DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism. X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism. Science (New York, N.Y.) 2014 imex curation Chen L., Dürr KL., Gouaux E. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Chen L., Dürr KL., Gouaux E. DIP original experiment: DIP-266410X Science (New York, N.Y.) 2014 IMEx X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism. X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism. X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism. Science (New York, N.Y.) 2014 imex curation Chen L., Dürr KL., Gouaux E. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight silver stain molecular weight estimation by silver staining weight silver stain Chen L., Dürr KL., Gouaux E. DIP original experiment: DIP-266409X Science (New York, N.Y.) 2014 IMEx X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism. X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism. X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism. Science (New York, N.Y.) 2014 imex curation Chen L., Dürr KL., Gouaux E. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Chen L., Dürr KL., Gouaux E. DIP original experiment: DIP-266408X Science (New York, N.Y.) 2014 IMEx X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism. X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism. X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism. Science (New York, N.Y.) 2014 imex curation Chen L., Dürr KL., Gouaux E. Only protein-protein interactions ecolx Escherichia coli comigration in sds comigration in sds page comigration in sds weight by staining molecular weight estimation by staining weight by staining Chen L., Dürr KL., Gouaux E. DIP original experiment: DIP-266407X Science (New York, N.Y.) 2014 IMEx X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism. X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism. X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism. Science (New York, N.Y.) 2014 imex curation Chen L., Dürr KL., Gouaux E. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Chen L., Dürr KL., Gouaux E. DIP original experiment: DIP-266406X Science (New York, N.Y.) 2014 IMEx X-ray structures of AMPA receptor-cone snail toxin complexes illuminate activation mechanism. conii_const Con-ikot-ikot protein protein const Conus striatus Striated cone MAMNMSMTLCMFVMVVVAATVIDSTQLQEPDLSRMRRSGPADCCRMKECCTDRVNECLQRYSGREDKFVSFCYQEATVTCGSFNEIVGCCYGYQMCMIRVVKPNSLSGAHEACKTVSCGNPCA DIP protein P0CB20 original sequence version: 14 p19491-2 Glutamate receptor 2 Gria2 Glur2 AMPA-selective glutamate receptor 2 GluR-B GluR-K2 Glutamate receptor ionotropic, AMPA 2 protein protein rat Rattus norvegicus (Rat) Rat MQKIMHISVLLSPVLWGLIFGVSSNSIQIGGLFPRGADQEYSAFRVGMVQFSTSEFRLTPHIDNLEVANSFAVTNAFCSQFSRGVYAIFGFYDKKSVNTITSFCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLIEYYQWDKFAYLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDETYRSLFQDLELKKERRVILDCERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIERWSTLEEKEYPGAHTATIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANPAVPWGQGVEIERALKQVQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEVDKMVVTLTELPSGNDTSGLENKTVVVTTILESPYVMMKKNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVYGKADIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHTEEFEDGRETQSSESTNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLSKQTEIAYGTLDSGSTKEFFRRSKIAVFDKMWTYMRSAEPSVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSSLGTPVNLAVLKLSEQGVLDKLKNKWWYDKGECGAKDSGSKEKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKSRAEAKRMKVAKNPQNINPSSSQNSQNFATYKEGYNVYGIESVKI DIP protein P19491-2 original sequence version: 144 DIP protein P19491-2 original sequence version: 177 DIP protein P19491-2 original sequence version: 170 DIP protein P19491-2 original sequence version: 186 conii_const-1 5 6 unspecified role unspecified role unspecified role unspecified role Region 38-123 Region 38-123 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction conii_const-gria2-1 2 7 unspecified role unspecified role unspecified role unspecified role Region 25-850 Region 25-850 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 6 unspecified role unspecified role unspecified role unspecified role Region 38-123 Region 38-123 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction conii_const-2 4 6 unspecified role unspecified role unspecified role unspecified role Region 38-123 Region 38-123 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction conii_const-gria2-2 1 7 unspecified role unspecified role unspecified role unspecified role Region 25-850 Region 25-850 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 6 unspecified role unspecified role unspecified role unspecified role Region 38-123 Region 38-123 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction conii_const-3 3 6 unspecified role unspecified role unspecified role unspecified role Region 38-123 Region 38-123 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction