DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation. Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation. Structure (London, England : 1993) 2014 imex curation Mikami S., Kanaba T., Takizawa N., Kobayashi A., Maesaki R., Fujiwara T., Ito Y., Mishima M. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Mikami S., Kanaba T., Takizawa N., Kobayashi A., Maesaki R., Fujiwara T., Ito Y., Mishima M. DIP original experiment: DIP-259601X Structure (London, England : 1993) 2014 IMEx Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation. Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation. Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation. Structure (London, England : 1993) 2014 imex curation Mikami S., Kanaba T., Takizawa N., Kobayashi A., Maesaki R., Fujiwara T., Ito Y., Mishima M. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Mikami S., Kanaba T., Takizawa N., Kobayashi A., Maesaki R., Fujiwara T., Ito Y., Mishima M. DIP original experiment: DIP-259600X Structure (London, England : 1993) 2014 IMEx Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation. Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation. Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation. Structure (London, England : 1993) 2014 imex curation Mikami S., Kanaba T., Takizawa N., Kobayashi A., Maesaki R., Fujiwara T., Ito Y., Mishima M. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Mikami S., Kanaba T., Takizawa N., Kobayashi A., Maesaki R., Fujiwara T., Ito Y., Mishima M. DIP original experiment: DIP-259602X Structure (London, England : 1993) 2014 IMEx Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation. Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation. Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation. Structure (London, England : 1993) 2014 imex curation Mikami S., Kanaba T., Takizawa N., Kobayashi A., Maesaki R., Fujiwara T., Ito Y., Mishima M. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Mikami S., Kanaba T., Takizawa N., Kobayashi A., Maesaki R., Fujiwara T., Ito Y., Mishima M. DIP original experiment: DIP-259603X Structure (London, England : 1993) 2014 IMEx Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation. Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation. Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation. Structure (London, England : 1993) 2014 imex curation Mikami S., Kanaba T., Takizawa N., Kobayashi A., Maesaki R., Fujiwara T., Ito Y., Mishima M. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Mikami S., Kanaba T., Takizawa N., Kobayashi A., Maesaki R., Fujiwara T., Ito Y., Mishima M. DIP original experiment: DIP-259604X Structure (London, England : 1993) 2014 IMEx Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation. ncor2_human Nuclear receptor corepressor 2 NCOR2 CTG26 Silencing mediator of retinoic acid and thyroid hormone receptor Thyroid-, retinoic-acid-receptor-associated corepressor T3 receptor-associating factor CTG repeat protein 26 SMAP270 protein protein human Homo sapiens Human MSGSTQPVAQTWRATEPRYPPHSLSYPVQIARTHTDVGLLEYQHHSRDYASHLSPGSIIQPQRRRPSLLSEFQPGNERSQELHLRPESHSYLPELGKSEMEFIESKRPRLELLPDPLLRPSPLLATGQPAGSEDLTKDRSLTGKLEPVSPPSPPHTDPELELVPPRLSKEELIQNMDRVDREITMVEQQISKLKKKQQQLEEEAAKPPEPEKPVSPPPIESKHRSLVQIIYDENRKKAEAAHRILEGLGPQVELPLYNQPSDTRQYHENIKINQAMRKKLILYFKRRNHARKQWEQKFCQRYDQLMEAWEKKVERIENNPRRRAKESKVREYYEKQFPEIRKQRELQERMQSRVGQRGSGLSMSAARSEHEVSEIIDGLSEQENLEKQMRQLAVIPPMLYDADQQRIKFINMNGLMADPMKVYKDRQVMNMWSEQEKETFREKFMQHPKNFGLIASFLERKTVAECVLYYYLTKKNENYKSLVRRSYRRRGKSQQQQQQQQQQQQQQQQQPMPRSSQEEKDEKEKEKEAEKEEEKPEVENDKEDLLKEKTDDTSGEDNDEKEAVASKGRKTANSQGRRKGRITRSMANEANSEEAITPQQSAELASMELNESSRWTEEEMETAKKGLLEHGRNWSAIARMVGSKTVSQCKNFYFNYKKRQNLDEILQQHKLKMEKERNARRKKKKAPAAASEEAAFPPVVEDEEMEASGVSGNEEEMVEEAEALHASGNEVPRGECSGPATVNNSSDTESIPSPHTEAAKDTGQNGPKPPATLGADGPPPGPPTPPPEDIPAPTEPTPASEATGAPTPPPAPPSPSAPPPVVPKEEKEEETAAAPPVEEGEEQKPPAAEELAVDTGKAEEPVKSECTEEAEEGPAKGKDAEAAEATAEGALKAEKKEGGSGRATTAKSSGAPQDSDSSATCSADEVDEAEGGDKNRLLSPRPSLLTPTGDPRANASPQKPLDLKQLKQRAAAIPPIQVTKVHEPPREDAAPTKPAPPAPPPPQNLQPESDAPQQPGSSPRGKSRSPAPPADKEAFAAEAQKLPGDPPCWTSGLPFPVPPREVIKASPHAPDPSAFSYAPPGHPLPLGLHDTARPVLPRPPTISNPPPLISSAKHPSVLERQIGAISQGMSVQLHVPYSEHAKAPVGPVTMGLPLPMDPKKLAPFSGVKQEQLSPRGQAGPPESLGVPTAQEASVLRGTALGSVPGGSITKGIPSTRVPSDSAITYRGSITHGTPADVLYKGTITRIIGEDSPSRLDRGREDSLPKGHVIYEGKKGHVLSYEGGMSVTQCSKEDGRSSSGPPHETAAPKRTYDMMEGRVGRAISSASIEGLMGRAIPPERHSPHHLKEQHHIRGSITQGIPRSYVEAQEDYLRREAKLLKREGTPPPPPPSRDLTEAYKTQALGPLKLKPAHEGLVATVKEAGRSIHEIPREELRHTPELPLAPRPLKEGSITQGTPLKYDTGASTTGSKKHDVRSLIGSPGRTFPPVHPLDVMADARALERACYEESLKSRPGTASSSGGSIARGAPVIVPELGKPRQSPLTYEDHGAPFAGHLPRGSPVTTREPTPRLQEGSLSSSKASQDRKLTSTPREIAKSPHSTVPEHHPHPISPYEHLLRGVSGVDLYRSHIPLAFDPTSIPRGIPLDAAAAYYLPRHLAPNPTYPHLYPPYLIRGYPDTAALENRQTIINDYITSQQMHHNAATAMAQRADMLRGLSPRESSLALNYAAGPRGIIDLSQVPHLPVLVPPTPGTPATAMDRLAYLPTAPQPFSSRHSSSPLSPGGPTHLTKPTTTSSSERERDRDRERDRDREREKSILTSTTTVEHAPIWRPGTEQSSGSSGGGGGSSSRPASHSHAHQHSPISPRTQDALQQRPSVLHNTGMKGIITAVEPSTPTVLRSTSTSSPVRPAATFPPATHCPLGGTLDGVYPTLMEPVLLPKEAPRVARPERPRADTGHAFLAKPPARSGLEPASSPSKGSEPRPLVPPVSGHATIARTPAKNLAPHHASPDPPAPPASASDPHREKTQSKPFSIQELELRSLGYHGSSYSPEGVEPVSPVSSPSLTHDKGLPKHLEELDKSHLEGELRPKQPGPVKLGGEAAHLPHLRPLPESQPSSSPLLQTAPGVKGHQRVVTLAQHISEVITQDYTRHHPQQLSAPLPAPLYSFPGASCPVLDLRRPPSDLYLPPPDHGAPARGSPHSEGGKRSPEPNKTSVLGGGEDGIEPVSPPEGMTEPGHSRSAVYPLLYRDGEQTEPSRMGSKSPGNTSQPPAFFSKLTESNSAMVKSKKQEINKKLNTHNRNEPEYNISQPGTEIFNMPAITGTGLMTYRSQAVQEHASTNMGLEAIIRKALMGKYDQWEESPPLSANAFNPLNASASLPAAMPITAADGRSDHTLTSPGGGGKAKVSGRPSSRKAKSPAPGLASGDRPPSVSSVHSEGDCNRRTPLTNRVWEDRPSSAGSTPFPYNPLIMRLQAGVMASPPPPGLPAGSGPLAGPHHAWDEEPKPLLCSQYETLSDSE DIP protein Q9Y618 original sequence version: 166 DIP protein Q9Y618-1 original sequence version: 119 DIP protein Q9Y618-1 original sequence version: 155 DIP protein Q9Y618 original sequence version: 134 DIP protein Q9Y618 original sequence version: 138 70A89C9A19612AAD mint_human Msx2-interacting protein SPEN homolog SMART/HDAC1-associated repressor protein KIAA0929 MINT SHARP SPEN protein protein human Homo sapiens Human MVRETRHLWVGNLPENVREEKIIEHFKRYGRVESVKILPKRGSEGGVAAFVDFVDIKSAQKAHNSVNKMGDRDLRTDYNEPGTIPSAARGLDDTVSIASRSREVSGFRGGGGGPAYGPPPSLHAREGRYERRLDGASDNRERAYEHSAYGHHERGTGGFDRTRHYDQDYYRDPRERTLQHGLYYASRSRSPNRFDAHDPRYEPRAREQFTLPSVVHRDIYRDDITREVRGRRPERNYQHSRSRSPHSSQSRNQSPQRLASQASRPTRSPSGSGSRSRSSSSDSISSSSSTSSDSSDSSSSSSDDSPARSVQSAAVPAPTSQLLSSLEKDEPRKSFGIKVQNLPVRSTDTSLKDGLFHEFKKFGKVTSVQIHGTSEERYGLVFFRQQEDQEKALTASKGKLFFGMQIEVTAWIGPETESENEFRPLDERIDEFHPKATRTLFIGNLEKTTTYHDLRNIFQRFGEIVDIDIKKVNGVPQYAFLQYCDIASVCKAIKKMDGEYLGNNRLKLGFGKSMPTNCVWLDGLSSNVSDQYLTRHFCRYGPVVKVVFDRLKGMALVLYNEIEYAQAAVKETKGRKIGGNKIKVDFANRESQLAFYHCMEKSGQDIRDFYEMLAERREERRASYDYNQDRTYYESVRTPGTYPEDSRRDYPARGREFYSEWETYQGDYYESRYYDDPREYRDYRNDPYEQDIREYSYRQRERERERERFESDRDRDHERRPIERSQSPVHLRRPQSPGASPSQAERLPSDSERRLYSRSSDRSGSCSSLSPPRYEKLDKSRLERYTKNEKTDKERTFDPERVERERRLIRKEKVEKDKTDKQKRKGKVHSPSSQSSETDQENEREQSPEKPRSCNKLSREKADKEGIAKNRLELMPCVVLTRVKEKEGKVIDHTPVEKLKAKLDNDTVKSSALDQKLQVSQTEPAKSDLSKLESVRMKVPKEKGLSSHVEVVEKEGRLKARKHLKPEQPADGVSAVDLEKLEARKRRFADSNLKAEKQKPEVKKSSPEMEDARVLSKKQPDVSSREVILLREGEAERKPVRKEILKRESKKIKLDRLNTVASPKDCQELASISVGSGSRPSSDLQARLGELAGESVENQEVQSKKPIPSKPQLKQLQVLDDQGPEREDVRKNYCSLRDETPERKSGQEKSHSVNTEEKIGIDIDHTQSYRKQMEQSRRKQQMEMEIAKSEKFGSPKKDVDEYERRSLVHEVGKPPQDVTDDSPPSKKKRMDHVDFDICTKRERNYRSSRQISEDSERTGGSPSVRHGSFHEDEDPIGSPRLLSVKGSPKVDEKVLPYSNITVREESLKFNPYDSSRREQMADMAKIKLSVLNSEDELNRWDSQMKQDAGRFDVSFPNSIIKRDSLRKRSVRDLEPGEVPSDSDEDGEHKSHSPRASALYESSRLSFLLRDREDKLRERDERLSSSLERNKFYSFALDKTITPDTKALLERAKSLSSSREENWSFLDWDSRFANFRNNKDKEKVDSAPRPIPSWYMKKKKIRTDSEGKMDDKKEDHKEEEQERQELFASRFLHSSIFEQDSKRLQHLERKEEDSDFISGRIYGKQTSEGANSTTDSIQEPVVLFHSRFMELTRMQQKEKEKDQKPKEVEKQEDTENHPKTPESAPENKDSELKTPPSVGPPSVTVVTLESAPSALEKTTGDKTVEAPLVTEEKTVEPATVSEEAKPASEPAPAPVEQLEQVDLPPGADPDKEAAMMPAGVEEGSSGDQPPYLDAKPPTPGASFSQAESNVDPEPDSTQPLSKPAQKSEEANEPKAEKPDATADAEPDANQKAEAAPESQPPASEDLEVDPPVAAKDKKPNKSKRSKTPVQAAAVSIVEKPVTRKSERIDREKLKRSNSPRGEAQKLLELKMEAEKITRTASKNSAADLEHPEPSLPLSRTRRRNVRSVYATMGDHENRSPVKEPVEQPRVTRKRLERELQEAAAVPTTPRRGRPPKTRRRADEEEENEAKEPAETLKPPEGWRSPRSQKTAAGGGPQGKKGKNEPKVDATRPEATTEVGPQIGVKESSMEPKAAEEEAGSEQKRDRKDAGTDKNPPETAPVEVVEKKPAPEKNSKSKRGRSRNSRLAVDKSASLKNVDAAVSPRGAAAQAGERESGVVAVSPEKSESPQKEDGLSSQLKSDPVDPDKEPEKEDVSASGPSPEATQLAKQMELEQAVEHIAKLAEASASAAYKADAPEGLAPEDRDKPAHQASETELAAAIGSIINDISGEPENFPAPPPYPGESQTDLQPPAGAQALQPSEEGMETDEAVSGILETEAATESSRPPVNAPDPSAGPTDTKEARGNSSETSHSVPEAKGSKEVEVTLVRKDKGRQKTTRSRRKRNTNKKVVAPVESHVPESNQAQGESPAANEGTTVQHPEAPQEEKQSEKPHSTPPQSCTSDLSKIPSTENSSQEISVEERTPTKASVPPDLPPPPQPAPVDEEPQARFRVHSIIESDPVTPPSDPSIPIPTLPSVTAAKLSPPVASGGIPHQSPPTKVTEWITRQEEPRAQSTPSPALPPDTKASDVDTSSSTLRKILMDPKYVSATSVTSTSVTTAIAEPVSAAPCLHEAPPPPVDSKKPLEEKTAPPVTNNSEIQASEVLVAADKEKVAPVIAPKITSVISRMPVSIDLENSQKITLAKPAPQTLTGLVSALTGLVNVSLVPVNALKGPVKGSVTTLKSLVSTPAGPVNVLKGPVNVLTGPVNVLTTPVNATVGTVNAAPGTVNAAASAVNATASAVTVTAGAVTAASGGVTATTGTVTMAGAVIAPSTKCKQRASANENSRFHPGSMPVIDDRPADAGSGAGLRVNTSEGVVLLSYSGQKTEGPQRISAKISQIPPASAMDIEFQQSVSKSQVKPDSVTASQPPSKGPQAPAGYANVATHSTLVLTAQTYNASPVISSVKADRPSLEKPEPIHLSVSTPVTQGGTVKVLTQGINTPPVLVHNQLVLTPSIVTTNKKLADPVTLKIETKVLQPANLGSTLTPHHPPALPSKLPTEVNHVPSGPSIPADRTVSHLAAAKLDAHSPRPSGPGPSSFPRASHPSSTASTALSTNATVMLAAGIPVPQFISSIHPEQSVIMPPHSITQTVSLSHLSQGEVRMNTPTLPSITYSIRPEALHSPRAPLQPQQIEVRAPQRASTPQPAPAGVPALASQHPPEEEVHYHLPVARATAPVQSEVLVMQSEYRLHPYTVPRDVRIMVHPHVTAVSEQPRAADGVVKVPPASKAPQQPGKEAAKTPDAKAAPTPTPAPVPVPVPLPAPAPAPHGEARILTVTPSNQLQGLPLTPPVVVTHGVQIVHSSGELFQEYRYGDIRTYHPPAQLTHTQFPAASSVGLPSRTKTAAQGPPPEGEPLQPPQPVQSTQPAQPAPPCPPSQLGQPGQPPSSKMPQVSQEAKGTQTGVEQPRLPAGPANRPPEPHTQVQRAQAETGPTSFPSPVSVSMKPDLPVSLPTQTAPKQPLFVPTTSGPSTPPGLVLPHTEFQPAPKQDSSPHLTSQRPVDMVQLLKKYPIVWQGLLALKNDTAAVQLHFVSGNNVLAHRSLPLSEGGPPLRIAQRMRLEATQLEGVARRMTVETDYCLLLALPCGRDQEDVVSQTESLKAAFITYLQAKQAAGIINVPNPGSNQPAYVLQIFPPCEFSESHLSRLAPDLLASISNISPHLMIVIASV DIP protein Q96T58 original sequence version: 132 5228C58533E5B27B ncor2-spen-1 2 6 unspecified role unspecified role unspecified role unspecified role Region 2502-2525 Region 2502-2525 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pS2522/2524 pS2522/2524 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain in vitro In vitro 7 unspecified role unspecified role unspecified role unspecified role Region 3496-3664 Region 3496-3664 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ncor2-spen-2 3 6 unspecified role unspecified role bait bait Region 2502-2525 Region 2502-2525 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pS2522/2524 pS2522/2524 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain increasing-ptm ptm increasing an interaction increasing-ptm pS2522 pS2522 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain increasing-ptm ptm increasing an interaction increasing-ptm pS2524 pS2524 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain increasing-ptm ptm increasing an interaction increasing-ptm in vitro In vitro 7 unspecified role unspecified role prey prey Region 3496-3664 Region 3496-3664 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q96T58:p.Lys3516Ala K3516A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K3516A'. Wed May 02 16:13:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys3516ala' Tue Jun 30 15:51:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys3516Ala' Q96T58:p.Arg3552Ala R3552A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R3552A'. Wed May 02 16:23:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg3552ala' Tue Jun 30 16:45:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg3552Ala' Q96T58:p.Lys3606Ala K3606A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K3606A'. Wed May 02 19:13:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys3606ala' Tue Jun 30 17:05:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys3606Ala' Q96T58:p.Leu3515Ala L3515A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L3515A'. Wed May 02 19:47:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu3515ala' Tue Jun 30 19:01:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu3515Ala' Q96T58:p.Arg3548Ala R3548A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R3548A'. Wed May 02 16:40:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg3548ala' Tue Jun 30 17:41:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg3548Ala' Q96T58:p.Gln3551Ala Q3551A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Q A This feature has been corrected as a result of our quality control procedures. The original label was 'Q3551A'. Wed May 02 17:25:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln3551ala' Tue Jun 30 19:45:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln3551Ala' Q96T58:p.Arg3552Lys R3552K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R K This feature has been corrected as a result of our quality control procedures. The original label was 'R3552K'. Wed May 02 14:52:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg3552lys' Tue Jun 30 20:25:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg3552Lys' Q96T58:p.Met3553Ala M3553A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain M A This feature has been corrected as a result of our quality control procedures. The original label was 'M3553A'. Wed May 02 19:46:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met3553ala' Tue Jun 30 18:26:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met3553Ala' Q96T58:p.Arg3554Ala R3554A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R3554A'. Wed May 02 15:40:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg3554ala' Tue Jun 30 18:50:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg3554Ala' Q96T58:p.Tyr3602Ala Y3602A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y3602A'. Wed May 02 16:52:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr3602ala' Tue Jun 30 19:52:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr3602Ala' ecolx Escherichia coli direct interaction direct interaction spen-ncor2-1 5 6 unspecified role unspecified role prey prey Region 2518-2525 Region 2518-2525 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pS2522/2524 pS2522/2524 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain Q9Y618:p.Tyr2507Ala Y2518A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y2518A'. Wed May 02 19:42:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr2518ala' Wed Sep 26 11:44:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr2518Ala' Tue Jun 30 16:41:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr2507Ala' Q9Y618:p.[Tyr2507Ala;Glu2508Ala] Y2518A/E2519A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y2518A/E2519A'. Wed May 02 15:25:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr2518ala,glu2519ala' Wed Sep 26 13:30:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr2518Ala;Glu2519Ala]' Tue Jun 30 19:01:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr2507Ala;Glu2508Ala]' in vitro In vitro 7 unspecified role unspecified role bait bait Region 3496-3664 Region 3496-3664 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ncor2-spen-3 1 6 unspecified role unspecified role unspecified role unspecified role Region 2518-2525 Region 2518-2525 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pS2522/2524 pS2522/2524 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain in vitro In vitro 7 unspecified role unspecified role unspecified role unspecified role Region 3496-3664 Region 3496-3664 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ncor2-spen-4 4 6 unspecified role unspecified role unspecified role unspecified role Region 2518-2525 Region 2518-2525 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pS2522/2524 pS2522/2524 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain in vitro In vitro 7 unspecified role unspecified role unspecified role unspecified role Region 3496-3664 Region 3496-3664 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction