DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A promiscuous intermediate underlies the evolution of LEAFY DNA binding specificity. A promiscuous intermediate underlies the evolution of LEAFY DNA binding specificity. Science (New York, N.Y.) 2014 imex curation Sayou C., Monniaux M., Nanao MH., Moyroud E., Brockington SF., Thévenon E., Chahtane H., Warthmann N., Melkonian M., Zhang Y., Wong GK., Weigel D., Parcy F., Dumas R. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Sayou C., Monniaux M., Nanao MH., Moyroud E., Brockington SF., Thévenon E., Chahtane H., Warthmann N., Melkonian M., Zhang Y., Wong GK., Weigel D., Parcy F., Dumas R. DIP original experiment: DIP-264666X Science (New York, N.Y.) 2014 IMEx A promiscuous intermediate underlies the evolution of LEAFY DNA binding specificity. q94if5_phypa Floricaula/leafy-like transcription factor PPLFY1 PpLFY1 PHYPADRAFT_163123 protein protein phypa Physcomitrella patens Moss MVDDCPVHNMAQFDIVQAEPTALLYKRPDGVALVGRENRRLKELFKDFGVQPSTVAVMGQMGFTLSTLINMKDEEVDFVIKSMIEEYHLDLLMGEQFGIKAAVRAKRRLVEEEMEQQRMDLAARSNEKKRRLEESSDGLLLKDGNGSLEALGLLGLTDPDSDSEGKKVAKRRQRKRRSKEVGEDGEERPREHPFIVTEPGEPAKGKKNGLDYLFDLYEQCGKFLEEVQHIAKEKGEKCPSKVTNEVFRHAKLTGAGYINKPKMRDYVHCYALHCLDVETSNNLRKEYKERGENVGAWCQACYFPLVKLARQNEWDIDDLFNRNDKLRIWYVPTKLRQLCHIERMKHGE DIP protein Q94IF5 original sequence version: 59 pplfy1-1 1 2 unspecified role unspecified role unspecified role unspecified role Region 180-347 Region 180-347 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction