DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Nature 2013 imex curation Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. DIP original experiment: DIP-254597X Nature 2013 IMEx Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Nature 2013 imex curation Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. DIP original experiment: DIP-254598X Nature 2013 IMEx Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Nature 2013 imex curation Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. DIP original experiment: DIP-254602X Nature 2013 IMEx Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Nature 2013 imex curation Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. DIP original experiment: DIP-254600X Nature 2013 IMEx Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Nature 2013 imex curation Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. DIP original experiment: DIP-254601X Nature 2013 IMEx Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Nature 2013 imex curation Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. DIP original experiment: DIP-254599X Nature 2013 IMEx Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. braf_human Serine/threonine-protein kinase B-raf BRAF BRAF1 RAFB1 v-Raf murine sarcoma viral oncogene homolog B1 p94 Proto-oncogene B-Raf protein protein human Homo sapiens Human MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH DIP protein P15056 original sequence version: 182 DIP protein P15056 original sequence version: 133 DIP protein P15056 original sequence version: 147 DIP protein P15056 original sequence version: 173 DIP protein P15056 original sequence version: 178 DIP protein P15056 original sequence version: 188 DIP protein P15056 original sequence version: 193 DIP protein P15056 original sequence version: 195 DIP protein P15056 original sequence version: 146 DIP protein P15056 original sequence version: 153 0798C2AAB487E813 raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF DIP protein P04049 original sequence version: 117 DIP protein P04049 original sequence version: 106 DIP protein P04049 original sequence version: 114 DIP protein P04049 original sequence version: 141 DIP protein P04049 original sequence version: 142 DIP protein P04049 original sequence version: 147 DIP protein P04049-1 original sequence version: 136 DIP protein P04049 original sequence version: 136 DIP protein P04049 original sequence version: 153 DIP protein P04049 original sequence version: 150 DIP protein P04049 original sequence version: 164 DIP protein P04049 original sequence version: 172 DIP protein P04049-1 original sequence version: 172 DIP protein P04049 original sequence version: 184 DIP protein P04049 original sequence version: 188 DIP protein P04049 original sequence version: 195 EF821B5349711BC3 mp2k1_human Dual specificity mitogen-activated protein kinase kinase 1 ERK activator kinase 1 MAPK/ERK kinase 1 MAP2K1 MEK1 PRKMK1 protein protein human Homo sapiens Human MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAGV DIP protein Q02750 original sequence version: 96 DIP protein Q02750 original sequence version: 93 DIP protein Q02750-1 original sequence version: 151 DIP protein Q02750 original sequence version: 151 DIP protein Q02750-1 original sequence version: 166 0344118FFC842D51 map2k1-braf-28 3 7 unspecified role unspecified role prey prey Kinase domain Region 416-766 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction map2k1-braf-29 1 7 unspecified role unspecified role prey prey human Homo sapiens Human 8 unspecified role unspecified role prey prey human Homo sapiens Human 9 unspecified role unspecified role bait bait human Homo sapiens Human association association raf1-braf-22 4 7 unspecified role unspecified role prey prey human Homo sapiens Human 8 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association map2k1-braf-30 2 7 unspecified role unspecified role prey prey human Homo sapiens Human 9 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association map2k1-raf1-1 5 8 unspecified role unspecified role prey prey Kinase domain Region 306-648 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction braf-raf1-31 6 7 unspecified role unspecified role bait bait human Homo sapiens Human 8 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association