DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis of recognition of interferon-α receptor by tyrosine kinase 2. Structural basis of recognition of interferon-α receptor by tyrosine kinase 2. Nature structural & molecular biology 2014 imex curation Wallweber HJ., Tam C., Franke Y., Starovasnik MA., Lupardus PJ. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Wallweber HJ., Tam C., Franke Y., Starovasnik MA., Lupardus PJ. DIP original experiment: DIP-260875X Nature structural & molecular biology 2014 IMEx Structural basis of recognition of interferon-α receptor by tyrosine kinase 2. Structural basis of recognition of interferon-α receptor by tyrosine kinase 2. Structural basis of recognition of interferon-α receptor by tyrosine kinase 2. Nature structural & molecular biology 2014 imex curation Wallweber HJ., Tam C., Franke Y., Starovasnik MA., Lupardus PJ. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Wallweber HJ., Tam C., Franke Y., Starovasnik MA., Lupardus PJ. DIP original experiment: DIP-260876X Nature structural & molecular biology 2014 IMEx Structural basis of recognition of interferon-α receptor by tyrosine kinase 2. Structural basis of recognition of interferon-α receptor by tyrosine kinase 2. Structural basis of recognition of interferon-α receptor by tyrosine kinase 2. Nature structural & molecular biology 2014 imex curation Wallweber HJ., Tam C., Franke Y., Starovasnik MA., Lupardus PJ. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Wallweber HJ., Tam C., Franke Y., Starovasnik MA., Lupardus PJ. DIP original experiment: DIP-260877X Nature structural & molecular biology 2014 IMEx Structural basis of recognition of interferon-α receptor by tyrosine kinase 2. tyk2_human Non-receptor tyrosine-protein kinase TYK2 TYK2 protein protein human Homo sapiens Human MPLRHWGMARGSKPVGDGAQPMAAMGGLKVLLHWAGPGGGEPWVTFSESSLTAEEVCIHIAHKVGITPPCFNLFALFDAQAQVWLPPNHILEIPRDASLMLYFRIRFYFRNWHGMNPREPAVYRCGPPGTEASSDQTAQGMQLLDPASFEYLFEQGKHEFVNDVASLWELSTEEEIHHFKNESLGMAFLHLCHLALRHGIPLEEVAKKTSFKDCIPRSFRRHIRQHSALTRLRLRNVFRRFLRDFQPGRLSQQMVMVKYLATLERLAPRFGTERVPVCHLRLLAQAEGEPCYIRDSGVAPTDPGPESAAGPPTHEVLVTGTGGIQWWPVEEEVNKEEGSSGSSGRNPQASLFGKKAKAHKAVGQPADRPREPLWAYFCDFRDITHVVLKEHCVSIHRQDNKCLELSLPSRAAALSFVSLVDGYFRLTADSSHYLCHEVAPPRLVMSIRDGIHGPLLEPFVQAKLRPEDGLYLIHWSTSHPYRLILTVAQRSQAPDGMQSLRLRKFPIEQQDGAFVLEGWGRSFPSVRELGAALQGCLLRAGDDCFSLRRCCLPQPGETSNLIIMRGARASPRTLNLSQLSFHRVDQKEITQLSHLGQGTRTNVYEGRLRVEGSGDPEEGKMDDEDPLVPGRDRGQELRVVLKVLDPSHHDIALAFYETASLMSQVSHTHLAFVHGVCVRGPENIMVTEYVEHGPLDVWLRRERGHVPMAWKMVVAQQLASALSYLENKNLVHGNVCGRNILLARLGLAEGTSPFIKLSDPGVGLGALSREERVERIPWLAPECLPGGANSLSTAMDKWGFGATLLEICFDGEAPLQSRSPSEKEHFYQRQHRLPEPSCPQLATLTSQCLTYEPTQRPSFRTILRDLTRLQPHNLADVLTVNPDSPASDPTVFHKRYLKKIRDLGEGHFGKVSLYCYDPTNDGTGEMVAVKALKADCGPQHRSGWKQEIDILRTLYHEHIIKYKGCCEDQGEKSLQLVMEYVPLGSLRDYLPRHSIGLAQLLLFAQQICEGMAYLHAQHYIHRDLAARNVLLDNDRLVKIGDFGLAKAVPEGHEYYRVREDGDSPVFWYAPECLKEYKFYYASDVWSFGVTLYELLTHCDSSQSPPTKFLELIGIAQGQMTVLRLTELLERGERLPRPDKCPCEVYHLMKNCWETEASFRPTFENLIPILKTVHEKYQGQAPSVFSVC DIP protein P29597 original sequence version: 155 F76C25D6A919EDBC p17181-1 Interferon alpha/beta receptor 1 Cytokine receptor class-II member 1 Cytokine receptor family 2 member 1 Type I interferon receptor 1 IFNAR1 IFNAR protein protein human Homo sapiens Human MMVVLLGATTLVLVAVAPWVLSAAAGGKNLKSPQKVEVDIIDDNFILRWNRSDESVGNVTFSFDYQKTGMDNWIKLSGCQNITSTKCNFSSLKLNVYEEIKLRIRAEKENTSSWYEVDSFTPFRKAQIGPPEVHLEAEDKAIVIHISPGTKDSVMWALDGLSFTYSLVIWKNSSGVEERIENIYSRHKIYKLSPETTYCLKVKAALLTSWKIGVYSPVHCIKTTVENELPPPENIEVSVQNQNYVLKWDYTYANMTFQVQWLHAFLKRNPGNHLYKWKQIPDCENVKTTQCVFPQNVFQKGIYLLRVQASDGNNTSFWSEEIKFDTEIQAFLLPPVFNIRSLSDSFHIYIGAPKQSGNTPVIQDYPLIYEIIFWENTSNAERKIIEKKTDVTVPNLKPLTVYCVKARAHTMDEKLNKSSVFSDAVCEKTKPGNTSKIWLIVGICIALFALPFVIYAAKVFLRCINYVFFPSLKPSSSIDEYFSEQPLKNLLLSTSEEQIEKCFIIENISTIATVEETNQTDEDHKKYSSQTSQDSGNYSNEDESESKTSEELQQDFV DIP protein P17181-1 original sequence version: 137 tyk2-ifnar1-1 1 5 unspecified role unspecified role prey prey Region 465-512 Region 465-512 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P17181-1:p.Glu497Ala E497A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E497A'. Wed May 02 16:48:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu497ala' Tue Jun 30 17:58:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu497Ala' spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 4 unspecified role unspecified role bait bait Region 23-566 Region 23-566 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction ifnar1-tyk2-1 3 5 unspecified role unspecified role unspecified role unspecified role Region 465-512 Region 465-512 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 4 unspecified role unspecified role unspecified role unspecified role Region 23-566 Region 23-566 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction ifnar1-tyk2-2 2 5 unspecified role unspecified role bait bait Region 465-512 Region 465-512 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P17181-1:p.Glu497Ala E497A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E497A'. Wed May 02 15:18:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu497ala' Tue Jun 30 16:25:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu497Ala' spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 4 unspecified role unspecified role prey prey Region 23-566 Region 23-566 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction