DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of the AcrAB-TolC multidrug efflux pump. Structure of the AcrAB-TolC multidrug efflux pump. Nature 2014 imex curation Du D., Wang Z., James NR., Voss JE., Klimont E., Ohene-Agyei T., Venter H., Chiu W., Luisi BF. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Du D., Wang Z., James NR., Voss JE., Klimont E., Ohene-Agyei T., Venter H., Chiu W., Luisi BF. DIP original experiment: DIP-260849X Nature 2014 IMEx Structure of the AcrAB-TolC multidrug efflux pump. Structure of the AcrAB-TolC multidrug efflux pump. Structure of the AcrAB-TolC multidrug efflux pump. Nature 2014 imex curation Du D., Wang Z., James NR., Voss JE., Klimont E., Ohene-Agyei T., Venter H., Chiu W., Luisi BF. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Du D., Wang Z., James NR., Voss JE., Klimont E., Ohene-Agyei T., Venter H., Chiu W., Luisi BF. DIP original experiment: DIP-260850X Nature 2014 IMEx Structure of the AcrAB-TolC multidrug efflux pump. Structure of the AcrAB-TolC multidrug efflux pump. Structure of the AcrAB-TolC multidrug efflux pump. Nature 2014 imex curation Du D., Wang Z., James NR., Voss JE., Klimont E., Ohene-Agyei T., Venter H., Chiu W., Luisi BF. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Du D., Wang Z., James NR., Voss JE., Klimont E., Ohene-Agyei T., Venter H., Chiu W., Luisi BF. DIP original experiment: DIP-260851X Nature 2014 IMEx Structure of the AcrAB-TolC multidrug efflux pump. acrb_ecoli Multidrug efflux pump subunit AcrB acrB acrE b0462 JW0451 AcrAB-TolC multidrug efflux pump subunit AcrB Acridine resistance protein B protein protein ecoli Escherichia coli (strain K12) MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVTISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADIAQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAANMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQLGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYDIIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVKISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINTLTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLSAVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEGKKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPDEDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNTGIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDFELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALGVSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQMVPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVGYDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALLAATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRLRPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVRRRFSRKNEDIEHSHTVDHH DIP protein P31224 original sequence version: 128 DIP protein P31224 original sequence version: 73 DIP protein P31224 original sequence version: 136 DIP protein P31224 original sequence version: 112 DIP protein P31224 original sequence version: 126 DIP protein P31224 original sequence version: null 19670E3C4CC29055 acrz_ecoli Multidrug efflux pump accessory protein AcrZ acrZ AcrAB-TolC multidrug efflux pump accessory protein AcrZ Acridine resistance protein Z ybhT JW5102 b0762 protein protein ecoli Escherichia coli (strain K12) MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQPGQNH DIP protein P0AAW9 original sequence version: 58 1B1185D27F1F37F7 acrb-acrz-1 1 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction acrb-acrz-2 2 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction acrz-acrb-1 3 5 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 4 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association