DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Dimeric WH2 domains in Vibrio VopF promote actin filament barbed-end uncapping and assisted elongation. Dimeric WH2 domains in Vibrio VopF promote actin filament barbed-end uncapping and assisted elongation. Nature structural & molecular biology 2013 imex curation Pernier J., Orban J., Avvaru BS., Jégou A., Romet-Lemonne G., Guichard B., Carlier MF. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Pernier J., Orban J., Avvaru BS., Jégou A., Romet-Lemonne G., Guichard B., Carlier MF. DIP original experiment: DIP-255633X Nature structural & molecular biology 2013 IMEx Dimeric WH2 domains in Vibrio VopF promote actin filament barbed-end uncapping and assisted elongation. Dimeric WH2 domains in Vibrio VopF promote actin filament barbed-end uncapping and assisted elongation. Dimeric WH2 domains in Vibrio VopF promote actin filament barbed-end uncapping and assisted elongation. Nature structural & molecular biology 2013 imex curation Pernier J., Orban J., Avvaru BS., Jégou A., Romet-Lemonne G., Guichard B., Carlier MF. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Pernier J., Orban J., Avvaru BS., Jégou A., Romet-Lemonne G., Guichard B., Carlier MF. DIP original experiment: DIP-255635X Nature structural & molecular biology 2013 IMEx Dimeric WH2 domains in Vibrio VopF promote actin filament barbed-end uncapping and assisted elongation. Dimeric WH2 domains in Vibrio VopF promote actin filament barbed-end uncapping and assisted elongation. Dimeric WH2 domains in Vibrio VopF promote actin filament barbed-end uncapping and assisted elongation. Nature structural & molecular biology 2013 imex curation Pernier J., Orban J., Avvaru BS., Jégou A., Romet-Lemonne G., Guichard B., Carlier MF. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Pernier J., Orban J., Avvaru BS., Jégou A., Romet-Lemonne G., Guichard B., Carlier MF. DIP original experiment: DIP-255636X Nature structural & molecular biology 2013 IMEx Dimeric WH2 domains in Vibrio VopF promote actin filament barbed-end uncapping and assisted elongation. Dimeric WH2 domains in Vibrio VopF promote actin filament barbed-end uncapping and assisted elongation. Dimeric WH2 domains in Vibrio VopF promote actin filament barbed-end uncapping and assisted elongation. Nature structural & molecular biology 2013 imex curation Pernier J., Orban J., Avvaru BS., Jégou A., Romet-Lemonne G., Guichard B., Carlier MF. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Pernier J., Orban J., Avvaru BS., Jégou A., Romet-Lemonne G., Guichard B., Carlier MF. DIP original experiment: DIP-255634X Nature structural & molecular biology 2013 IMEx Dimeric WH2 domains in Vibrio VopF promote actin filament barbed-end uncapping and assisted elongation. c5izn1_vibcl protein protein vibcl vibcl MFKISVSQQANVMSTSDTAQRSSLKISIKSICNKSLSKKLHTLAEKCRRFSQELKEHTASKKQIVEQATTTVRESSLTKSDSELGSSRSLLTSDVLSSSSSHEDLTAVNLEDNDSVFVTIESSSELIVKQDGSIPPAPPLPGNIPPAPPLPSAGNIPTAPGLPKQKATTESVAQTSDNRSKLMEEIRQGVKLRATPKSSSTEKSASDPHSKLMKELINHGAKLKKVSTSDIPVPPPLPAAFASKPTDGRSALLSEIAGFSKDRLRKAGSSETLNVSQPTVAESSIPEAYDLLLSDEMFNLSPKLSETELNTLADSLADYLFKAADIDWMQVIAEQTKGSTQATSLKSQLEQAPEYVKAFCDEILKFPDCYKSADVASPESPKAGPSSVIDVALKRLQAGRNRLFSTIDAKGTNELKKGEAILESAINAARSVMTAEQKSALLSSNVKSATFKVFSELPCMEGFAEQNGKAAFNALRLAFYSSIQSGDTAQQDIARFMKENLATGFSGYSYLGLTSRVAQLEAQLAALTTK DIP protein C5IZN1 original sequence version: 8 acts_rabit Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF DIP protein P68135 original sequence version: 41 DIP protein P68135 original sequence version: 27 DIP protein P68135 original sequence version: 28 DIP protein P68135 original sequence version: 96 DIP protein P68135 original sequence version: 29 DIP protein P68135 original sequence version: 89 DIP protein P68135 original sequence version: 39 DIP protein P68135 original sequence version: 42 DIP protein P68135 original sequence version: 59 DIP protein P68135 original sequence version: 55 DIP protein P68135 original sequence version: 60 DIP protein P68135 original sequence version: 65 DIP protein P68135 original sequence version: 67 DIP protein P68135 original sequence version: 63 DIP protein P68135 original sequence version: 68 DIP protein P68135 original sequence version: 79 DIP protein P68135 original sequence version: 88 DIP protein P68135 original sequence version: 84 DIP protein P68135 original sequence version: 101 DIP protein P68135 original sequence version: 94 DIP protein P68135 original sequence version: 95 DIP protein P68135 original sequence version: 97 DIP protein P68135 original sequence version: 100 DIP protein P68135 original sequence version: 102 DIP protein P68135 original sequence version: 83 DF2A3A046346A179 c5izn1_vibcl-1 1 5 unspecified role unspecified role unspecified role unspecified role Region 283-530 Region 283-530 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 129-506 Region 129-506 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction acta1-c5izn1_vibcl-1 4 6 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit 5 unspecified role unspecified role unspecified role unspecified role Region 246-530 Region 246-530 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 174-286 Region 174-286 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction acta1-c5izn1_vibcl-2 3 6 unspecified role unspecified role unspecified role unspecified role IAEDANS-label IAEDANS-label iaedans label iaedans label IAEDANS 1,5-IAEDANS (5((((2-iodoacetyl)amino)ethyl)amino)-naphthalene-1-sulfonic acid) IAEDANS 1,5-IAEDANS undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit Oryctolagus cuniculus (Rabbit) Rabbit 5 unspecified role unspecified role unspecified role unspecified role Region 246-530 Region 246-530 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 206-286 Region 206-286 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction acta1-c5izn1_vibcl-3 2 6 unspecified role unspecified role unspecified role unspecified role IAEDANS-label IAEDANS-label iaedans label iaedans label IAEDANS 1,5-IAEDANS (5((((2-iodoacetyl)amino)ethyl)amino)-naphthalene-1-sulfonic acid) IAEDANS 1,5-IAEDANS undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit Oryctolagus cuniculus (Rabbit) Rabbit 5 unspecified role unspecified role unspecified role unspecified role Region 174-207 Region 174-207 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 206-249 Region 206-249 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 244-290 Region 244-290 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction