DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for the inhibition of Polo-like kinase 1. Structural basis for the inhibition of Polo-like kinase 1. Nature structural & molecular biology 2013 imex curation Xu J., Shen C., Wang T., Quan J. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Xu J., Shen C., Wang T., Quan J. DIP original experiment: DIP-255629X Nature structural & molecular biology 2013 IMEx Structural basis for the inhibition of Polo-like kinase 1. Structural basis for the inhibition of Polo-like kinase 1. Structural basis for the inhibition of Polo-like kinase 1. Nature structural & molecular biology 2013 imex curation Xu J., Shen C., Wang T., Quan J. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Xu J., Shen C., Wang T., Quan J. DIP original experiment: DIP-255630X Nature structural & molecular biology 2013 IMEx Structural basis for the inhibition of Polo-like kinase 1. Structural basis for the inhibition of Polo-like kinase 1. Structural basis for the inhibition of Polo-like kinase 1. Nature structural & molecular biology 2013 imex curation Xu J., Shen C., Wang T., Quan J. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Xu J., Shen C., Wang T., Quan J. DIP original experiment: DIP-255628X Nature structural & molecular biology 2013 IMEx Structural basis for the inhibition of Polo-like kinase 1. q4kmi8_danre Serine/threonine-protein kinase PLK plk1 Polo-like kinase protein protein danre Danio rerio (Zebrafish) Brachydanio rerio Zebrafish MSAAIAKPSAKPSAHVDPKSAPLKEIPDVLVDPRTMKRYMRGRFLGKGGFAKCYEITDMDTKEVFAGKVVPKSMLLKPHQKEKMSTEIAIHKSLDNPHVVGFHGFFEDDDFVYVVLEICRRRSLLELHKRRKAVTEPEARYFMRQTIQGVQYLHNNRVIHRDLKLGNLFLNDDMDVKIGDFGLATKIEFDGERKKTLCGTPNYIAPEVLCKKGHSFEVDIWSLGCILYTLLVGKPPFETSCLKETYIRIKKNEYSVPRHINPVASALIRRMLHADPTLRPSVAELLTDEFFTSGYAPMRLPTSCLTVPPRFSIAPSSLDPALLRKPLSSLNKGTDSPIEEMGKMDQPQREDLQQRDGPEQPDTHLTDMLQQLAVVNAAKPSDRDFIRQEEAEDPACIPVFWISKWVDYSDKYGLGYQLSDNSVGVLFNDSTRLIMCADGDSLQYIDRNSLESYLSVRSYPSALSKKITLLKYFRNYMSEHLLKAGANITPREGDELTRLPYLRHWFRTKSAIVLHLSNGTVQINFFQDHTKLILCPLMGAVTYINEKREFYTYKMTLIEEFGCCKELASRLRYARNMVEKLMACKSSTTAATSAR DIP protein Q4KMI8 original sequence version: 74 ma205_drome 205 kDa microtubule-associated protein Map205 CG1483 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MEHHEDNAQLDNYLQNRLAESLQICGGAGEHNPHLADATGGNGCAPGIAPSKSDEVDGEEDEEWKYIHEVRQSEKLQQEKLPLTKETGNGFGPGRDSDNQVHGNGAAAVFNLYEEDVEVIKNDGDFSTNSNTTTSTDEVVARQAQEPNQLPEQLQQQQQIESQGVHEDPRQEDEDEHSSVATTYGTSSLSENNSSPLDQEEVVMVAQTVGQEQLVDFDNKENSYFVKNLEENHSQLNPNAVAFVPGVGSQSSSPLPAAEDPLPGVQPRPFLPGGTLDDLVAESPRKEFARINMDGIAVPDEREFDIEADMRPHELEQESDTFGAGHLEMQLLNGIGTADQAALRDVLDHGPETSVDMELPLDQVPNDADIMKQSIYAEHNSSIEDILNSVQPLPIQTCDDKELIHVEEKEHVSKSPSTEELQFQSDFPNNQESHTLFNNTEQDPMQASFYLEHTSQKAQEGCQEQMQLPAECSDIFADQSLLLDTSAPQLSSEADSPVAKLELESQQAGIVDITPSPLSSTAEKHLVEDTKELVEEYTLDPESHFFGVVSSQAPLQLFGKHTLPSIIHSCKHRVASEQNDEENAVFESVSGYETQNFDEISSPPEGINPFAQPFTPAHLVIEQANTMMEDVGGMPIPASEDFAICDKVASKSSNEVEDHRSEQQAFVKEELLHPVGDVVAQVENLGTEKNFVVEEERLPISVSDEIPLSSASKEKLLPDTTDEQLLTSALEEKLRSVAPEESVSTAADGQSISQFDEYVIASNKPLEDILEPEKDVEVAKSLSEKTSVATVAGGAVVGATKTHSATKAGSTAASAKSKTETLVMKKTTASSTSVYGANKSAAPRPSTARLGIKSTSIATKTSTTSSLTGNPRKSLSSNVGSTVKPPTKLSGTRPATAPVSKVTLGAKTITNKPTASGTASDNVTRTTLRPLVSTNARRPATSGTGSVASSTARRPVTNAKGSAPGSAASTKVRPAATMTAPVKPKVLSPRSTISSTTTVRKVPSTSTPSFSTRSPNKQQSNGLGKNTSSTTTSTATATITKSFTARSAPKFTHSASLTYNNGSTSRRLLVPGSSSTTTTSSLRKSSPLKAAPGKAASKPLTPQSKDGTAKSSPAVLKARNSTLMGGEGVAPSNECVPTPNGQINAEEVVKLKGKGLAEEVKIIEEQKLHEQDVPAHNAEVPLLDF DIP protein P23226 original sequence version: 106 47B422E2CEE03F70 plk1-plk1-1 2 4 unspecified role unspecified role bait bait Region 359-595 Region 359-595 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 4 unspecified role unspecified role prey prey Region 17-312 Region 17-312 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q4KMI8:p.Ser123Asp S123D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain S D This feature has been corrected as a result of our quality control procedures. The original label was 'S123D'. Wed May 02 16:07:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser123asp' Tue Jun 30 20:05:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser123Asp' Q4KMI8:p.Arg130Glu R130E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'R130E'. Wed May 02 15:35:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg130glu' Tue Jun 30 19:35:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg130Glu' Q4KMI8:p.Thr306Lys T306K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain T K This feature has been corrected as a result of our quality control procedures. The original label was 'T306K'. Wed May 02 15:19:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr306lys' Tue Jun 30 18:35:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr306Lys' Q4KMI8:p.Val307Arg V307R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V R This feature has been corrected as a result of our quality control procedures. The original label was 'V307R'. Wed May 02 20:02:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val307arg' Tue Jun 30 19:42:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val307Arg' ecolx Escherichia coli putative self interaction putative self interaction map205-plk1-1 3 5 unspecified role unspecified role unspecified role unspecified role Region 276-325 Region 276-325 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role Region 17-312/360-595 Region 17-312/360-595 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction map205-plk1-2 1 5 unspecified role unspecified role unspecified role unspecified role Region 276-325 Region 276-325 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role Region 17-312/360-595 Region 17-312/360-595 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction