DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Nature structural & molecular biology 2013 imex curation Li Z., Hartl FU., Bracher A. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Li Z., Hartl FU., Bracher A. DIP original experiment: DIP-254736X Nature structural & molecular biology 2013 IMEx Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Nature structural & molecular biology 2013 imex curation Li Z., Hartl FU., Bracher A. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Li Z., Hartl FU., Bracher A. DIP original experiment: DIP-254732X Nature structural & molecular biology 2013 IMEx Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Nature structural & molecular biology 2013 imex curation Li Z., Hartl FU., Bracher A. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Li Z., Hartl FU., Bracher A. DIP original experiment: DIP-254735X Nature structural & molecular biology 2013 IMEx Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Nature structural & molecular biology 2013 imex curation Li Z., Hartl FU., Bracher A. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Li Z., Hartl FU., Bracher A. DIP original experiment: DIP-254734X Nature structural & molecular biology 2013 IMEx Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. Nature structural & molecular biology 2013 imex curation Li Z., Hartl FU., Bracher A. Only protein-protein interactions ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Li Z., Hartl FU., Bracher A. DIP original experiment: DIP-254733X Nature structural & molecular biology 2013 IMEx Structure and function of Hip, an attenuator of the Hsp70 chaperone cycle. hspa1a_hspa1b_human-1 HSPA1A_HSPA1B_human molecule set molecule set human Homo sapiens Human MAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDKPKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD P0DMV8,P0DMV9 f10a1_rat Hsc70-interacting protein Protein ST13 homolog Protein FAM10A1 St13 Fam10a1 Hip protein protein rat Rattus norvegicus (Rat) Rat MDPRKVSELRAFVKMCRQDPSVLHTEEMRFLREWVESMGGKVPPATHKAKSEENTKEEKRDKTTEDNIKTEEPSSEESDLEIDNEGVIEADTDAPQEMGDENAEITEAMMDEANEKKGAAIDALNDGELQKAIDLFTDAIKLNPRLAILYAKRASVFVKLQKPNAAIRDCDRAIEINPDSAQPYKWRGKAHRLLGHWEEAARDLALACKLDYDEDASAMLREVQPRAQKIAEHRRKYERKREEREIKERIERVKKAREEHEKAQREEEARRQSGSQFGSFPGGFPGGMPGNFPGGMPGMGGAMPGMAGMPGLNEILSDPEVLAAMQDPEVMVAFQDVAQNPSNMSKYQNNPKVMNLISKLSAKFGGHS DIP protein P50503 original sequence version: 92 DIP protein P50503 original sequence version: 97 1AEBFC0526AE5CE1 st13-1 2 7 unspecified role unspecified role unspecified role unspecified role Region 1-44 Region 1-44 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction hsp70-st13-1 1 6 unspecified role unspecified role unspecified role unspecified role Region 1-382 Region 1-382 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 78-247 Region 78-247 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P50503:p.Asn177Lys N177K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain N K This feature has been corrected as a result of our quality control procedures. The original label was 'N177K'. Wed May 02 16:18:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn177lys' Tue Jun 30 18:18:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn177Lys' P50503:p.[Asp211Lys;Tyr212Ala] D211K/Y212A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain D K This feature has been corrected as a result of our quality control procedures. The original label was 'D211K/Y212A'. Wed May 02 19:54:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp211lys,tyr212ala' Tue Jun 30 17:58:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp211Lys;Tyr212Ala]' ecolx Escherichia coli direct interaction direct interaction st13-2 5 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction hsp70-st13-2 4 6 unspecified role unspecified role unspecified role unspecified role Region 1-382 Region 1-382 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 78-247 Region 78-247 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P50503:p.Asn177Lys N177K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain N K This feature has been corrected as a result of our quality control procedures. The original label was 'N177K'. Wed May 02 20:08:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn177lys' Tue Jun 30 19:13:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn177Lys' P50503:p.[Asn177Ala;Asp179Ala] N177A/D179A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain N A This feature has been corrected as a result of our quality control procedures. The original label was 'N177A/D179A'. Wed May 02 17:52:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn177ala,asp179ala' Tue Jun 30 17:36:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn177Ala;Asp179Ala]' P50503:p.Leu210Ser L210S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L S This feature has been corrected as a result of our quality control procedures. The original label was 'L210S'. Wed May 02 18:06:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu210ser' Tue Jun 30 18:42:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu210Ser' P50503:p.[Asp211Lys;Tyr212Ala] D211K/Y212A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain D K This feature has been corrected as a result of our quality control procedures. The original label was 'D211K/Y212A'. Wed May 02 18:43:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp211lys,tyr212ala' Tue Jun 30 19:50:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp211Lys;Tyr212Ala]' P50503:p.Lys117Ala K117A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K117A'. Wed May 02 18:48:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys117ala' Tue Jun 30 18:31:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys117Ala' P50503:p.[Arg235Ala;Lys236Asp] R235A/K236D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K D certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R235A/K236D'. Wed May 02 18:57:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg235ala,lys236asp' Tue Jun 30 19:04:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg235Ala;Lys236Asp]' P50503:p.Ser180Glu S180E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain S E This feature has been corrected as a result of our quality control procedures. The original label was 'S180E'. Wed May 02 17:34:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser180glu' Tue Jun 30 18:32:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser180Glu' P50503:p.Ile148Ala I148A mutation increasing strength mutation increasing interaction strength mutation increasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I148A'. Wed May 02 18:12:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile148ala' Tue Jun 30 16:47:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile148Ala' ecolx Escherichia coli direct interaction direct interaction Deletion of region 235-247 decreased the interaction rate/strength. hsp70-st13-3 3 6 unspecified role unspecified role unspecified role unspecified role Region 1-382 Region 1-382 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain T204A T204A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction