DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structure (London, England : 1993) 2014 imex curation Moharana K., Zabeau L., Peelman F., Ringler P., Stahlberg H., Tavernier J., Savvides SN. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Moharana K., Zabeau L., Peelman F., Ringler P., Stahlberg H., Tavernier J., Savvides SN. DIP original experiment: DIP-261928X Structure (London, England : 1993) 2014 IMEx Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structure (London, England : 1993) 2014 imex curation Moharana K., Zabeau L., Peelman F., Ringler P., Stahlberg H., Tavernier J., Savvides SN. Only protein-protein interactions in vitro In vitro field flow fractionation field flow fractionation predetermined predetermined participant predetermined Moharana K., Zabeau L., Peelman F., Ringler P., Stahlberg H., Tavernier J., Savvides SN. DIP original experiment: DIP-261930X Structure (London, England : 1993) 2014 IMEx Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structure (London, England : 1993) 2014 imex curation Moharana K., Zabeau L., Peelman F., Ringler P., Stahlberg H., Tavernier J., Savvides SN. Only protein-protein interactions in vitro In vitro sls static light scattering sls predetermined predetermined participant predetermined Moharana K., Zabeau L., Peelman F., Ringler P., Stahlberg H., Tavernier J., Savvides SN. DIP original experiment: DIP-261931X Structure (London, England : 1993) 2014 IMEx Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structure (London, England : 1993) 2014 imex curation Moharana K., Zabeau L., Peelman F., Ringler P., Stahlberg H., Tavernier J., Savvides SN. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Moharana K., Zabeau L., Peelman F., Ringler P., Stahlberg H., Tavernier J., Savvides SN. DIP original experiment: DIP-261933X Structure (London, England : 1993) 2014 IMEx Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structure (London, England : 1993) 2014 imex curation Moharana K., Zabeau L., Peelman F., Ringler P., Stahlberg H., Tavernier J., Savvides SN. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Moharana K., Zabeau L., Peelman F., Ringler P., Stahlberg H., Tavernier J., Savvides SN. DIP original experiment: DIP-261932X Structure (London, England : 1993) 2014 IMEx Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. Structure (London, England : 1993) 2014 imex curation Moharana K., Zabeau L., Peelman F., Ringler P., Stahlberg H., Tavernier J., Savvides SN. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Moharana K., Zabeau L., Peelman F., Ringler P., Stahlberg H., Tavernier J., Savvides SN. DIP original experiment: DIP-261929X Structure (London, England : 1993) 2014 IMEx Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins. lepr_mouse Leptin receptor Lepr Db Obr OB receptor B219 protein protein mouse Mus musculus Mouse MMCQKFYVVLLHWEFLYVIAALNLAYPISPWKFKLFCGPPNTTDDSFLSPAGAPNNASALKGASEAIVEAKFNSSGIYVPELSKTVFHCCFGNEQGQNCSALTDNTEGKTLASVVKASVFRQLGVNWDIECWMKGDLTLFICHMEPLPKNPFKNYDSKVHLLYDLPEVIDDSPLPPLKDSFQTVQCNCSLRGCECHVPVPRAKLNYALLMYLEITSAGVSFQSPLMSLQPMLVVKPDPPLGLHMEVTDDGNLKISWDSQTMAPFPLQYQVKYLENSTIVREAAEIVSATSLLVDSVLPGSSYEVQVRSKRLDGSGVWSDWSSPQVFTTQDVVYFPPKILTSVGSNASFHCIYKNENQIISSKQIVWWRNLAEKIPEIQYSIVSDRVSKVTFSNLKATRPRGKFTYDAVYCCNEQACHHRYAELYVIDVNINISCETDGYLTKMTCRWSPSTIQSLVGSTVQLRYHRRSLYCPDSPSIHPTSEPKNCVLQRDGFYECVFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSNVKAEITVNTGLLKVSWEKPVFPENNLQFQIRYGLSGKEIQWKTHEVFDAKSKSASLLVSDLCAVYVVQVRCRRLDGLGYWSNWSSPAYTLVMDVKVPMRGPEFWRKMDGDVTKKERNVTLLWKPLTKNDSLCSVRRYVVKHRTAHNGTWSEDVGNRTNLTFLWTEPAHTVTVLAVNSLGASLVNFNLTFSWPMSKVSAVESLSAYPLSSSCVILSWTLSPDDYSLLYLVIEWKILNEDDGMKWLRIPSNVKKFYIHDNFIPIEKYQFSLYPVFMEGVGKPKIINGFTKDAIDKQQNDAGLYVIVPIIISSCVLLLGTLLISHQRMKKLFWDDVPNPKNCSWAQGLNFQKPETFEHLFTKHAESVIFGPLLLEPEPISEEISVDTAWKNKDEMVPAAMVSLLLTTPDPESSSICISDQCNSANFSGSQSTQVTCEDECQRQPSVKYATLVSNDKLVETDEEQGFIHSPVSNCISSNHSPLRQSFSSSSWETEAQTFFLLSDQQPTMISPQLSFSGLDELLELEGSFPEENHREKSVCYLGVTSVNRRESGVLLTGEAGILCTFPAQCLFSDIRILQERCSHFVENNLSLGTSGENFVPYMPQFQTCSTHSHKIMENKMCDLTV DIP protein P48356 original sequence version: 111 DIP protein P48356 original sequence version: 144 0E1E75B076BA60A2 lep_mouse Leptin Lep Ob Obesity factor protein protein mouse Mus musculus Mouse MCWRPLCRFLWLWSYLSYVQAVPIQKVQDDTKTLIKTIVTRINDISHTQSVSAKQRVTGLDFIPGLHPILSLSKMDQTLAVYQQVLTSLPSQNVLQIANDLENLRDLLHLLAFSKSCSLPQTSGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDVSPEC DIP protein P41160 original sequence version: 123 lep-lepr-1 2 7 unspecified role unspecified role unspecified role unspecified role Region 22-839 Region 22-839 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 8 unspecified role unspecified role unspecified role unspecified role Region 22-167 Region 22-167 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction lep-lepr-2 1 7 unspecified role unspecified role unspecified role unspecified role Region 22-839 Region 22-839 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 8 unspecified role unspecified role unspecified role unspecified role Region 22-167 Region 22-167 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction lep-lepr-3 5 7 unspecified role unspecified role unspecified role unspecified role Region 22-839 Region 22-839 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 8 unspecified role unspecified role unspecified role unspecified role Region 22-167 Region 22-167 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction lep-lepr-4 3 7 unspecified role unspecified role unspecified role unspecified role Region 22-839 Region 22-839 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 8 unspecified role unspecified role unspecified role unspecified role Region 22-167 Region 22-167 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction lep-lepr-5 6 7 unspecified role unspecified role unspecified role unspecified role Region 22-839 Region 22-839 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 8 unspecified role unspecified role unspecified role unspecified role Region 22-167 Region 22-167 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction lep-lepr-6 4 7 unspecified role unspecified role unspecified role unspecified role Region 22-839 Region 22-839 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 8 unspecified role unspecified role unspecified role unspecified role Region 22-167 Region 22-167 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction