IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Identification of multifaceted binding modes for pyrin and ASC pyrin domains gives insights into pyrin inflammasome assembly. Identification of multifaceted binding modes for pyrin and ASC pyrin domains gives insights into pyrin inflammasome assembly. The Journal of biological chemistry 2014 imex curation Vajjhala PR., Kaiser S., Smith SJ., Ong QR., Soh SL., Stacey KJ., Hill JM., Vajjhala PR., Kaiser S., Smith SJ., Ong QR., Soh SL., Stacey KJ., Hill JM. dsp=84? mutations show Human origin Fig 1 - others?? Figure 7A: homodimerisation of ASC PYD not added as I do not see how an antibody to ASC can distinguish between GST-ASC and "soluble" ASC. The proteins ASC, and Pyrin were determined to be of Human origin based on mutation mapping and full length protein sequence length. p.vajjhala@uq.edu.au 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Vajjhala PR., Kaiser S., Smith SJ., Ong QR., Soh SL., Stacey KJ., Hill JM., Vajjhala PR., Kaiser S., Smith SJ., Ong QR., Soh SL., Stacey KJ., Hill JM. dsp=84? mutations show Human origin Fig 1 - others?? Figure 7A: homodimerisation of ASC PYD not added as I do not see how an antibody to ASC can distinguish between GST-ASC and "soluble" ASC. The proteins ASC, and Pyrin were determined to be of Human origin based on mutation mapping and full length protein sequence length. p.vajjhala@uq.edu.au Accepted 2017-MAY-26 AT 14:09 BST AT 14:09 BST by ORCHARD The Journal of biological chemistry 2014 imex curation I have removed No Uniprot update: added extra interaction and added "wt by Staining" to GST-ASC PYD molecule. Identification of multifaceted binding modes for pyrin and ASC pyrin domains gives insights into pyrin inflammasome assembly. Identification of multifaceted binding modes for pyrin and ASC pyrin domains gives insights into pyrin inflammasome assembly. The Journal of biological chemistry 2014 imex curation Vajjhala PR., Kaiser S., Smith SJ., Ong QR., Soh SL., Stacey KJ., Hill JM., Vajjhala PR., Kaiser S., Smith SJ., Ong QR., Soh SL., Stacey KJ., Hill JM. dsp=84? mutations show Human origin Fig 1 - others?? Figure 7A: homodimerisation of ASC PYD not added as I do not see how an antibody to ASC can distinguish between GST-ASC and "soluble" ASC. The proteins ASC, and Pyrin were determined to be of Human origin based on mutation mapping and full length protein sequence length. p.vajjhala@uq.edu.au 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Vajjhala PR., Kaiser S., Smith SJ., Ong QR., Soh SL., Stacey KJ., Hill JM., Vajjhala PR., Kaiser S., Smith SJ., Ong QR., Soh SL., Stacey KJ., Hill JM. dsp=84? mutations show Human origin Fig 1 - others?? Figure 7A: homodimerisation of ASC PYD not added as I do not see how an antibody to ASC can distinguish between GST-ASC and "soluble" ASC. The proteins ASC, and Pyrin were determined to be of Human origin based on mutation mapping and full length protein sequence length. p.vajjhala@uq.edu.au Accepted 2017-MAY-26 AT 14:09 BST AT 14:09 BST by ORCHARD The Journal of biological chemistry 2014 imex curation I have removed No Uniprot update: added extra interaction and added "wt by Staining" to GST-ASC PYD molecule. Identification of multifaceted binding modes for pyrin and ASC pyrin domains gives insights into pyrin inflammasome assembly. Identification of multifaceted binding modes for pyrin and ASC pyrin domains gives insights into pyrin inflammasome assembly. The Journal of biological chemistry 2014 imex curation Vajjhala PR., Kaiser S., Smith SJ., Ong QR., Soh SL., Stacey KJ., Hill JM., Vajjhala PR., Kaiser S., Smith SJ., Ong QR., Soh SL., Stacey KJ., Hill JM. dsp=84? mutations show Human origin Fig 1 - others?? Figure 7A: homodimerisation of ASC PYD not added as I do not see how an antibody to ASC can distinguish between GST-ASC and "soluble" ASC. The proteins ASC, and Pyrin were determined to be of Human origin based on mutation mapping and full length protein sequence length. p.vajjhala@uq.edu.au 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Vajjhala PR., Kaiser S., Smith SJ., Ong QR., Soh SL., Stacey KJ., Hill JM., Vajjhala PR., Kaiser S., Smith SJ., Ong QR., Soh SL., Stacey KJ., Hill JM. dsp=84? mutations show Human origin Fig 1 - others?? Figure 7A: homodimerisation of ASC PYD not added as I do not see how an antibody to ASC can distinguish between GST-ASC and "soluble" ASC. The proteins ASC, and Pyrin were determined to be of Human origin based on mutation mapping and full length protein sequence length. p.vajjhala@uq.edu.au Accepted 2017-MAY-26 AT 14:09 BST AT 14:09 BST by ORCHARD The Journal of biological chemistry 2014 imex curation I have removed No Uniprot update: added extra interaction and added "wt by Staining" to GST-ASC PYD molecule. Identification of multifaceted binding modes for pyrin and ASC pyrin domains gives insights into pyrin inflammasome assembly. Identification of multifaceted binding modes for pyrin and ASC pyrin domains gives insights into pyrin inflammasome assembly. The Journal of biological chemistry 2014 imex curation Vajjhala PR., Kaiser S., Smith SJ., Ong QR., Soh SL., Stacey KJ., Hill JM., Vajjhala PR., Kaiser S., Smith SJ., Ong QR., Soh SL., Stacey KJ., Hill JM. dsp=84? mutations show Human origin Fig 1 - others?? Figure 7A: homodimerisation of ASC PYD not added as I do not see how an antibody to ASC can distinguish between GST-ASC and "soluble" ASC. The proteins ASC, and Pyrin were determined to be of Human origin based on mutation mapping and full length protein sequence length. p.vajjhala@uq.edu.au 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Vajjhala PR., Kaiser S., Smith SJ., Ong QR., Soh SL., Stacey KJ., Hill JM., Vajjhala PR., Kaiser S., Smith SJ., Ong QR., Soh SL., Stacey KJ., Hill JM. dsp=84? mutations show Human origin Fig 1 - others?? Figure 7A: homodimerisation of ASC PYD not added as I do not see how an antibody to ASC can distinguish between GST-ASC and "soluble" ASC. The proteins ASC, and Pyrin were determined to be of Human origin based on mutation mapping and full length protein sequence length. p.vajjhala@uq.edu.au Accepted 2017-MAY-26 AT 14:09 BST AT 14:09 BST by ORCHARD The Journal of biological chemistry 2014 imex curation I have removed No Uniprot update: added extra interaction and added "wt by Staining" to GST-ASC PYD molecule. mefv_human Pyrin MEFV MEF Marenostrin TRIM20 protein protein human Homo sapiens Human MAKTPSDHLLSTLEELVPYDFEKFKFKLQNTSVQKEHSRIPRSQIQRARPVKMATLLVTYYGEEYAVQLTLQVLRAINQRLLAEELHRAAIQEYSTQENGTDDSAASSSLGENKPRSLKTPDHPEGNEGNGPRPYGGGAASLRCSQPEAGRGLSRKPLSKRREKASEGLDAQGKPRTRSPALPGGRSPGPCRALEGGQAEVRLRRNASSAGRLQGLAGGAPGQKECRPFEVYLPSGKMRPRSLEVTISTGEKAPANPEILLTLEEKTAANLDSATEPRARPTPDGGASADLKEGPGNPEHSVTGRPPDTAASPRCHAQEGDPVDGTCVRDSCSFPEAVSGHPQASGSRSPGCPRCQDSHERKSPGSLSPQPLPQCKRHLKQVQLLFCEDHDEPICLICSLSQEHQGHRVRPIEEVALEHKKKIQKQLEHLKKLRKSGEEQRSYGEEKAVSFLKQTEALKQRVQRKLEQVYYFLEQQEHFFVASLEDVGQMVGQIRKAYDTRVSQDIALLDALIGELEAKECQSEWELLQDIGDILHRAKTVPVPEKWTTPQEIKQKIQLLHQKSEFVEKSTKYFSETLRSEMEMFNVPELIGAQAHAVNVILDAETAYPNLIFSDDLKSVRLGNKWERLPDGPQRFDSCIIVLGSPSFLSGRRYWEVEVGDKTAWILGACKTSISRKGNMTLSPENGYWVVIMMKENEYQASSVPPTRLLIKEPPKRVGIFVDYRVGSISFYNVTARSHIYTFASCSFSGPLQPIFSPGTRDGGKNTAPLTICPVGGQGPD DIP protein O15553 original sequence version: 151 Defects in MEFV are the cause of familial Mediterranean fever autosomal recessive (ARFMF) [MIM:249100]. ARFMF is an inherited disorder characterized by recurrent episodic fever, serosal inflammation and pain in the abdomen, chest or joints. ARFMF is frequently complicated by amyloidosis, which leads to renal failure and can be prophylactically treated with colchicine. ARFMF primarily affects ancestral ethnic groups living around the Mediterranean basin: North African Jews, Armenians, Arabs and Turks. The disease is also distributed in other populations including Greeks, Cypriots, Italians and Spanish, although at a lower prevalence Probably controls the inflammatory response in myelomonocytic cells at the level of the cytoskeleton organization mint Defects in MEFV are the cause of familial Mediterranean fever autosomal dominant (ADFMF) [MIM:134610]. ADFMF is characterized by periodic fever, serosal inflammation and pain in the abdomen, chest or joints as seen also in the autosomal recessive form of the disease. It is associated with renal amyloidosis and characterized by colchicine unresponsiveness 3692E5E6E9FC8204 asc_human Apoptosis-associated speck-like protein containing a CARD PYD and CARD domain-containing protein Target of methylation-induced silencing 1 Caspase recruitment domain-containing protein 5 ASC CARD5 TMS1 PYCARD protein protein human Homo sapiens Human MGRARDAILDALENLTAEELKKFKLKLLSVPLREGYGRIPRGALLSMDALDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS DIP protein Q9ULZ3 original sequence version: 116 455987286586F46A pycard-mefv-1 4 5 unspecified role unspecified role prey prey c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range pyd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O15553:p.Lys3Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Wed May 02 18:43:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys3ala' Tue Jun 30 16:01:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys3Ala' O15553:p.Leu10Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Wed May 02 17:59:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu10ala' Tue Jun 30 18:45:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu10Ala' O15553:p.Gln29Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 15:36:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln29ala' Tue Jun 30 16:08:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln29Ala' O15553:p.Lys35Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Wed May 02 15:08:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys35ala' Tue Jun 30 18:04:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys35Ala' O15553:p.Gln46Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 16:25:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln46ala' Tue Jun 30 15:38:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln46Ala' O15553:p.Glu22Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain E A Tue May 09 10:45:39 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gly22ala' Wed May 02 15:10:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu22ala' Tue Jun 30 18:43:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu22Ala' O15553:p.Lys25Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:26:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys25ala' Tue Jun 30 17:12:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys25Ala' O15553:p.Val58Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain V A Wed May 02 16:14:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val58ala' Tue Jun 30 18:38:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val58Ala' O15553:p.Glu63Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 16:44:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu63ala' Tue Jun 30 18:29:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu63Ala' O15553:p.His37Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H A Wed May 02 17:53:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his37ala' Tue Jun 30 16:44:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His37Ala' O15553:p.Arg42Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:02:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg42ala' Tue Jun 30 19:01:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg42Ala' O15553:p.Arg49Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:40:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg49ala' Tue Jun 30 19:11:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg49Ala' O15553:p.Leu71Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Wed May 02 14:57:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu71ala' Tue Jun 30 17:22:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu71Ala' O15553:p.Arg80Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 14:38:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg80ala' Tue Jun 30 19:04:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg80Ala' O15553:p.Glu84Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 16:28:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu84ala' Tue Jun 30 20:09:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu84Ala' O15553:p.Arg88Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:42:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg88ala' Tue Jun 30 19:49:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg88Ala' O15553:p.Glu14Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 17:04:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu14ala' Tue Jun 30 19:09:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu14Ala' O15553:p.Arg75Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 17:08:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg75ala' Tue Jun 30 16:43:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg75Ala' O15553:p.Arg42Trp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R W dbSNP:rs61754767 Wed May 02 15:01:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg42trp' Tue Jun 30 15:44:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg42Trp' O15553:p.Ala89Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A T Wed May 02 18:15:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala89thr' Tue Jun 30 20:14:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala89Thr' ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait pyd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 32 10 direct interaction direct interaction 1D, 9A-C mutations decreasing/increasing taken from text as is not obvious from figure. pycard-mefv-2 4 5 unspecified role unspecified role prey prey c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range pyd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait Q9ULZ3:p.Asp10Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 15:30:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp10ala' Tue Jun 30 18:45:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp10Ala' Q9ULZ3:p.Glu13Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 16:37:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu13ala' Tue Jun 30 16:13:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu13Ala' Q9ULZ3:p.Glu18Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 15:23:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu18ala' Tue Jun 30 19:42:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu18Ala' Q9ULZ3:p.Lys21Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:45:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys21ala' Tue Jun 30 18:27:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys21Ala' Q9ULZ3:p.Glu34Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 16:18:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu34ala' Tue Jun 30 19:15:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu34Ala' Q9ULZ3:p.Arg38Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 17:05:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg38ala' Tue Jun 30 17:17:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg38Ala' Q9ULZ3:p.Arg41Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:37:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg41ala' Tue Jun 30 19:53:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg41Ala' Q9ULZ3:p.Asp48Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 18:28:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp48ala' Tue Jun 30 17:51:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp48Ala' Q9ULZ3:p.Arg74Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:17:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg74ala' Tue Jun 30 18:06:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg74Ala' Q9ULZ3:p.Glu80Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 14:34:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu80ala' Tue Jun 30 20:23:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu80Ala' Q9ULZ3:p.Asp54Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 17:51:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp54ala' Tue Jun 30 20:05:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp54Ala' Q9ULZ3:p.Thr63Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T A Wed May 02 14:50:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr63ala' Tue Jun 30 16:12:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr63Ala' Q9ULZ3:p.Asp51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 18:54:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp51ala' Tue Jun 30 19:20:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp51Ala' Q9ULZ3:p.Glu67Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 16:12:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu67ala' Tue Jun 30 16:01:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu67Ala' pyd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9ULZ3:p.Arg3Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:15:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg3ala' Tue Jun 30 16:42:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg3Ala' Q9ULZ3:p.Gln84Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 19:51:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln84ala' Tue Jun 30 17:50:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln84Ala' ecoli Escherichia coli (strain K12) 53 37 direct interaction direct interaction 5A-C mutations decreasing/increasing taken from text as is not obvious from figure. pycard-mefv-4 1 5 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 6 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range pyd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 stimulator stimulator prey prey pyd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 7B pycard-mefv-3 2 5 unspecified role unspecified role prey prey c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range pyd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range pyd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 68 71 physical association physical association 7Ai Soluble Pyrin PYD was present;it is stated that pyrin PYD inhibited ASC PYD self-association. mefv-mefv-1 1 5 unspecified role unspecified role prey prey delta_N sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range in vitro In vitro 5 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range pyd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O15553:p.Arg42Trp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R W Wed May 02 18:57:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg42trp' Tue Jun 30 19:02:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg42Trp' O15553:p.Ala89Thr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain A T Wed May 02 19:23:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala89thr' Tue Jun 30 16:02:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala89Thr' ecoli Escherichia coli (strain K12) 74 79 direct interaction direct interaction 9D mefv-pycard-3 3 5 unspecified role unspecified role bait bait c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range O15553:p.Arg42Trp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R W Wed May 02 20:01:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg42trp' Tue Jun 30 19:20:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg42Trp' O15553:p.Ala89Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A T Wed May 02 17:52:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala89thr' Tue Jun 30 20:23:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala89Thr' ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey in vitro In vitro physical association physical association 9 E/F Binding of ASC to the R42W and A89T pyrin mutants is similar to that of wild-type, in the presence or absence of PSTPIP1. pycard-mefv-5 2 5 competitor competitor competitor prey prey c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range pyd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 weight by staining molecular weight estimation by staining weight by staining anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range pyd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey pyd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 7A Soluble Pyrin PYD was present;it is stated that pyrin PYD inhibited ASC PYD self-association.