DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. PLoS biology 2013 imex curation Beyhan S., Gutierrez M., Voorhies M., Sil A. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Beyhan S., Gutierrez M., Voorhies M., Sil A. DIP original experiment: DIP-254657X PLoS biology 2013 IMEx A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. PLoS biology 2013 imex curation Beyhan S., Gutierrez M., Voorhies M., Sil A. Only protein-protein interactions ajeca Ajellomyces capsulatus Darling's disease fungus Histoplasma capsulatum anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Beyhan S., Gutierrez M., Voorhies M., Sil A. DIP original experiment: DIP-254655X PLoS biology 2013 IMEx A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. PLoS biology 2013 imex curation Beyhan S., Gutierrez M., Voorhies M., Sil A. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Beyhan S., Gutierrez M., Voorhies M., Sil A. DIP original experiment: DIP-254658X PLoS biology 2013 IMEx A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. PLoS biology 2013 imex curation Beyhan S., Gutierrez M., Voorhies M., Sil A. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Beyhan S., Gutierrez M., Voorhies M., Sil A. DIP original experiment: DIP-254656X PLoS biology 2013 IMEx A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. PLoS biology 2013 imex curation Beyhan S., Gutierrez M., Voorhies M., Sil A. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Beyhan S., Gutierrez M., Voorhies M., Sil A. DIP original experiment: DIP-254659X PLoS biology 2013 IMEx A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. PLoS biology 2013 imex curation Beyhan S., Gutierrez M., Voorhies M., Sil A. Only protein-protein interactions ajeca Ajellomyces capsulatus Darling's disease fungus Histoplasma capsulatum anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Beyhan S., Gutierrez M., Voorhies M., Sil A. DIP original experiment: DIP-254654X PLoS biology 2013 IMEx A temperature-responsive network links cell shape and virulence traits in a primary fungal pathogen. a9qxx8_ajeca protein protein ajeca Ajellomyces capsulatus Darling's disease fungus Histoplasma capsulatum MGNGTAAVLEPTFTGYVATTQDALILFEACLTGILHHVPRRPHDRERSQLVKSGSVFIYEENASGIKRWTDGVTWSPSRILGNFLVYRELDKPFPPGEKKRAMKKGNRRPTSTGRPGEPYPRPQESNGTTYSPATSPGAAPYGERNQQSELERQLVGSLVDSYGFKDSGLVKKTMSVTVSGVTHHLVSYYSVEDVMSNRLHTPMQHESLRYIRPRAELTTKQSFRAPIDDFDHATLEDADPTSALYAYRSTLSGAQAYAVPSSGPSYYQLPAYGAPPGYAVGAAGLAGHPASNPYLANTSNSADIPPKQESYPFRSSASYPPGYNPMNPSMQQPLSASTMSSSLNTVLADRSQQQQQPSSAGLYRNPALQPRNVAPDSSSGAVDPSSAYSRGTFGGMDSADQQNVGQHPPYNPATRRESSTLVANVSYYNGDRQPQQQQSQPPSQPQQSHQPPPQQQYFSGPTHSAHASSYQPGPPASMSWNTAATG DIP protein A9QXX8 original sequence version: 8 velb_ajeca Velvet complex subunit RYP3 RYP3 protein protein ajeca Ajellomyces capsulatus Darling's disease fungus Histoplasma capsulatum MYTLKQDRPHPVPPPPPLTMDHLQHGYSTNTPVTLPGIPEAARSVSTVYEGRIYSLDVVQQPIRARMCGFGDKDRRPLTPPPCIRLIVRDATTQKEIDINEIDTSFYVLTVDLWNAEGTSEVNLVRHSATSPSISTATSSAYPPPANIMPFPQYAQTHGYPQTAYPPYYGGQPGYNPHAGYQYPQGGPDPYYPGLPAASTYYPPGTPTQALGPQSALGPHISSASTGMFTRNLIGSLSASAFRLTDPDDKIGVWFVLQDLSVRTEGSFRLKMNFVNVGTQSQAQDQSPGGTPSSPVINVGSAPVLASAFSEVFQVYSAKKFPGVIESTPLSKCFAFQGIKIPIRKDGVKGPRGGQSHGSKGQDGEGEDWENEG DIP protein B2CQK1 original sequence version: 9 vosa_ajeca Spore development regulator RYP2 RYP2 protein protein ajeca Ajellomyces capsulatus Darling's disease fungus Histoplasma capsulatum MSAPTFAEDPVTQRPALQSADFRLTVRQNPERARVAGGKEKERKAVDPPPIIQLKIDESKDPGQNYLQSPFYFMCCTLYNATEDTPATTAQSTALAGTLVSSLHRLKDTDNMEGGFFVFGDLSVKIEGEFRLKFNLFEMREVKRCGGSRDEVVYIKSILSKPFTVLPPKNFPGMTESTWLSRSFADQGVKLRIRKEARTLLKRPLNRAEPDYPAPPTQPRTPERSGASSQQMVAAYQTNRDYPFYNGPDAKRPRSSVDMSGRGLYEGDARYSYPPHAANPYQGQTYQQGMMQGYPTAGAGVSDYAIRQAQQGSTPTPFGSADESIGSIRSPGAGGYIGQPRYQSYSGAQMPYNLASSTQMSQMGESTPGRTGQAANMGGIVSGQSFSQSAGHMQSPSQVPPAWG DIP protein B2CQK0 original sequence version: 9 ryp2-ryp3-1 4 8 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role bait bait N-terminal domain Region 1-208 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association ryp2-a9qxx8_ajeca-1 6 7 unspecified role unspecified role prey prey ajeca Ajellomyces capsulatus Darling's disease fungus Histoplasma capsulatum 8 unspecified role unspecified role prey prey ajeca Ajellomyces capsulatus Darling's disease fungus Histoplasma capsulatum 9 unspecified role unspecified role bait bait ajeca Ajellomyces capsulatus Darling's disease fungus Histoplasma capsulatum association association ryp2-ryp2-1 5 9 unspecified role unspecified role prey prey C-terminal domain Region 199-404 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association ryp3-a9qxx8_ajeca-1 2 7 unspecified role unspecified role prey prey ajeca Ajellomyces capsulatus Darling's disease fungus Histoplasma capsulatum 8 unspecified role unspecified role bait bait ajeca Ajellomyces capsulatus Darling's disease fungus Histoplasma capsulatum 9 unspecified role unspecified role prey prey ajeca Ajellomyces capsulatus Darling's disease fungus Histoplasma capsulatum association association ryp3-ryp2-1 1 8 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role prey prey N-terminal domain Region 1-208 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association ryp2-ryp2-2 3 9 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role bait bait C-terminal domain Region 199-404 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association