Q46897 original sequence version: 65 DIP protein Q46897 original sequence version: 94 DIP protein Q46897 original sequence version: 91 DIP protein Q46897 original sequence version: 101 1D5C060DD5FCAFAD cse2_ecoli CRISPR system Cascade subunit CasB casB cse2 ygcK JW2729 b2759 protein protein ecoli Escherichia coli (strain K12) MADEIDAMALYRAWQQLDNGSCAQIRRVSEPDELRDIPAFYRLVQPFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKSEQTTGISLGRALANSGRINERRIFQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA DIP protein P76632 original sequence version: 57 DIP protein P76632 original sequence version: 60 DIP protein P76632 original sequence version: 83 DIP protein P76632 original sequence version: 80 DIP protein P76632 original sequence version: 90 F2493AAD46595E5C cas5_ecoli CRISPR system Cascade subunit CasD casD cas5 ygcI b2757 JW5844 protein protein ecoli Escherichia coli (strain K12) MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACLGIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHETIQTWREYLCDASFTVALWLTPHATMVISELEKAVLKPRYTPYLGRRSCPLTHPLFLGTCQASDPQKALLNYEPVGGDIYSEESVTGHHLKFTARDEPMITLPRQFASREWYVIKGGMDVSQ DIP protein Q46898 original sequence version: 63 DIP protein Q46898 original sequence version: 66 DIP protein Q46898 original sequence version: 89 DIP protein Q46898 original sequence version: 86 DIP protein Q46898 original sequence version: 96 1C42CC009B317D68 cse1_ecoli CRISPR system Cascade subunit CasA casA cse1 ygcL JW2730 b2760 CRISPR type I-E/Ecoli-associated protein CasA/Cse1 CRISPR-associated protein CasA/Cse1 protein protein ecoli Escherichia coli (strain K12) MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDMELAALALLVCIGQIIAPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQTKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGGGFKSGLRGGTPVTTFVRGIDLRSTVLLNVLTLPRLQKQFPNESHTENQPTWIKPIKSNESIPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGLWPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQFRNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTALRKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANVNFSQADEVIADLRDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG DIP protein Q46901 original sequence version: 58 DIP protein Q46901 original sequence version: 61 DIP protein Q46901 original sequence version: 85 DIP protein Q46901 original sequence version: 82 DIP protein Q46901 original sequence version: 92 B3ACC755ABE8D965 casc_ecoli CRISPR system Cascade subunit CasC casC cas4 cse4 ygcJ JW2728 b2758 protein protein ecoli Escherichia coli (strain K12) MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSLKRAMRKSGYYAQNIGESSLRTIHLAQLRDVLRQKLGERFDQKIIDKTLALLSGKSVDEAEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDIALSGRMATSGMMTELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQEFSSGVFYRYANINLAQLQENLGGASREQALEIATHVVHMLATEVPGAKQRTYAAFNPADMVMVNFSDMPLSMANAFEKAVKAKDGFLQPSIQAFNQYWDRVANGYGLNGAAAQFSLSDVDPITAQVKQMPTLEQLKSWVRNNGEA DIP protein Q46899 original sequence version: 61 DIP protein Q46899 original sequence version: 64 DIP protein Q46899 original sequence version: 90 DIP protein Q46899 original sequence version: 86 DIP protein Q46899 original sequence version: 97 D9A99E0CBBD2B956 casa-casb-7 3 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association casb-casa-3 1 4 unspecified role unspecified role prey prey ecolx Escherichia coli 6 unspecified role unspecified role prey prey ecolx Escherichia coli 8 unspecified role unspecified role prey prey ecolx Escherichia coli 5 unspecified role unspecified role bait bait StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 7 unspecified role unspecified role prey prey ecolx Escherichia coli association association casa-casb-8 2 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association