DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of a type IV secretion system. Structure of a type IV secretion system. Nature 2014 imex curation Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. Only protein-protein interactions ecolx Escherichia coli pull down pull down anti tag western anti tag western blot anti tag western Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. DIP original experiment: DIP-260775X Nature 2014 IMEx Structure of a type IV secretion system. Structure of a type IV secretion system. Structure of a type IV secretion system. Nature 2014 imex curation Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. DIP original experiment: DIP-260770X Nature 2014 IMEx Structure of a type IV secretion system. Structure of a type IV secretion system. Structure of a type IV secretion system. Nature 2014 imex curation Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. Only protein-protein interactions ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. DIP original experiment: DIP-260766X Nature 2014 IMEx Structure of a type IV secretion system. Structure of a type IV secretion system. Structure of a type IV secretion system. Nature 2014 imex curation Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. Only protein-protein interactions ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. DIP original experiment: DIP-260774X Nature 2014 IMEx Structure of a type IV secretion system. Structure of a type IV secretion system. Structure of a type IV secretion system. Nature 2014 imex curation Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. Only protein-protein interactions ecolx Escherichia coli pull down pull down anti tag western anti tag western blot anti tag western Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. DIP original experiment: DIP-260777X Nature 2014 IMEx Structure of a type IV secretion system. Structure of a type IV secretion system. Structure of a type IV secretion system. Nature 2014 imex curation Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. DIP original experiment: DIP-260771X Nature 2014 IMEx Structure of a type IV secretion system. Structure of a type IV secretion system. Structure of a type IV secretion system. Nature 2014 imex curation Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. Only protein-protein interactions ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. DIP original experiment: DIP-260773X Nature 2014 IMEx Structure of a type IV secretion system. Structure of a type IV secretion system. Structure of a type IV secretion system. Nature 2014 imex curation Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. DIP original experiment: DIP-260765X Nature 2014 IMEx Structure of a type IV secretion system. Structure of a type IV secretion system. Structure of a type IV secretion system. Nature 2014 imex curation Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. Only protein-protein interactions ecolx Escherichia coli 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. DIP original experiment: DIP-260767X Nature 2014 IMEx Structure of a type IV secretion system. Structure of a type IV secretion system. Structure of a type IV secretion system. Nature 2014 imex curation Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. Only protein-protein interactions ecolx Escherichia coli pull down pull down anti tag western anti tag western blot anti tag western Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. DIP original experiment: DIP-260778X Nature 2014 IMEx Structure of a type IV secretion system. Structure of a type IV secretion system. Structure of a type IV secretion system. Nature 2014 imex curation Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. Only protein-protein interactions ecolx Escherichia coli ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. DIP original experiment: DIP-260769X Nature 2014 IMEx Structure of a type IV secretion system. Structure of a type IV secretion system. Structure of a type IV secretion system. Nature 2014 imex curation Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. Only protein-protein interactions ecolx Escherichia coli pull down pull down anti tag western anti tag western blot anti tag western Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. DIP original experiment: DIP-260776X Nature 2014 IMEx Structure of a type IV secretion system. Structure of a type IV secretion system. Structure of a type IV secretion system. Nature 2014 imex curation Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. DIP original experiment: DIP-260772X Nature 2014 IMEx Structure of a type IV secretion system. Structure of a type IV secretion system. Structure of a type IV secretion system. Nature 2014 imex curation Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Low HH., Gubellini F., Rivera-Calzada A., Braun N., Connery S., Dujeancourt A., Lu F., Redzej A., Fronzes R., Orlova EV., Waksman G. DIP original experiment: DIP-260768X Nature 2014 IMEx Structure of a type IV secretion system. o50335_ecolx trwG protein protein ecolx Escherichia coli MSKKQPKPVKAEQLKSYYEESRGLERDLIGEFVKSRKTAWRVATASGLFGLLGMVCGIVGFSQPAPAPLVLRVDNATGAVDVVTTLREHESSYGEVVDTYWLNQYVLNREAYDYNTIQMNYDTTALLSAPAVQQDYYKLFDGSNARDRVLGNKARITVRVRSIQPNGRGQATVRFTTQQHNSNGTVERPQHQIATIGYTYIGAPMRSSDRLLNPLGFQVTSYRADPEILNN DIP protein O50335 original sequence version: 45 o50329_ecolx trwM protein protein ecolx Escherichia coli MKPPQQQHEAFPLFKGATRLPTILGEPMIPLMAMVMGVAVIALTVSIWWWALVPPLWFIMAQITKNDDKAFRIWWLWIDTKFRNRNKGFWGASSYSPANYRKRR DIP protein O50329 original sequence version: 38 o50336_ecolx trwF protein protein ecolx Escherichia coli MKKLAIVALLASLHAVPALALDVPSSSRYDHRIRYVTYNPADVVQVDTVLGVATHIMLEEGEQYLTHAFGIPKPMPLPGRAGIFLSSRTAELANTNLIVVTDRRSYKFRLQMRNDRNGAMYELAFRYPDTQARQTREANARAAVEAAFEQRVGAYYNLKYMMSGDKDIAPVNAWDDGRFTYFKFSANADLPSIYFVDAEGNESLVPRTTVGSSNNIIAVHKVNPKWMIRLGNRALAIFNEAYDPNGVPNDTGTASPAVRRVNKGGN DIP protein O50336 original sequence version: 45 o50330_ecolx trwK protein protein ecolx Escherichia coli MGAIESRKLLASETPVGQFIPYSHHVTDTIISTKNAEYLSVWKIDGRSHQSASEADVFQWIRELNNTLRGISSANLSLWTHIVRRRVYEYPDAEFDNVFCRQLDEKYRESFTGYNLMVNDLYLTVVYRPVSDKVLSFFAKRERETPDQKKHRQESCIKALEDINRTLGQSFKRYGAELLSVYEKGGHAFSAPLEFLARLVNGEHIPMPICRDRFSDYMAVNRPMFSKWGEVGELRSLTGLRRFGMLEIREYDDATEPGQLNVLLESDYEFVLTHSFSVLSRPAAKEYLQRHQKNLIDARDVATDQIEEIDEALNQLISGHFVMGEHHCTLTVYGETVQQVRDNLAHASAAMLDVAVLPKPVDLALEAGYWAQLPANWQWRPRPAPITSLNFLSFSPFHNFMSGKPTGNPWGPAVTILKTVSGTPLYFNFHASKEEEDATDKRLLGNTMLIGQSSSGKTVLLGFLLAQAQKFKPTIVAFDKDRGMEISIRAMGGRYLPLKTGEPSGFNPFQLPPTHANLIFLKQFVKKLAAAGGEVTHRDEEEIDQAITAMMSDSIDKSLRRLSLLLQFLPNPRSDDMDARPTVHARLVKWCEGGDYGWLFDNPTDALDLSTHQIYGFDITEFLDNPEARTPVMMYLLYRTESMIDGRRFMYVFDEFWKPLQDEYFEDLAKNKQKTIRKQNGIFVFATQEPSDALESNIAKTLIQQCATYIFLANPKADYEDYTQGFKLTDSEFELVRGLGEFSRRFLIKQGDQSALAEMNLGKFRTIVDGETVERDFDDELLVLSGTPDNAEIAESIIAEVGDDPAVWLPIFLDRVKAERSDV DIP protein O50330 original sequence version: 43 q46702_ecolx traN protein protein ecolx Escherichia coli MRSLLLMGVLLISACSSGHKPPPEPDWSNTVPVNKTIPVDTQGGRNES 43ACC8EA9591FBAB 43ACC8EA9591FBAB o50334_ecolx trwH protein protein ecolx Escherichia coli MKTIIFAILMTGLLSACASAPKPKQPSDFNREPVNKTVPVEIQRGAL DIP protein O50334 original sequence version: 38 o50337_ecolx trwE protein protein ecolx Escherichia coli MFGRKKGDVIDAGAELERAEQERIEGEYGASELASERRPHTPGARTLLMVLLCVIAVVLVTLSYKAYKVRGVVEDDDAQPQQVVRQVIPGYTPRPIRPEPENVPEPPQPTTSVPAIQPAPVTQPVRPQPTGPREKTPYELARERMLRSGLTAGSGGGEDLPRPQGGDVPAGGLMGGGGGGGELAEKLQPMRLSGSSAGRLGNRDMLITQGTQLDCVLETRLVTTQPGMTTCHLTRDVYSTSGRVVLLDRGSKVVGFYQGGLRQGQARIFVQWSRIETPSGVVINLDSPGTGPLGEAGLGGWIDRHFWERFGGAIMISLIGDLGDWASRQGSRQGNNSIQFSNTANGVESAAAEALRNSINIPPTLYKNQGERVNILVARDLDFSDVYSLESIPTK DIP protein O50337 original sequence version: 45 15F74DE50FC3D521 q46705_ecolx traF protein protein ecolx Escherichia coli MARKSVDVDQELDENTGDGEFESERGGFNGSNRRSAPRMKAFVILMALLALVFIGITVMGKIRAPAKAEADKDGGKAQQANTLPNYSFNSDPDVNKPATAQNSPTDARAVQAAAQADADAGSSNTGARTSNKRKEPSPEELAMQRRLGGELAQTNQAATSNSPGAQPQDNETSEGSSALAKNLTPARLKASRAGVMANPSLTVPKGKMIPCGTGTELDTTVPGQVSCRVSQDVYSADGLVRLIDKGSWVDGQITGGIKDGQARVFVLWERIRNDQDGTIVNIDSAGTNSLGSAGIPGQVDAHMWERLAGAIMISLFSDTLTALVNQTQSNNIQYNSTENSGGQLASEALRSYMSIPPTLYDQQGDAVSIFVARDLDFSGVYTLADN 3F94CFD32ED8316B 3F94CFD32ED8316B q46704_ecolx traO protein protein ecolx Escherichia coli MKKLLLSAVVLSVLGGAATNVMALEVGRNSPYDYRIKSVVYNPVNVVKIDAVAGVATHIVVAPDETYITHAFGDSESRTFAHKMNHFFVKPKQAMSDTNLVIVTDKRTYNIVLHFIGEETKKNADGTVSKSFIETPWAVRHAVLQLTYEYPFEQQEKAKSAADKKRITQKLKQTAFAGAKNYQYVMSEQPEMRSIQPVHVWDNYRFTRFEFPANAELPQVYMISASGKETLPNSHVVGENRNIIEVETVAKEWRIRLGDKVVGVRNNNFAPGRGAVATGTASPDVRRVQIGEDN DCAE0E16EEB542FC DCAE0E16EEB542FC o50333_ecolx trwI protein protein ecolx Escherichia coli MAFELFTPLFNKIDQTTATYVTDISSRAIAAITPVVSVGLTLGFITYGWLIIRGAVEMPVAEFLNRCLRIGIIVSIALAGGLYQGEIANAITTVPDELASALLGNPTEGASAAALVDQSAQQGFDRASEAFEEAGFFSSDGLLYGLFGIIIPAGYGPAGAIGGAFLLLAKIALALLAGLGPLFILALIWQPTHRFFDQWAQQVLNYGLLIVLFAAVFGLLMQIFGSYMADLRFDGAQNVAYAIGGSVILSIVSIVLLMQLPSIASGLAGGIGLGYMWELRSMRSGAGAAMRGGRAMARGARAAPGAARGAAVGAANMAKTVATGGAGVARAAAGYFRGRKAG DIP protein O50333 original sequence version: 40 o50331_ecolx trwJ protein protein ecolx Escherichia coli MKKLVMTAAVAAILGAASPVMAQGIPVFDGTRALDFVQQFARMKEQLDTAKDQLAEAQRMYEAVTGGRGLGDLMRNAQLREYLPDDLRTVYDSANGGGYSGISGSINDILRDERLNGSVADMRRSIEERSRTARTTDKAVGCGPTKGHSNASRRLKDEISRTQDQKAIEELQARIAGEQAAIQNETTKLQMIAQLRQAEQALISEQRRERNMPILSSGNQGMPTIQ DIP protein O50331 original sequence version: 50 trwe-trwf-1 8 20 unspecified role unspecified role prey prey ecolx Escherichia coli 16 unspecified role unspecified role prey prey ecolx Escherichia coli 25 unspecified role unspecified role prey prey ecolx Escherichia coli 24 unspecified role unspecified role prey prey ecolx Escherichia coli 15 unspecified role unspecified role prey prey ecolx Escherichia coli 17 unspecified role unspecified role prey prey ecolx Escherichia coli 18 unspecified role unspecified role prey prey ecolx Escherichia coli 21 unspecified role unspecified role bait bait StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli association association trwk-trwm-1 2 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction traf-tran-5 4 19 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 23 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 22 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association trwe-trwf-2 9 20 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 25 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 24 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 21 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association trwe-trwm-1 1 16 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 21 unspecified role unspecified role bait bait StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli physical association physical association trwe-trwj-1 10 25 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 21 unspecified role unspecified role bait bait StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli physical association physical association trwe-trwf-3 6 20 unspecified role unspecified role prey prey ecolx Escherichia coli 17 unspecified role unspecified role prey prey ecolx Escherichia coli 21 unspecified role unspecified role bait bait StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli association association trwe-trwf-4 13 20 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 21 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association trwe-trwg-1 12 15 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 21 unspecified role unspecified role bait bait StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli physical association physical association trwe-trwf-5 3 20 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 25 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 24 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 21 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association trwe-trwf-6 7 20 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 21 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association trwk-trwm-2 14 16 unspecified role unspecified role prey prey ecolx Escherichia coli 18 unspecified role unspecified role bait bait StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli physical association physical association trwe-trwi-1 5 24 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 21 unspecified role unspecified role bait bait StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli physical association physical association trwk-trwm-3 11 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction