DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. Nature chemical biology 2013 imex curation Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. DIP original experiment: DIP-266872X Nature chemical biology 2013 IMEx The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. Nature chemical biology 2013 imex curation Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. DIP original experiment: DIP-266875X Nature chemical biology 2013 IMEx The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. Nature chemical biology 2013 imex curation Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. DIP original experiment: DIP-266874X Nature chemical biology 2013 IMEx The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. Nature chemical biology 2013 imex curation Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. DIP original experiment: DIP-266876X Nature chemical biology 2013 IMEx The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. Nature chemical biology 2013 imex curation Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. DIP original experiment: DIP-266873X Nature chemical biology 2013 IMEx The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. Nature chemical biology 2013 imex curation Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. DIP original experiment: DIP-266877X Nature chemical biology 2013 IMEx The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. ap1g2_human AP-1 complex subunit gamma-like 2 AP1G2 Gamma2-adaptin protein protein human Homo sapiens Human MVVPSLKLQDLIEEIRGAKTQAQEREVIQKECAHIRASFRDGDPVHRHRQLAKLLYVHMLGYPAHFGQMECLKLIASSRFTDKRVGYLGAMLLLDERHDAHLLITNSIKNDLSQGIQPVQGLALCTLSTMGSAEMCRDLAPEVEKLLLQPSPYVRKKAILTAVHMIRKVPELSSVFLPPCAQLLHERHHGILLGTITLITELCERSPAALRHFRKVVPQLVHILRTLVTMGYSTEHSISGVSDPFLQVQILRLLRILGRNHEESSETMNDLLAQVATNTDTSRNAGNAVLFETVLTIMDIRSAAGLRVLAVNILGRFLLNSDRNIRYVALTSLLRLVQSDHSAVQRHRPTVVECLRETDASLSRRALELSLALVNSSNVRAMMQELQAFLESCPPDLRADCASGILLAAERFAPTKRWHIDTILHVLTTAGTHVRDDAVANLTQLIGGAQELHAYSVRRLYNALAEDISQQPLVQVAAWCIGEYGDLLLAGNCEEIEPLQVDEEEVLALLEKVLQSHMSLPATRGYALTALMKLSTRLCGDNNRIRQVVSIYGSCLDVELQQRAVEYDTLFRKYDHMRAAILEKMPLVERDGPQADEEAKESKEAAQLSEAAPVPTEPQASQLLDLLDLLDGASGDVQHPPHLDPSPGGALVHLLDLPCVPPPPAPIPDLKVFEREGVQLNLSFIRPPENPALLLITITATNFSEGDVTHFICQAAVPKSLQLQLQAPSGNTVPARGGLPITQLFRILNPNKAPLRLKLRLTYDHFHQSVQEIFEVNNLPVESWQ DIP protein O75843 original sequence version: 137 C189192C9811111C p03139-1 Large envelope protein S L glycoprotein L-HBsAg Large S protein Large surface protein Major surface antigen L-HBsAg LHB Large envelope protein protein protein hbvd1 Hepatitis B virus genotype D subtype adw (isolate United Kingdom/adyw/1979) HBV-D MGQNLSTSNPLGFFPDHQLDPAFRANTNNPDWDFNPNKDTWPDANKVGAGAFGLGFTPPHGGLLGWSPQAQGIMQTLPANPPPASTNRQSGRQPTPLSPPLRTTHPQAMHWNSTTFHQTLQDPRVRGLYFPAGGSSSGTVNPVPTTTSPISSIFSRIGDPALNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGTTVCLGQNSQSPISNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCPLIPGSSTTSTGSCRTCTTPAQGISMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLWEWASARFSWLSLLVPFVQWFVGLSPIVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCLWAYI DIP protein P03139-1 original sequence version: 71 ap1g2-s-1 1 8 unspecified role unspecified role unspecified role unspecified role Region 1-108 Region 1-108 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 665-785 Region 665-785 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ap1g2-s-2 2 8 unspecified role unspecified role unspecified role unspecified role Region 9-16 Region 9-16 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 29-36 Region 29-36 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 59-68 Region 59-68 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 7 unspecified role unspecified role unspecified role unspecified role Region 665-785 Region 665-785 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ap1g2-s-3 5 8 unspecified role unspecified role unspecified role unspecified role Region 1-108 Region 1-108 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 665-785 Region 665-785 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O75843:p.Ala716Gly A716G mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain A G This feature has been corrected as a result of our quality control procedures. The original label was 'A716G'. Wed May 02 18:05:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala716gly' Tue Jun 30 20:07:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala716Gly' O75843:p.Lys719Gln K719Q mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K Q This feature has been corrected as a result of our quality control procedures. The original label was 'K719Q'. Wed May 02 19:24:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys719gln' Tue Jun 30 16:25:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys719Gln' O75843:p.Ser720Ala S720A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S720A'. Wed May 02 17:00:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser720ala' Tue Jun 30 18:22:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser720Ala' O75843:p.Pro754Ala P754A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain P A This feature has been corrected as a result of our quality control procedures. The original label was 'P754A'. Wed May 02 17:16:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro754ala' Tue Jun 30 16:03:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro754Ala' O75843:p.Arg756Ala R756A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R756A'. Wed May 02 18:35:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg756ala' Tue Jun 30 15:51:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg756Ala' O75843:p.Arg756Gln R756Q mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R Q This feature has been corrected as a result of our quality control procedures. The original label was 'R756Q'. Wed May 02 15:03:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg756gln' Tue Jun 30 17:57:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg756Gln' O75843:p.Arg760Ala R760A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R760A'. Wed May 02 16:56:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg760ala' Tue Jun 30 17:35:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg760Ala' ecolx Escherichia coli direct interaction direct interaction ap1g2-s-4 3 8 unspecified role unspecified role unspecified role unspecified role Region 42-108 Region 42-108 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P03139-1:p.Asp31Ala D31A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D31A'. Wed May 02 15:19:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp31ala' Tue Jun 30 16:38:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp31Ala' P03139-1:p.Trp32Ala W32A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W32A'. Wed May 02 15:52:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp32ala' Tue Jun 30 18:31:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp32Ala' P03139-1:p.Asp33Ala D33A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D33A'. Wed May 02 16:53:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp33ala' Tue Jun 30 18:25:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp33Ala' P03139-1:p.Phe34Ala F34A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F34A'. Wed May 02 16:07:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe34ala' Tue Jun 30 17:17:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe34Ala' ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 665-785 Region 665-785 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ap1g2-s-5 6 8 unspecified role unspecified role unspecified role unspecified role Region 29-36 Region 29-36 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 7 unspecified role unspecified role unspecified role unspecified role Region 665-785 Region 665-785 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ap1g2-s-6 4 8 unspecified role unspecified role unspecified role unspecified role Region 9-16 Region 9-16 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 29-36 Region 29-36 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 59-68 Region 59-68 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 7 unspecified role unspecified role unspecified role unspecified role Region 665-785 Region 665-785 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction