DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. PLoS biology 2012 imex curation Polley S., Huang DB., Hauenstein AV., Fusco AJ., Zhong X., Vu D., Schröfelbauer B., Kim Y., Hoffmann A., Verma IM., Ghosh G., Huxford T. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Polley S., Huang DB., Hauenstein AV., Fusco AJ., Zhong X., Vu D., Schröfelbauer B., Kim Y., Hoffmann A., Verma IM., Ghosh G., Huxford T. DIP original experiment: DIP-252439X PLoS biology 2012 IMEx A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. PLoS biology 2012 imex curation Polley S., Huang DB., Hauenstein AV., Fusco AJ., Zhong X., Vu D., Schröfelbauer B., Kim Y., Hoffmann A., Verma IM., Ghosh G., Huxford T. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Polley S., Huang DB., Hauenstein AV., Fusco AJ., Zhong X., Vu D., Schröfelbauer B., Kim Y., Hoffmann A., Verma IM., Ghosh G., Huxford T. DIP original experiment: DIP-252437X PLoS biology 2012 IMEx A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. PLoS biology 2012 imex curation Polley S., Huang DB., Hauenstein AV., Fusco AJ., Zhong X., Vu D., Schröfelbauer B., Kim Y., Hoffmann A., Verma IM., Ghosh G., Huxford T. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm light scattering light scattering predetermined predetermined participant predetermined Polley S., Huang DB., Hauenstein AV., Fusco AJ., Zhong X., Vu D., Schröfelbauer B., Kim Y., Hoffmann A., Verma IM., Ghosh G., Huxford T. DIP original experiment: DIP-252440X PLoS biology 2012 IMEx A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. PLoS biology 2012 imex curation Polley S., Huang DB., Hauenstein AV., Fusco AJ., Zhong X., Vu D., Schröfelbauer B., Kim Y., Hoffmann A., Verma IM., Ghosh G., Huxford T. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Polley S., Huang DB., Hauenstein AV., Fusco AJ., Zhong X., Vu D., Schröfelbauer B., Kim Y., Hoffmann A., Verma IM., Ghosh G., Huxford T. DIP original experiment: DIP-252438X PLoS biology 2012 IMEx A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. PLoS biology 2012 imex curation Polley S., Huang DB., Hauenstein AV., Fusco AJ., Zhong X., Vu D., Schröfelbauer B., Kim Y., Hoffmann A., Verma IM., Ghosh G., Huxford T. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Polley S., Huang DB., Hauenstein AV., Fusco AJ., Zhong X., Vu D., Schröfelbauer B., Kim Y., Hoffmann A., Verma IM., Ghosh G., Huxford T. DIP original experiment: DIP-252441X PLoS biology 2012 IMEx A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. ikkb_human Inhibitor of nuclear factor kappa-B kinase subunit beta IKBKB IKKB I-kappa-B kinase 2 Nuclear factor NF-kappa-B inhibitor kinase beta Serine/threonine protein kinase IKBKB protein protein human Homo sapiens Human MSWSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSDIASALRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLAERLEKWLQLMLMWHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTEDESLQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNEGHTLDMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQLKAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKLLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNASRLSQPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVREQDQSFTALDWSWLQTEEEEHSCLEQAS DIP protein O14920 original sequence version: 89 DIP protein O14920 original sequence version: 100 DIP protein O14920 original sequence version: 121 DIP protein O14920 original sequence version: 126 DIP protein O14920 original sequence version: 136 DIP protein O14920 original sequence version: 128 DIP protein O14920-1 original sequence version: 151 DIP protein O14920 original sequence version: 142 DIP protein O14920 original sequence version: 159 DIP protein O14920 original sequence version: 93 DIP protein O14920 original sequence version: 124 DIP protein O14920-1 original sequence version: 178 DIP protein O14920 original sequence version: 85 F9CADF671AE9E14E ikbkb-23 2 6 unspecified role unspecified role unspecified role unspecified role Region 11-669 Region 11-669 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O14920:p.[Ser177Glu;Ser181Glu] S177E/S181E mutation mutation certain certain sequence position certain certain certain sequence position certain S E certain certain sequence position certain certain certain sequence position certain S E This feature has been corrected as a result of our quality control procedures. The original label was 'S177E/S181E'. Wed May 02 18:01:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser177glu,ser181glu' Tue Jun 30 15:40:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser177Glu;Ser181Glu]' spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction ikbkb-ikbkb-3 5 6 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-665 Region 1-665 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O14920:p.Asp145Asn D145N mutation mutation certain certain sequence position certain certain certain sequence position certain D N This feature has been corrected as a result of our quality control procedures. The original label was 'D145N'. Wed May 02 18:50:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp145asn' Tue Jun 30 15:53:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp145Asn' pS181 pS181 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 6 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation ikbkb-6 4 6 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction ikbkb-7 1 6 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction ikbkb-8 3 6 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction