DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface. Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface. Structure (London, England : 1993) 2013 imex curation Lukhele S., Bah A., Lin H., Sonenberg N., Forman-Kay JD. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Lukhele S., Bah A., Lin H., Sonenberg N., Forman-Kay JD. DIP original experiment: DIP-255881X Structure (London, England : 1993) 2013 IMEx Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface. Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface. Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface. Structure (London, England : 1993) 2013 imex curation Lukhele S., Bah A., Lin H., Sonenberg N., Forman-Kay JD. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lukhele S., Bah A., Lin H., Sonenberg N., Forman-Kay JD. DIP original experiment: DIP-255882X Structure (London, England : 1993) 2013 IMEx Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface. if4e_human Eukaryotic translation initiation factor 4E mRNA cap-binding protein eIF-4F 25 kDa subunit EIF4E EIF4EL1 EIF4F protein protein human Homo sapiens Human MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV DIP protein P06730 original sequence version: 164 DIP protein P06730 original sequence version: 127 DIP protein P06730 original sequence version: 126 DIP protein P06730 original sequence version: 135 DIP protein P06730 original sequence version: 151 DIP protein P06730 original sequence version: 155 DIP protein P06730 original sequence version: 160 DIP protein P06730-1 original sequence version: 187 DIP protein P06730 original sequence version: 146 B869B8DE615E699D 4ebp2_human Eukaryotic translation initiation factor 4E-binding protein 2 EIF4EBP2 protein protein human Homo sapiens Human MSSSAGSGHQPSQSRAIPTRTVAISDAAQLPHDYCTTPGGTLFSTTPGGTRIIYDRKFLLDRRNSPMAQTPPCHLPNIPGVTSPGTLIEDSKVEVNNLNNLNNHDRKHAVGDDAQFEMDI DIP protein Q13542 original sequence version: 106 B8F109261A504193 eif4e-eif4ebp2-7 1 3 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role Q13542:p.[Tyr54Ala;Leu59Ala;Ile78Ala;Pro79Ala;Gly80Ala;Val81Ala;Thr82Ala] Y54A/L59A/I78A/P79A/G80A/V81A/T82A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain V A certain certain sequence position certain certain certain sequence position certain G A certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y54A/L59A/I78A/P79A/G80A/V81A/T82A'. Wed May 02 19:07:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr54ala,leu59ala,ile78ala,pro79ala,gly80ala,val81ala,thr82ala' Tue Jun 30 17:26:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr54Ala;Leu59Ala;Ile78Ala;Pro79Ala;Gly80Ala;Val81Ala;Thr82Ala]' ecolx Escherichia coli direct interaction direct interaction eif4e-eif4ebp2-8 2 3 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role Q13542:p.[Ile78Ala;Pro79Ala;Gly80Ala;Val81Ala;Thr82Ala] I78A/P79A/G80A/V81A/T82A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain V A certain certain sequence position certain certain certain sequence position certain G A certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I78A/P79A/G80A/V81A/T82A'. Wed May 02 19:09:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile78ala,pro79ala,gly80ala,val81ala,thr82ala' Tue Jun 30 18:05:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile78Ala;Pro79Ala;Gly80Ala;Val81Ala;Thr82Ala]' Q13542:p.[Tyr54Ala;Leu59Ala] Y54A/L59A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y54A/L59A'. Wed May 02 20:26:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr54ala,leu59ala' Tue Jun 30 20:12:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr54Ala;Leu59Ala]' ecolx Escherichia coli direct interaction direct interaction Deletion of region 78-82 decreased the interaction rate/strength.