IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Human LilrB2 is a β-amyloid receptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's model. Human LilrB2 is a β-amyloid receptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's model. Science (New York, N.Y.) 2013 imex curation Kim T., Vidal GS., Djurisic M., William CM., Birnbaum ME., Garcia KC., Hyman BT., Shatz CJ. For several figs - IK, 2B + more; there is no detection of one component - so interactions not entered. From the "DNA Constructs" section in Supplement it is not clear (to me) if ALL/which constructs have Myc tags or not - so I have only entered a Myc tag if it's presence is explicitly mentioned. cshatz@stanford.edu,tkim808@stanford.edu 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro electrophoresis electrophoretic mobility-based method electrophoresis western blot western blot Immuno blot Immuno blot Kim T., Vidal GS., Djurisic M., William CM., Birnbaum ME., Garcia KC., Hyman BT., Shatz CJ. For several figs - IK, 2B + more; there is no detection of one component - so interactions not entered. From the "DNA Constructs" section in Supplement it is not clear (to me) if ALL/which constructs have Myc tags or not - so I have only entered a Myc tag if it's presence is explicitly mentioned. cshatz@stanford.edu,tkim808@stanford.edu Accepted 2017-NOV-09 AT 16:22 GMT AT 16:22 GMT by PPORRAS Science (New York, N.Y.) 2013 Only protein-protein interactions imex curation imex curation done; done; done; added; I have not added an "Exp-Prep" for PirB - From my email:-Should "ExpPrep" for PirB (Prey) be "physiological" or "under-expressed" - or both, or left blank? - as it has only one copy of the PirB gene, and we do not know if protein levels would be "normal" or reduced?; done-rat protein in EBI-16416578 is 90% pure according to R+D Datasheet - I presume we enter as "purified" although I do not know what the "cut-off" for the percentage figure might be..; done; I have deleted (EBI-16416521) and added Fig S1C to EBI-16416750) - with Stoichiometry =1 for Pir3. Human LilrB2 is a β-amyloid receptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's model. Human LilrB2 is a β-amyloid receptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's model. Science (New York, N.Y.) 2013 imex curation Kim T., Vidal GS., Djurisic M., William CM., Birnbaum ME., Garcia KC., Hyman BT., Shatz CJ. For several figs - IK, 2B + more; there is no detection of one component - so interactions not entered. From the "DNA Constructs" section in Supplement it is not clear (to me) if ALL/which constructs have Myc tags or not - so I have only entered a Myc tag if it's presence is explicitly mentioned. cshatz@stanford.edu,tkim808@stanford.edu 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging tag visualisation tag visualisation Kim T., Vidal GS., Djurisic M., William CM., Birnbaum ME., Garcia KC., Hyman BT., Shatz CJ. For several figs - IK, 2B + more; there is no detection of one component - so interactions not entered. From the "DNA Constructs" section in Supplement it is not clear (to me) if ALL/which constructs have Myc tags or not - so I have only entered a Myc tag if it's presence is explicitly mentioned. Bound Amyloid-beta-biotin peptides were visualized with streptavidin-alexa fluorophore or Alkaline phosphatase -streptavidin conjugates. cshatz@stanford.edu,tkim808@stanford.edu Accepted 2017-NOV-09 AT 16:22 GMT AT 16:22 GMT by PPORRAS Science (New York, N.Y.) 2013 Only protein-protein interactions imex curation imex curation done; done; done; added; I have not added an "Exp-Prep" for PirB - From my email:-Should "ExpPrep" for PirB (Prey) be "physiological" or "under-expressed" - or both, or left blank? - as it has only one copy of the PirB gene, and we do not know if protein levels would be "normal" or reduced?; done-rat protein in EBI-16416578 is 90% pure according to R+D Datasheet - I presume we enter as "purified" although I do not know what the "cut-off" for the percentage figure might be..; done; I have deleted (EBI-16416521) and added Fig S1C to EBI-16416750) - with Stoichiometry =1 for Pir3. Human LilrB2 is a β-amyloid receptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's model. Human LilrB2 is a β-amyloid receptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's model. Science (New York, N.Y.) 2013 imex curation Kim T., Vidal GS., Djurisic M., William CM., Birnbaum ME., Garcia KC., Hyman BT., Shatz CJ. For several figs - IK, 2B + more; there is no detection of one component - so interactions not entered. From the "DNA Constructs" section in Supplement it is not clear (to me) if ALL/which constructs have Myc tags or not - so I have only entered a Myc tag if it's presence is explicitly mentioned. cshatz@stanford.edu,tkim808@stanford.edu 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Kim T., Vidal GS., Djurisic M., William CM., Birnbaum ME., Garcia KC., Hyman BT., Shatz CJ. For several figs - IK, 2B + more; there is no detection of one component - so interactions not entered. From the "DNA Constructs" section in Supplement it is not clear (to me) if ALL/which constructs have Myc tags or not - so I have only entered a Myc tag if it's presence is explicitly mentioned. cshatz@stanford.edu,tkim808@stanford.edu Accepted 2017-NOV-09 AT 16:22 GMT AT 16:22 GMT by PPORRAS Science (New York, N.Y.) 2013 Only protein-protein interactions imex curation imex curation done; done; done; added; I have not added an "Exp-Prep" for PirB - From my email:-Should "ExpPrep" for PirB (Prey) be "physiological" or "under-expressed" - or both, or left blank? - as it has only one copy of the PirB gene, and we do not know if protein levels would be "normal" or reduced?; done-rat protein in EBI-16416578 is 90% pure according to R+D Datasheet - I presume we enter as "purified" although I do not know what the "cut-off" for the percentage figure might be..; done; I have deleted (EBI-16416521) and added Fig S1C to EBI-16416750) - with Stoichiometry =1 for Pir3. Human LilrB2 is a β-amyloid receptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's model. Human LilrB2 is a β-amyloid receptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's model. Science (New York, N.Y.) 2013 imex curation Kim T., Vidal GS., Djurisic M., William CM., Birnbaum ME., Garcia KC., Hyman BT., Shatz CJ. For several figs - IK, 2B + more; there is no detection of one component - so interactions not entered. From the "DNA Constructs" section in Supplement it is not clear (to me) if ALL/which constructs have Myc tags or not - so I have only entered a Myc tag if it's presence is explicitly mentioned. cshatz@stanford.edu,tkim808@stanford.edu 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim T., Vidal GS., Djurisic M., William CM., Birnbaum ME., Garcia KC., Hyman BT., Shatz CJ. For several figs - IK, 2B + more; there is no detection of one component - so interactions not entered. From the "DNA Constructs" section in Supplement it is not clear (to me) if ALL/which constructs have Myc tags or not - so I have only entered a Myc tag if it's presence is explicitly mentioned. cshatz@stanford.edu,tkim808@stanford.edu Accepted 2017-NOV-09 AT 16:22 GMT AT 16:22 GMT by PPORRAS Science (New York, N.Y.) 2013 Only protein-protein interactions imex curation imex curation done; done; done; added; I have not added an "Exp-Prep" for PirB - From my email:-Should "ExpPrep" for PirB (Prey) be "physiological" or "under-expressed" - or both, or left blank? - as it has only one copy of the PirB gene, and we do not know if protein levels would be "normal" or reduced?; done-rat protein in EBI-16416578 is 90% pure according to R+D Datasheet - I presume we enter as "purified" although I do not know what the "cut-off" for the percentage figure might be..; done; I have deleted (EBI-16416521) and added Fig S1C to EBI-16416750) - with Stoichiometry =1 for Pir3. Human LilrB2 is a β-amyloid receptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's model. Human LilrB2 is a β-amyloid receptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's model. Science (New York, N.Y.) 2013 imex curation Kim T., Vidal GS., Djurisic M., William CM., Birnbaum ME., Garcia KC., Hyman BT., Shatz CJ. For several figs - IK, 2B + more; there is no detection of one component - so interactions not entered. From the "DNA Constructs" section in Supplement it is not clear (to me) if ALL/which constructs have Myc tags or not - so I have only entered a Myc tag if it's presence is explicitly mentioned. cshatz@stanford.edu,tkim808@stanford.edu 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-forebrain Mus musculus, forebrain brain_forebrain anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim T., Vidal GS., Djurisic M., William CM., Birnbaum ME., Garcia KC., Hyman BT., Shatz CJ. Interactions involving antibodies to Cofilin have not been included due to lack of antibody specificity. Interactions with PP2A, B, or C have not been included due to unknown identity of proteins targetted. For several figs - IK, 2B + more; there is no detection of one component - so interactions not entered. From the "DNA Constructs" section in Supplement it is not clear (to me) if ALL/which constructs have Myc tags or not - so I have only entered a Myc tag if it's presence is explicitly mentioned. cshatz@stanford.edu,tkim808@stanford.edu Accepted 2017-NOV-09 AT 16:22 GMT AT 16:22 GMT by PPORRAS Science (New York, N.Y.) 2013 Only protein-protein interactions imex curation imex curation done; done; done; added; I have not added an "Exp-Prep" for PirB - From my email:-Should "ExpPrep" for PirB (Prey) be "physiological" or "under-expressed" - or both, or left blank? - as it has only one copy of the PirB gene, and we do not know if protein levels would be "normal" or reduced?; done-rat protein in EBI-16416578 is 90% pure according to R+D Datasheet - I presume we enter as "purified" although I do not know what the "cut-off" for the percentage figure might be..; done; I have deleted (EBI-16416521) and added Fig S1C to EBI-16416750) - with Stoichiometry =1 for Pir3. Human LilrB2 is a β-amyloid receptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's model. Human LilrB2 is a β-amyloid receptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's model. Science (New York, N.Y.) 2013 imex curation Kim T., Vidal GS., Djurisic M., William CM., Birnbaum ME., Garcia KC., Hyman BT., Shatz CJ. For several figs - IK, 2B + more; there is no detection of one component - so interactions not entered. From the "DNA Constructs" section in Supplement it is not clear (to me) if ALL/which constructs have Myc tags or not - so I have only entered a Myc tag if it's presence is explicitly mentioned. cshatz@stanford.edu,tkim808@stanford.edu 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Kim T., Vidal GS., Djurisic M., William CM., Birnbaum ME., Garcia KC., Hyman BT., Shatz CJ. For several figs - IK, 2B + more; there is no detection of one component - so interactions not entered. From the "DNA Constructs" section in Supplement it is not clear (to me) if ALL/which constructs have Myc tags or not - so I have only entered a Myc tag if it's presence is explicitly mentioned. cshatz@stanford.edu,tkim808@stanford.edu Accepted 2017-NOV-09 AT 16:22 GMT AT 16:22 GMT by PPORRAS Science (New York, N.Y.) 2013 Only protein-protein interactions imex curation imex curation done; done; done; added; I have not added an "Exp-Prep" for PirB - From my email:-Should "ExpPrep" for PirB (Prey) be "physiological" or "under-expressed" - or both, or left blank? - as it has only one copy of the PirB gene, and we do not know if protein levels would be "normal" or reduced?; done-rat protein in EBI-16416578 is 90% pure according to R+D Datasheet - I presume we enter as "purified" although I do not know what the "cut-off" for the percentage figure might be..; done; I have deleted (EBI-16416521) and added Fig S1C to EBI-16416750) - with Stoichiometry =1 for Pir3. Human LilrB2 is a β-amyloid receptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's model. Human LilrB2 is a β-amyloid receptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's model. Science (New York, N.Y.) 2013 imex curation Kim T., Vidal GS., Djurisic M., William CM., Birnbaum ME., Garcia KC., Hyman BT., Shatz CJ. For several figs - IK, 2B + more; there is no detection of one component - so interactions not entered. From the "DNA Constructs" section in Supplement it is not clear (to me) if ALL/which constructs have Myc tags or not - so I have only entered a Myc tag if it's presence is explicitly mentioned. cshatz@stanford.edu,tkim808@stanford.edu 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Kim T., Vidal GS., Djurisic M., William CM., Birnbaum ME., Garcia KC., Hyman BT., Shatz CJ. For several figs - IK, 2B + more; there is no detection of one component - so interactions not entered. From the "DNA Constructs" section in Supplement it is not clear (to me) if ALL/which constructs have Myc tags or not - so I have only entered a Myc tag if it's presence is explicitly mentioned. cshatz@stanford.edu,tkim808@stanford.edu Accepted 2017-NOV-09 AT 16:22 GMT AT 16:22 GMT by PPORRAS Science (New York, N.Y.) 2013 Only protein-protein interactions imex curation imex curation done; done; done; added; I have not added an "Exp-Prep" for PirB - From my email:-Should "ExpPrep" for PirB (Prey) be "physiological" or "under-expressed" - or both, or left blank? - as it has only one copy of the PirB gene, and we do not know if protein levels would be "normal" or reduced?; done-rat protein in EBI-16416578 is 90% pure according to R+D Datasheet - I presume we enter as "purified" although I do not know what the "cut-off" for the percentage figure might be..; done; I have deleted (EBI-16416521) and added Fig S1C to EBI-16416750) - with Stoichiometry =1 for Pir3. Human LilrB2 is a β-amyloid receptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's model. Human LilrB2 is a β-amyloid receptor and its murine homolog PirB regulates synaptic plasticity in an Alzheimer's model. Science (New York, N.Y.) 2013 imex curation Kim T., Vidal GS., Djurisic M., William CM., Birnbaum ME., Garcia KC., Hyman BT., Shatz CJ. For several figs - IK, 2B + more; there is no detection of one component - so interactions not entered. From the "DNA Constructs" section in Supplement it is not clear (to me) if ALL/which constructs have Myc tags or not - so I have only entered a Myc tag if it's presence is explicitly mentioned. cshatz@stanford.edu,tkim808@stanford.edu 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Kim T., Vidal GS., Djurisic M., William CM., Birnbaum ME., Garcia KC., Hyman BT., Shatz CJ. For several figs - IK, 2B + more; there is no detection of one component - so interactions not entered. From the "DNA Constructs" section in Supplement it is not clear (to me) if ALL/which constructs have Myc tags or not - so I have only entered a Myc tag if it's presence is explicitly mentioned. cshatz@stanford.edu,tkim808@stanford.edu Accepted 2017-NOV-09 AT 16:22 GMT AT 16:22 GMT by PPORRAS Science (New York, N.Y.) 2013 Only protein-protein interactions imex curation imex curation done; done; done; added; I have not added an "Exp-Prep" for PirB - From my email:-Should "ExpPrep" for PirB (Prey) be "physiological" or "under-expressed" - or both, or left blank? - as it has only one copy of the PirB gene, and we do not know if protein levels would be "normal" or reduced?; done-rat protein in EBI-16416578 is 90% pure according to R+D Datasheet - I presume we enter as "purified" although I do not know what the "cut-off" for the percentage figure might be..; done; I have deleted (EBI-16416521) and added Fig S1C to EBI-16416750) - with Stoichiometry =1 for Pir3. rtn4_rat Reticulon-4 Rtn4 Nogo Neurite outgrowth inhibitor Foocen Glut4 vesicle 20 kDa protein protein protein rat Rattus norvegicus (Rat) Rat MEDIDQSSLVSSSTDSPPRPPPAFKYQFVTEPEDEEDEEEEEDEEEDDEDLEELEVLERKPAAGLSAAAVPPAAAAPLLDFSSDSVPPAPRGPLPAAPPAAPERQPSWERSPAAPAPSLPPAAAVLPSKLPEDDEPPARPPPPPPAGASPLAEPAAPPSTPAAPKRRGSGSVDETLFALPAASEPVIPSSAEKIMDLMEQPGNTVSSGQEDFPSVLLETAASLPSLSPLSTVSFKEHGYLGNLSAVSSSEGTIEETLNEASKELPERATNPFVNRDLAEFSELEYSEMGSSFKGSPKGESAILVENTKEEVIVRSKDKEDLVCSAALHSPQESPVGKEDRVVSPEKTMDIFNEMQMSVVAPVREEYADFKPFEQAWEVKDTYEGSRDVLAARANVESKVDRKCLEDSLEQKSLGKDSEGRNEDASFPSTPEPVKDSSRAYITCASFTSATESTTANTFPLLEDHTSENKTDEKKIEERKAQIITEKTSPKTSNPFLVAVQDSEADYVTTDTLSKVTEAAVSNMPEGLTPDLVQEACESELNEATGTKIAYETKVDLVQTSEAIQESLYPTAQLCPSFEEAEATPSPVLPDIVMEAPLNSLLPSAGASVVQPSVSPLEAPPPVSYDSIKLEPENPPPYEEAMNVALKALGTKEGIKEPESFNAAVQETEAPYISIACDLIKETKLSTEPSPDFSNYSEIAKFEKSVPEHAELVEDSSPESEPVDLFSDDSIPEVPQTQEEAVMLMKESLTEVSETVAQHKEERLSASPQELGKPYLESFQPNLHSTKDAASNDIPTLTKKEKISLQMEEFNTAIYSNDDLLSSKEDKIKESETFSDSSPIEIIDEFPTFVSAKDDSPKLAKEYTDLEVSDKSEIANIQSGADSLPCLELPCDLSFKNIYPKDEVHVSDEFSENRSSVSKASISPSNVSALEPQTEMGSIVKSKSLTKEAEKKLPSDTEKEDRSLSAVLSAELSKTSVVDLLYWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQAIQKSDEGHPFRAYLESEVAISEELVQKYSNSALGHVNSTIKELRRLFLVDDLVDSLKFAVLMWVFTYVGALFNGLTLLILALISLFSIPVIYERHQVQIDHYLGLANKSVKDAMAKIQAKIPGLKRKAD 8CB894B09E94F0B6 lirb2_human Leukocyte immunoglobulin-like receptor subfamily B member 2 LILRB2 ILT4 LIR2 MIR10 CD85 antigen-like family member D Immunoglobulin-like transcript 4 Monocyte/macrophage immunoglobulin-like receptor 10 protein protein human Homo sapiens Human MTPIVTVLICLGLSLGPRTHVQTGTIPKPTLWAEPDSVITQGSPVTLSCQGSLEAQEYRLYREKKSASWITRIRPELVKNGQFHIPSITWEHTGRYGCQYYSRARWSELSDPLVLVMTGAYPKPTLSAQPSPVVTSGGRVTLQCESQVAFGGFILCKEGEEEHPQCLNSQPHARGSSRAIFSVGPVSPNRRWSHRCYGYDLNSPYVWSSPSDLLELLVPGVSKKPSLSVQPGPVVAPGESLTLQCVSDVGYDRFVLYKEGERDLRQLPGRQPQAGLSQANFTLGPVSRSYGGQYRCYGAHNLSSECSAPSDPLDILITGQIRGTPFISVQPGPTVASGENVTLLCQSWRQFHTFLLTKAGAADAPLRLRSIHEYPKYQAEFPMSPVTSAHAGTYRCYGSLNSDPYLLSHPSEPLELVVSGPSMGSSPPPTGPISTPAGPEDQPLTPTGSDPQSGLGRHLGVVIGILVAVVLLLLLLLLLFLILRHRRQGKHWTSTQRKADFQHPAGAVGPEPTDRGLQWRSSPAADAQEENLYAAVKDTQPEDGVEMDTRAAASEAPQDVTYAQLHSLTLRRKATEPPPSQEREPPAEPSIYATLAIH DIP protein Q8N423 original sequence version: 127 DIP protein Q8N423 original sequence version: 104 33E9737AFCAFCE2D a2ntk1_mouse Pira4 6M7 protein protein mouse Mus musculus Mouse MSCTFTALLCLGLTLSLWIPVLTGSLPKPILRVQPHSVVSRRTKVTFLCEETIGANEYHLYKDGKLYKTVTKNKQKPANKAEFSLSNVDLSNAGQYRCSYNTQDESSGYSDPLELVVTGDYWTPSLSAQASPVVTSGGYVTLQCESWHNNHRFILTVEGPQKLSWMQDSQYNNSTRKYHALFSVGPVTPNQRWIFRCYSYDRNRPYVWSPPSESVELLVSGNLQKPTIKAEPGPVIASKRAMTIWCQGNLDAEVYFLHNEKSQKTQSTQTLQEPGNKGRFFIPSVTQQHAGQYRCYCYSSAGWSQPSDTLELVVTGIYEYYEPRLSVLPSPVVTAGGNMTLHCASDFHYDKFILTKEDKKFTSSLDTEHISSSRQYRALFIIGPTTPTHTGTFRCYGYYKNTPQLWSVPSALQQILISGLSKKPSLLTHQGHILDPGMTLTLQCFSDINYDRYALHKVGGADIMQHSSQQTDTGFSVANFTLGYVRSSTGGQYRCYGAHNLSSEWSASSEPLDILITGQLPLTPSLSVKPNYTVQSGENVSLLCWSMDSVDTFILSKEGSAQQPLRLKSKSHDQQSQAEFSMSAVTSHLSGTYRCYGAQDSSFYLLSSASAPVELTVSETIESSTWSPKRPIPPIPTENKDHTMENLIRMGMAVLVLIVLSILATEAWRSHRQTHPAAGN 22BFC418636034A8 lirb3_mouse Leukocyte immunoglobulin-like receptor subfamily B member 3 Lilrb3 Pirb Cell-surface glycoprotein p91 Paired immunoglobulin-like receptor B protein protein mouse Mus musculus Mouse MSCTFTALLRLGLTLSLWIPVLTGSLPKPILRVQPDSVVSRRTKVTFLCEETIGANEYRLYKDGKLYKTVTKNKQKPENKAEFSFSNVDLSNAGQYRCSYSTQYKSSGYSDLLELVVTGHYWTPSLLAQASPVVTSGGYVTLQCESWHNDHKFILTVEGPQKLSWTQDSQYNYSTRKYHALFSVGPVTPNQRWICRCYSYDRNRPYVWSPPSESVELLVSGNLQKPTIKAEPGSVITSKRAMTIWCQGNLDAEVYFLHNEKSQKTQSTQTLQEPGNKGKFFIPSVTLQHAGQYRCYCYGSAGWSQPSDTLELVVTGIYEYYEPRLSVLPSPVVTAGGNMTLHCASDFPYDKFILTKEDKKFGNSLDTEHISSSGQYRALFIIGPTTPTHTGAFRCYGYYKNAPQLWSVPSALQQILISGLSKKPSLLTHQGHILDPGMTLTLQCFSDINYDRFALHKVGGADIMQHSSQQTDTGFSVANFTLGYVSSSTGGQYRCYGAHNLSSEWSASSEPLDILITGQLPLTPSLSVQPNHTVHSGETVSLLCWSMDSVDTFILSKEGSAQQPLRLKSKSHDQQSQAEFSMSAVTSHLSGTYRCYGAQDSSFYLLSSASAPVELTVSGPIETSTPPPTMSMPLGGLHMYLKALIGVSVAFILFLFIFIFILLRRRHRGKFRKDVQKEKDLQLSSGAEEPITRKGELQKRPNPAAATQEESLYASVEDMQTEDGVELNSWTPPEEDPQGETYAQVKPSRLRKAGHVSPSVMSREQLNTEYEQAEEGQGANNQAAESGESQDVTYAQLCSRTLRQGAAASPLSQAGEAPEEPSVYATLAAARPEAVPKDMEQ DIP protein P97484 original sequence version: 91 DIP protein P97484 original sequence version: 77 ptn11_mouse Tyrosine-protein phosphatase non-receptor type 11 Ptpn11 Protein-tyrosine phosphatase SYP SH-PTP2 protein protein mouse Mus musculus Mouse MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIVDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLVDQTSGDQSPLPPCTPTPPCAEMREDSARVYENVGLMQQQRSFR DIP protein P35235 original sequence version: 93 DIP protein P35235 original sequence version: 99 DIP protein P35235 original sequence version: 138 6C71EEEECCF7F159 p05067-pro_0000000092 Amyloid-beta protein 42 Alzheimer disease amyloid protein Cerebral vascular amyloid peptide APP A4 AD1 ABPP PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 3AC85563D7858C37 lilrb2-app-5 3 14 unspecified role unspecified role prey prey purified purified whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ecd_D1D2_regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 14 unspecified role unspecified role prey prey purified purified whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 22 17 physical association physical association 2 G/H lilrb2-app-6 8 14 unspecified role unspecified role neutral component neutral component purified purified n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 14 unspecified role unspecified role neutral component neutral component purified purified n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 1A-1 Beta-amyloid peptide consists primarily of high-n oligomers. app-117 1 14 unspecified role unspecified role neutral component neutral component purified purified n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 14 unspecified role unspecified role neutral component neutral component purified purified n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 1B, S1B Beta-amyloid peptide consists primarily of high-n oligomers. app-lilrb3-1 2 14 unspecified role unspecified role neutral component neutral component purified purified n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 12 tag visualisation tag visualisation tag visualisation tag visualisation unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 14 unspecified role unspecified role neutral component neutral component purified purified n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 41 36 colocalization colocalization 1 C/D Relative to monomeric Amyloid-beta42, oligomerized peptides bound to PirB-expressing cells about 6 times as much. app-app-67 7 14 unspecified role unspecified role neutral component neutral component purified purified possible_n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 12 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 14 unspecified role unspecified role neutral component neutral component purified purified possible_n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 49 45 colocalization colocalization 1 E Oligomerised Amyloid-beta42 peptides were used. lilrb2-app-7 3 14 unspecified role unspecified role prey prey whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 12 unspecified role unspecified role bait bait ecd_D1D2_regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 14 unspecified role unspecified role prey prey whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 57 52 physical association physical association 2 G/H, Supp 6C 245tw34at-1 5 13 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey under-expressed under expressed level under-expressed physical association physical association 4A app-app-68 4 14 unspecified role unspecified role prey prey purified purified n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 14 unspecified role unspecified role prey prey purified purified n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 68 64 physical association physical association Supp Fig 1B PirB interactspreferentially with oligomeric forms of Amyloid-beta 1-42. app-lilrb3-2 6 12 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 14 unspecified role unspecified role bait bait n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association Supp Fig 1Ci PirB interactspreferentially with oligomeric forms of Amyloid-beta 1-42. lilrb3-app-2 4 12 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 9 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association Supp Fig 2 lilrb3-app-3 4 14 unspecified role unspecified role bait bait purified purified n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 12 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 14 unspecified role unspecified role bait bait purified purified n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 81 85 physical association physical association Supp Fig 3D app-pira4-1 4 14 unspecified role unspecified role bait bait purified purified n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 14 unspecified role unspecified role bait bait purified purified n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 93 89 physical association physical association Supp Fig 3D-2 weak binding app-app-69 7 14 unspecified role unspecified role neutral component neutral component purified purified possible_n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 14 unspecified role unspecified role neutral component neutral component purified purified possible_n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 103 97 colocalization colocalization Supp 5 Oligomerised Amyloid-beta42 peptides were used. app-lilrb3-3 6 14 unspecified role unspecified role bait bait purified purified n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 12 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 14 unspecified role unspecified role bait bait purified purified n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 111 107 physical association physical association Supp fig 6A PirB interactspreferentially with oligomeric forms of Amyloid-beta 1-42. Amyloid-beta 1-42 oligomers appear to bind preferentially to dimeric PirB, as seen under partially-reducing conditions. PirB: there is NO Fc tag here so cannot be FC-Fc dimerisation! app-lilrb3-4 6 14 unspecified role unspecified role bait bait purified purified n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 12 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 14 unspecified role unspecified role bait bait purified purified n_terminus biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 115 119 physical association physical association Supp Fig 1C, Supp fig 6Ai PirB interacts preferentially with oligomeric forms of Amyloid-beta 1-42. This interaction is seen under fully reducing conditions.