DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. PLoS biology 2013 imex curation Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. DIP original experiment: DIP-260024X PLoS biology 2013 IMEx A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. PLoS biology 2013 imex curation Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. DIP original experiment: DIP-260023X PLoS biology 2013 IMEx A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. PLoS biology 2013 imex curation Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. DIP original experiment: DIP-260022X PLoS biology 2013 IMEx A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. PLoS biology 2013 imex curation Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. DIP original experiment: DIP-260025X PLoS biology 2013 IMEx A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. PLoS biology 2013 imex curation Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. DIP original experiment: DIP-260021X PLoS biology 2013 IMEx A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. PLoS biology 2013 imex curation Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. DIP original experiment: DIP-260020X PLoS biology 2013 IMEx A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. PLoS biology 2013 imex curation Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. DIP original experiment: DIP-260019X PLoS biology 2013 IMEx A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. cul1_drome Cullin homolog 1 Cul1 Lin-19 homolog protein cul-1 lin19 CG1877 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNRSGNSQTTQKLVNLDDIWSELVEGIMQVFEHEKSLTRSQYMRFYTHVYDYCTSVSAAPSGRSSGKTGGAQLVGKKLYDRLEQFLKSYLSELLTKFKAISGEEVLLSRYTKQWKSYQFSSTVLDGICNYLNRNWVKRECEEGQKGIYKIYRLALVAWKGHLFQVLNEPVTKAVLKSIEEERQGKLINRSLVRDVIECYVELSFNEEDTDAEQQKLSVYKQNFENKFIADTSAFYEKESDAFLSTNTVTEYLKHVENRLEEETQRVRGFNSKNGLSYLHETTADVLKSTCEEVLIEKHLKIFHTEFQNLLNADRNDDLKRMYSLVALSSKNLTDLKSILENHILHQGTEAIAKCCTTDAANDPKTYVQTILDVHKKYNALVLTAFNNDNGFVAALDKACGKFINSNVVTIANSASKSPELLAKYCDLLLKKSSKNPEDKELEDNLNQVMVVFKYIEDKDVFQKYYSKMLAKRLVNHTSASDDAEAMMISKLKQTCGYEYTVKLQRMFQDIGVSKDLNSYFKQYLAEKNLTMEIDFGIEVLSSGSWPFQLSNNFLLPSELERSVRQFNEFYAARHSGRKLNWLYQMCKGELIMNVNRNNSSTYTLQASTFQMSVLLQFNDQLSFTVQQLQDNTQTQQENLIQVLQILLKAKVLTSSDNENSLTPESTVELFLDYKNKKRRININQPLKTELKVEQETVHKHIEEDRKLLIQAAIVRIMKMRKRLNHTNLISEVLNQLSTRFKPKVPVIKKCIDILIEKEYLERMEGHKDTYSYLA DIP protein Q24311 original sequence version: 94 1AF0D035DE85363C o77430_drome SkpA Dredd EG:115C2.4 skpA skpA-RC skpA-RH Dmel\CG16983 SKP1 SKPA Skp Skp1 anon-WO03040301.234 anon-WO03040301.236 anon-WO03040301.238 dSKP1 dSkp-1 dSkpA dskpA l(1)G0037 l(1)G0058 l(1)G0109 l(1)G0389 spkA Dmel_CG16983 CG16983 dSkip-1 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPSIKLQSSDEEIFDTDIQIAKCSGTIKTMLEDCGMEDDENAIVPLPNVNSTILRKVLTWAHYHKDDPQPTEDDESKEKRTDDIISWDADFLKVDQGTLFELILAANYLDIKGLLELTCKTVANMIKGKTPEEIRKTFNIKKDFSPAEEEQVRKENEWCEEK DIP protein O77430 original sequence version: 87 DIP protein O77430 original sequence version: 114 FD218D30AC98CA0A akt1_drome RAC serine/threonine-protein kinase Akt1 CG4006 Akt Protein kinase B protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNYLPFVLQRRSTVVASAPAPGSASRIPESPTTTGSNIINIIYSQSTHPNSSPTSGSAEKFSWQQSWPSRTSAAPTHDSGTMSINTTFDLSSPSVTSGHALTEQTQVVKEGWLMKRGEHIKNWRQRYFVLHSDGRLMGYRSKPADSASTPSDFLLNNFTVRGCQIMTVDRPKPFTFIIRGLQWTTVIERTFAVESELERQQWTEAIRNVSSRLIDVGEVAMTPSEQTDMTDVDMATIAEDELSEQFSVQGTTCNSSGVKKVTLENFEFLKVLGKGTFGKVILCREKATAKLYAIKILKKEVIIQKDEVAHTLTESRVLKSTNHPFLISLKYSFQTNDRLCFVMQYVNGGELFWHLSHERIFTEDRTRFYGAEIISALGYLHSQGIIYRDLKLENLLLDKDGHIKVADFGLCKEDITYGRTTKTFCGTPEYLAPEVLDDNDYGQAVDWWGTGVVMYEMICGRLPFYNRDHDVLFTLILVEEVKFPRNITDEAKNLLAGLLAKDPKKRLGGGKDDVKEIQAHPFFASINWTDLVLKKIPPPFKPQVTSDTDTRYFDKEFTGESVELTPPDPTGPLGSIAEEPLFPQFSYQGDMASTLGTSSHISTSTSLASMQ DIP protein Q8INB9 original sequence version: 105 C139380152580934 rbx1a_drome RING-box protein 1A Roc1a CG16982 Regulator of cullins 1a dRbx1 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MEVDEDGYEVPSSSSKGDKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWDFQKYGH DIP protein Q9W5E1 original sequence version: 102 15784198281BCD13 q9vde3_drome slmb beta-TrCP BcDNA:GM02031 Beta-TRCP1 Dmel\CG3412 FBXW1 MENE (3R)-B MENE(3R)-B SLIMB SLMB Slimb Slmb beta-TRCP betaTrCP crd ica l(3)00295 shv slimb wel Dmel_CG3412 CG3412 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MMKMETDKIMDETNSNAQAFTTTMLYDPVRKKDSSPTYQTERELCFQYFTQWSESGQVDFVEHLLSRMCHYQHGQINAYLKPMLQRDFITLLPIKGLDHIAENILSYLDAESLKSSELVCKEWLRVISEGMLWKKLIERKVRTDSLWRGLAERRNWMQYLFKPRPGQTQRPHSFHRELFPKIMNDIDSIENNWRTGRHMLRRINCRSENSKGVYCLQYDDGKIVSGLRDNTIKIWDRTDLQCVKTLMGHTGSVLCLQYDDKVIISGSSDSTVRVWDVNTGEMVNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMTSPSEITLRRVLVGHRAAVNVVDFDEKYIVSASGDRTIKVWSTSSCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNSIRLWDIECGACLRVLEGHEELVRCIRFDTKRIVSGAYDGKIKVWDLVAALDPRAASNTLCLNTLVEHTGRVFRLQFDEFQIVSSSHDDTILIWDFLNFTPNENKTGRTPSPALMEH DIP protein Q9VDE3 original sequence version: 100 DIP protein Q9VDE3 original sequence version: 116 DIP protein Q9VDE3 original sequence version: 118 DIP protein Q9VDE3 original sequence version: 135 F4D5DF126F58A012 ubiq_drome Ubiquitin RpL40 CG2960 RpS27A UBI-F80 Ubi-f CG5271 Ubi-p63E Ubi-m CG11624 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG C42A35397FFD9B52 slmb-cul1-1 7 9 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 12 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly association association slmb-akt1-1 2 10 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 12 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 196-510 Region 196-510 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association akt1-slmb-1 5 10 unspecified role unspecified role bait bait drome Drosophila melanogaster (Fruit fly) Fruit fly 12 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association akt1-ub-2 4 13 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal drome Drosophila melanogaster (Fruit fly) Fruit fly 10 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association slmb-cul1-2 6 11 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 9 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 12 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly association association akt1-slmb-2 1 13 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal drome Drosophila melanogaster (Fruit fly) Fruit fly 10 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 12 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly association association slmb-akt1-2 3 10 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 12 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association