DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Nature structural & molecular biology 2013 imex curation Pan D., Nakatsu T., Kato H. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Pan D., Nakatsu T., Kato H. DIP original experiment: DIP-254747X Nature structural & molecular biology 2013 IMEx Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Nature structural & molecular biology 2013 imex curation Pan D., Nakatsu T., Kato H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Pan D., Nakatsu T., Kato H. DIP original experiment: DIP-254741X Nature structural & molecular biology 2013 IMEx Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Nature structural & molecular biology 2013 imex curation Pan D., Nakatsu T., Kato H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Pan D., Nakatsu T., Kato H. DIP original experiment: DIP-254742X Nature structural & molecular biology 2013 IMEx Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Nature structural & molecular biology 2013 imex curation Pan D., Nakatsu T., Kato H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Pan D., Nakatsu T., Kato H. DIP original experiment: DIP-254744X Nature structural & molecular biology 2013 IMEx Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Nature structural & molecular biology 2013 imex curation Pan D., Nakatsu T., Kato H. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Pan D., Nakatsu T., Kato H. DIP original experiment: DIP-254740X Nature structural & molecular biology 2013 IMEx Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Nature structural & molecular biology 2013 imex curation Pan D., Nakatsu T., Kato H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Pan D., Nakatsu T., Kato H. DIP original experiment: DIP-254743X Nature structural & molecular biology 2013 IMEx Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Nature structural & molecular biology 2013 imex curation Pan D., Nakatsu T., Kato H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Pan D., Nakatsu T., Kato H. DIP original experiment: DIP-254745X Nature structural & molecular biology 2013 IMEx Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Nature structural & molecular biology 2013 imex curation Pan D., Nakatsu T., Kato H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Pan D., Nakatsu T., Kato H. DIP original experiment: DIP-254746X Nature structural & molecular biology 2013 IMEx Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. pex7_yeast Peroxisomal targeting signal 2 receptor PEX7 PAS7 PEB1 YDR142C YD2943.01C YD9302.18C Peroxin-7 Peroxisome import protein PAS7 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLRYHMQGFSGYGVQYSPFFDNRLAVAAGSNFGLVGNGKLFILEIDRSGRIVEVNSFLTQDCLFDLAWNESHENQVLVAQGDGTLRLFDTTFKEFPIAIFKEHEREVFSCNWNLVNRQNFLSSSWDGSIKIWSPLRKQSLMTLTPRPLEITKMVDPLNAIILKKKSFTGISKNRNCVYQAQFSPHDQNLVLSCSGNSYASLFDIRLPSGKNQNNFLVHSGLEALTCDFNKYRPYVVATGGVDNAIRIWDIRMLNKNESATIKRTVPGQLHNSSCINEIPNAHGLAIRKVTWSPHHSNILMSASYDMTCRIWRDLSNDGAKETYKTNSTDATKGSIFNFTQHSEFVFGADWSLWGKPGYVASTAWDGNLFVWNGLG 7D53A42A8ACF23F9 7D53A42A8ACF23F9 thik_yeast 3-ketoacyl-CoA thiolase, peroxisomal POT1 FOX3 POX3 YIL160C Beta-ketothiolase Acetyl-CoA acyltransferase Peroxisomal 3-oxoacyl-CoA thiolase protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQRLQSIKDHLVESAMGKGESKRKNSLLEKRPEDVVIVAANRSAIGKGFKGAFKDVNTDYLLYNFLNEFIGRFPEPLRADLNLIEEVACGNVLNVGAGATEHRAACLASGIPYSTPFVALNRQCSSGLTAVNDIANKIKVGQIDIGLALGVESMTNNYKNVNPLGMISSEELQKNREAKKCLIPMGITNENVAANFKISRKDQDEFAANSYQKAYKAKNEGLFEDEILPIKLPDGSICQSDEGPRPNVTAESLSSIRPAFIKDRGTTTAGNASQVSDGVAGVLLARRSVANQLNLPVLGRYIDFQTVGVPPEIMGVGPAYAIPKVLEATGLQVQDIDIFEINEAFAAQALYCIHKLGIDLNKVNPRGGAIALGHPLGCTGARQVATILRELKKDQIGVVSMCIGTGMGAAAIFIKE B22AA06130829DE5 B22AA06130829DE5 pex21_yeast Peroxisomal membrane protein PEX21 YGR239C G8593 Peroxin-21 PEX21 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPSVCHTSPIEKIIQQGHRIQNDSLIPSKRTKLAHTELTAHYATEDSHVEKHFLHNGSNFDGIDNVRYQNQPSPLTFITPNNTVDSSDWVPQFSSMKIDDSLEFSSEYKRLYSNYESQQRLNSSRQHLPFKNCMIRKTSCTYPPQKTLRQQRQGNRDNPTDAFQFDAEFQVLEREIQKERYEPITRRDEKWFDQDQSELQRIATDIVKCCTPPPSSASSSSTLSSSVESKLSESKFIQLMRNISSGDVTLKKNADGNSASELFSSNNGELVGNRHIFVKDEIHKDILD 0197257317ADE4FF 0197257317ADE4FF fox3p-pex21p-1 5 10 unspecified role unspecified role unspecified role unspecified role Region 1-15 Region 1-15 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Region 190-288 Region 190-288 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction pex21p-pex7p-1 7 11 unspecified role unspecified role bait bait Region 190-288 Region 190-288 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 9 unspecified role unspecified role prey prey picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction fox3p-pex21p-2 1 10 unspecified role unspecified role unspecified role unspecified role Region 1-15 Region 1-15 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Region 190-288 Region 190-288 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain picpa Pichia pastoris (Yeast) Yeast Pichia pastoris physical association physical association pex21p-fox3p-1 4 10 unspecified role unspecified role prey prey ecolx Escherichia coli 11 unspecified role unspecified role bait bait Region 190-288 Region 190-288 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P50091:p.Phe236Asp F236D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F D This feature has been corrected as a result of our quality control procedures. The original label was 'F236D'. Wed May 02 17:39:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe236asp' Tue Jun 30 19:48:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe236Asp' ecolx Escherichia coli 9 unspecified role unspecified role prey prey picpa Pichia pastoris (Yeast) Yeast Pichia pastoris association association pex7p-pex21p-1 3 11 unspecified role unspecified role prey prey Region 190-288 Region 190-288 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction pex21p-fox3p-2 8 10 unspecified role unspecified role prey prey ecolx Escherichia coli 11 unspecified role unspecified role bait bait Region 190-288 Region 190-288 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 9 unspecified role unspecified role prey prey P39108:p.Leu34Asp L34D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L D This feature has been corrected as a result of our quality control procedures. The original label was 'L34D'. Wed May 02 16:38:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu34asp' Tue Jun 30 16:02:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu34Asp' P39108:p.[Phe32Ala;Leu34Ala] F32A/L34A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F32A/L34A'. Wed May 02 15:07:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe32ala,leu34ala' Tue Jun 30 18:06:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe32Ala;Leu34Ala]' P39108:p.[Phe344Ala;Trp364Ala] F344A/W364A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F344A/W364A'. Wed May 02 16:17:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe344ala,trp364ala' Tue Jun 30 17:27:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe344Ala;Trp364Ala]' picpa Pichia pastoris (Yeast) Yeast Pichia pastoris association association pex7p-fox3p-1 2 10 unspecified role unspecified role prey prey ecolx Escherichia coli 11 unspecified role unspecified role prey prey Region 190-288 Region 190-288 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range picpa Pichia pastoris (Yeast) Yeast Pichia pastoris association association pex7p-fox3p-2 6 10 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction