DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Nature cell biology 2014 imex curation Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. DIP original experiment: DIP-260968X Nature cell biology 2014 IMEx Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Nature cell biology 2014 imex curation Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. DIP original experiment: DIP-260964X Nature cell biology 2014 IMEx Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Nature cell biology 2014 imex curation Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. DIP original experiment: DIP-260963X Nature cell biology 2014 IMEx Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Nature cell biology 2014 imex curation Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. DIP original experiment: DIP-260962X Nature cell biology 2014 IMEx Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Nature cell biology 2014 imex curation Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. DIP original experiment: DIP-260966X Nature cell biology 2014 IMEx Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Nature cell biology 2014 imex curation Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. DIP original experiment: DIP-260961X Nature cell biology 2014 IMEx Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Nature cell biology 2014 imex curation Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. DIP original experiment: DIP-260967X Nature cell biology 2014 IMEx Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Nature cell biology 2014 imex curation Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. DIP original experiment: DIP-260965X Nature cell biology 2014 IMEx Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Nature cell biology 2014 imex curation Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. DIP original experiment: DIP-260960X Nature cell biology 2014 IMEx Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Nature cell biology 2014 imex curation Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. DIP original experiment: DIP-260969X Nature cell biology 2014 IMEx Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. Nature cell biology 2014 imex curation Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Sawa-Makarska J., Abert C., Romanov J., Zens B., Ibiricu I., Martens S. DIP original experiment: DIP-260959X Nature cell biology 2014 IMEx Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane-cargo apposition during selective autophagy. atg11_yeast Autophagy-related protein 11 Cytoplasm to vacuole targeting protein 9 ATG11 CVT9 YPR049C YP9499.07c protein protein yeast Saccharomyces cerevisiae Baker's yeast MADADEYSTAPTQQEITPLQTTATIINAISGECITTNVDFFVSLDKFKQFIARKWKIPPDQLLILLPYGNKLKPSMFKELLINRSFTLNDFYVYDRRLFSLVSKPTPTNLLTSKDSNPMNSPNSNDLTETLEYLIKNSHISQYQGSDTIMIKPMPSPLEDADVDLSRLNYHSVTSLLTTNLGWLSALEIDVHYFKSLIPDIIAHIKRIFDGLTVCSQYLKLYCFDVESLYNSNVQFLNQLVDNGMTSKWEKCFNDTLSKLTALEGDSLQKFINIESLLENEKSVKILNHSINGKLNKIKREIDENASFRDIITVNIDRLRQMFTPNESKFELEDQMAESFEVLVSEMRTRSRNVLDKEEEEFNSQEFLKSMNVMLEKDKKESVKTLFTISQALYSQIGELIDLKKSLQKHAVAILGNIAFTQMEILGIKRLLLNECNKDLELYKKYEVEFAQVEDLPLIYGLYLIEKYRRLSWFQQILSFISNFNQDLELFKQNELRTRNKWVKNFGSIATVFCEDLLSSSDFKRLNEYHSHTSPPNEDEEDENENSIANYRQDLVKVSQAIDNYMTQIKETDVSEPIIDLLSKTLFETKRFHIIYSNFKNNNNNSSNGNSISPEGSIALKSDDVVKGYKTRIKKLESLLHEFQYSDIGHWPQGVLNTHLKPFRGSATSINKKKFLGASVLLEPANISEVNIDSVSQANNHQIQELESNVDDLLHQLQLLKEENNRKSMQISEMGKKISDLEVEKTAYRETLTNLNQELARLTNEEQSHRTEIFTLNASFKKQLNDIISQDNEKIEKLTGDYDDVSKSRERLQMDLDESNKKHEQEVNLLKADIERLGKQIVTSEKSYAETNSSSMEKGEKFETIPLAEDPGRENQISAYTQTLQDRIFDIISTNIFILENIGLLLTFDNNNNIQIRRVKGLKKGTAQSNILDESTQMLDAHDNSLIKSPVFQKLKDEYELIKSVANGSEKDTQQSIFLGNITQLYDNKLYEVAVIRRFKDIETLAKKLTKENKIKRTLLERFQREKVTLRNFQIGDLALFLPTRENVNSVGSMSSSTSSLSSSFSSVDLSTPPPLDAMSIQSSPSVIHSNVINQASISGRDKNKLMRPWAAFTAFEESTRYFLKDEKGLTKGKEWFVGRIVTLEHFVADSPSNNPFRLPKGSVWFQVTAVVVSYQGV A904B92E9AE79449 A904B92E9AE79449 ampl_yeast Vacuolar aminopeptidase 1 APE1 API Aminopeptidase yscI LAP4 Leucine aminopeptidase IV Lysosomal aminopeptidase III Polypeptidase Vacuolar aminopeptidase I YSC1 YKL455 YKL103C protein protein yeast Saccharomyces cerevisiae Baker's yeast MEEQREILEQLKKTLQMLTVEPSKNNQIANEEKEKKENENSWCILEHNYEDIAQEFIDFIYKNPTTYHVVSFFAELLDKHNFKYLSEKSNWQDSIGEDGGKFYTIRNGTNLSAFILGKNWRAEKGVGVIGSHVDALTVKLKPVSFKDTAEGYGRIAVAPYGGTLNELWLDRDLGIGGRLLYKKKGTNEIKSALVDSTPLPVCRIPSLAPHFGKPAEGPFDKEDQTIPVIGFPTPDEEGNEPPTDDEKKSPLFGKHCIHLLRYVAKLAGVEVSELIQMDLDLFDVQKGTIGGIGKHFLFAPRLDDRLCSFAAMIALICYAKDVNTEESDLFSTVTLYDNEEIGSLTRQGAKGGLLESVVERSSSAFTKKPVDLHTVWANSIILSADVNHLYNPNFPEVYLKNHFPVPNVGITLSLDPNGHMATDVVGTALVEELARRNGDKVQYFQIKNNSRSGGTIGPSLASQTGARTIDLGIAQLSMHSIRAATGSKDVGLGVKFFNGFFKHWRSVYDEFGEL 702A8C88A2124C24 702A8C88A2124C24 atg34_yeast Autophagy-related protein 34 ATG34 O0957 YOL083W ATG19-B protein protein yeast Saccharomyces cerevisiae Baker's yeast MKIAVETTLFDFFVIDQFKKSTFSAPNTKVDTIKGCINKFIEQFNVYDEQHIFWQPPGKSNVRLLSNANDFGQLGNFLHKKIKCNIFIGEEALRKYDLNICGPYDKFVENSDPSVKKVVNRDDVMLSRKCLNIISEQLSILEKSISKAQNQVLQSSEVEGKKCIILPEDKPELIKFFSKFETSVQLQEVYEGYKVYEKLLQKFGGQKKRMESFLNENTPMSGAEAIKQINISEELKEKGERLTTPNDPLLHVEVSNEDNSLHFILYNKTNIIIPGNCTFEFSSQISEVFSIKMGPHEIGIKGQKELWFFPSLPTPLSNYTMKVVNQDGETILVGKCADSNEITLKSPLASFSTGSFQTGSFHTLQDPTNVFRADALSSPDESSIMSTPFLGETDEVYNSGSTLSRPFTWEEI 5F339CC6AC2824D5 5F339CC6AC2824D5 atg8_yeast Autophagy-related protein 8 ATG8 APG8 AUT7 YBL078C YBL0732 CVT5 Autophagy-related ubiquitin-like modifier ATG8 Cytoplasm to vacuole targeting protein 5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKSTFKSEYPFEKRKAESERIADRFKNRIPVICEKAEKSDIPEIDKRKYLVPADLTVGQFVYVIRKRIMLPPEKAIFIFVNDTLPPTAALMSAIYQEHKDKDGFLYVTYSGENTFGR 1246CEFF5A038819 1246CEFF5A038819 atg19_yeast Autophagy-related protein 19 Cytoplasm-to-vacuole targeting protein 19 ATG19 CVT19 YOL082W O0980 YOL01 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNNSKTNQQMNTSMGYPLTVYDECNKFQLIVPTLDANIMLWCIGQLSLLNDSNGCKHLFWQPNDKSNVRILLNNYDYGHLFKYLQCQRKCSVYIGEGTLKKYNLTISTSFDNFLDLTPSEEKESLCREDAHEDPVSPKAGSEEEISPNSTSNVVVSRECLDNFMKQLLKLEESLNKLELEQKVTNKEPNHRISGTIDIPEDRSELVNFFTELKTVKQLEDVFQRYHDYERLSQECDSKTEIASDHSKKETKIEVEPPNERSLQITMNQRDNSLYFQLFNNTNSVLAGNCKLKFTDAGDKPTTQIIDMGPHEIGIKEYKEYRYFPYALDLEAGSTIEIENQYGEVIFLGKYGSSPMINLRPPSRLSAESLQASQEPFYSFQIDTLPELDDSSIISTSISLSYDGDDNEKALTWEEL B49DA1DF83A04070 B49DA1DF83A04070 atg19-atg8-1 6 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction atg19-atg8-2 11 16 unspecified role unspecified role unspecified role unspecified role Region 365-415 Region 365-415 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P35193:p.Trp412Ala W412A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W412A'. Wed May 02 16:33:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp412ala' Tue Jun 30 17:03:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp412Ala' ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction Deletion of region 408-415 decreased the interaction rate/strength. atg34-atg8-1 2 14 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 15 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction atg8-atg19-1 10 16 unspecified role unspecified role prey prey P35193:p.[Phe376Ala;Phe379Ala;Pro385Ala;Glu386Ala;Trp412Ala] F376A/F379A/P385A/E386A/W412A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F376A/F379A/P385A/E386A/W412A'. Wed May 02 16:01:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe376ala,phe379ala,pro385ala,glu386ala,trp412ala' Tue Jun 30 20:14:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe376Ala;Phe379Ala;Pro385Ala;Glu386Ala;Trp412Ala]' P35193:p.[Phe376Ala;Phe379Ala;Trp412Ala] F376A/F379A/W412A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F376A/F379A/W412A'. Wed May 02 16:55:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe376ala,phe379ala,trp412ala' Tue Jun 30 17:44:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe376Ala;Phe379Ala;Trp412Ala]' P35193:p.[Phe376Ala;Phe379Ala] F376A/F379A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F376A/F379A'. Wed May 02 20:26:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe376ala,phe379ala' Tue Jun 30 17:22:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe376Ala;Phe379Ala]' Region 1-384 Region 1-384 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction ape1-atg8-1 7 15 unspecified role unspecified role prey prey ecolx Escherichia coli 13 unspecified role unspecified role bait bait Region 1-45 Region 1-45 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association atg11-atg34-1 4 14 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 12 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association atg19-atg8-3 8 16 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 376-415 Region 376-415 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P35193:p.Trp412Ala W412A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W412A'. Wed May 02 15:45:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp412ala' Tue Jun 30 17:02:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp412Ala' Region 1-384 Region 1-384 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P35193:p.[Phe376Ala;Phe379Ala;Pro385Ala;Glu386Ala;Trp412Ala] F376A/F379A/P385A/E386A/W412A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F376A/F379A/P385A/E386A/W412A'. Wed May 02 19:30:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe376ala,phe379ala,pro385ala,glu386ala,trp412ala' Tue Jun 30 16:27:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe376Ala;Phe379Ala;Pro385Ala;Glu386Ala;Trp412Ala]' P35193:p.[Pro385Ala;Glu386Ala;Trp412Ala] P385A/E386A/W412A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain P A This feature has been corrected as a result of our quality control procedures. The original label was 'P385A/E386A/W412A'. Wed May 02 19:42:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro385ala,glu386ala,trp412ala' Tue Jun 30 18:25:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro385Ala;Glu386Ala;Trp412Ala]' P35193:p.[Phe376Ala;Phe379Ala;Trp412Ala] F376A/F379A/W412A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F376A/F379A/W412A'. Wed May 02 19:02:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe376ala,phe379ala,trp412ala' Tue Jun 30 15:51:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe376Ala;Phe379Ala;Trp412Ala]' P35193:p.[Phe376Ala;Phe379Ala] F376A/F379A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F376A/F379A'. Wed May 02 15:20:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe376ala,phe379ala' Tue Jun 30 17:52:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe376Ala;Phe379Ala]' P35193:p.[Phe379Ala;Trp412Ala] F379A/W412A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F379A/W412A'. Wed May 02 14:55:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe379ala,trp412ala' Tue Jun 30 19:54:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe379Ala;Trp412Ala]' ecolx Escherichia coli 15 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction atg34-atg8-2 9 14 unspecified role unspecified role unspecified role unspecified role Q12292:p.Trp409Ala W409A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W409A'. Wed May 02 17:06:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp409ala' Tue Jun 30 17:56:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp409Ala' ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction ape1-atg19-1 5 13 unspecified role unspecified role bait bait Region 1-45 Region 1-45 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 16 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction atg11-atg19-2 3 16 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 12 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association atg8-atg19-2 1 16 unspecified role unspecified role prey prey P35193:p.Trp412Ala W412A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W412A'. Wed May 02 19:38:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp412ala' Tue Jun 30 20:05:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp412Ala' P35193:p.[Phe376Ala;Phe379Ala;Trp412Ala] F376A/F379A/W412A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F376A/F379A/W412A'. Wed May 02 14:50:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe376ala,phe379ala,trp412ala' Tue Jun 30 16:55:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe376Ala;Phe379Ala;Trp412Ala]' P35193:p.[Phe376Ala;Phe379Ala;Pro385Ala;Glu386Ala;Trp412Ala] F376A/F379A/P385A/E386A/W412A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F376A/F379A/P385A/E386A/W412A'. Wed May 02 17:00:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe376ala,phe379ala,pro385ala,glu386ala,trp412ala' Tue Jun 30 19:40:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe376Ala;Phe379Ala;Pro385Ala;Glu386Ala;Trp412Ala]' Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 15 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association