DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Nature structural & molecular biology 2013 imex curation Kassube SA., Jinek M., Fang J., Tsutakawa S., Nogales E. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kassube SA., Jinek M., Fang J., Tsutakawa S., Nogales E. DIP original experiment: DIP-254726X Nature structural & molecular biology 2013 IMEx Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Nature structural & molecular biology 2013 imex curation Kassube SA., Jinek M., Fang J., Tsutakawa S., Nogales E. Only protein-protein interactions ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Kassube SA., Jinek M., Fang J., Tsutakawa S., Nogales E. DIP original experiment: DIP-254727X Nature structural & molecular biology 2013 IMEx Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Nature structural & molecular biology 2013 imex curation Kassube SA., Jinek M., Fang J., Tsutakawa S., Nogales E. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Kassube SA., Jinek M., Fang J., Tsutakawa S., Nogales E. DIP original experiment: DIP-254728X Nature structural & molecular biology 2013 IMEx Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Nature structural & molecular biology 2013 imex curation Kassube SA., Jinek M., Fang J., Tsutakawa S., Nogales E. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Kassube SA., Jinek M., Fang J., Tsutakawa S., Nogales E. DIP original experiment: DIP-254731X Nature structural & molecular biology 2013 IMEx Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Nature structural & molecular biology 2013 imex curation Kassube SA., Jinek M., Fang J., Tsutakawa S., Nogales E. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Kassube SA., Jinek M., Fang J., Tsutakawa S., Nogales E. DIP original experiment: DIP-254730X Nature structural & molecular biology 2013 IMEx Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. Nature structural & molecular biology 2013 imex curation Kassube SA., Jinek M., Fang J., Tsutakawa S., Nogales E. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Kassube SA., Jinek M., Fang J., Tsutakawa S., Nogales E. DIP original experiment: DIP-254729X Nature structural & molecular biology 2013 IMEx Structural mimicry in transcription regulation of human RNA polymerase II by the DNA helicase RECQL5. o94762-1 ATP-dependent DNA helicase Q5 RECQL5 RECQ5 DNA helicase, RecQ-like type 5 RecQ protein-like 5 protein protein human Homo sapiens Human MSSHHTTFPFDPERRVRSTLKKVFGFDSFKTPLQESATMAVVKGNKDVFVCMPTGAGKSLCYQLPALLAKGITIVVSPLIALIQDQVDHLLTLKVRVSSLNSKLSAQERKELLADLEREKPQTKILYITPEMAASSSFQPTLNSLVSRHLLSYLVVDEAHCVSQWGHDFRPDYLRLGALRSRLGHAPCVALTATATPQVQEDVFAALHLKKPVAIFKTPCFRANLFYDVQFKELISDPYGNLKDFCLKALGQEADKGLSGCGIVYCRTREACEQLAIELSCRGVNAKAYHAGLKASERTLVQNDWMEEKVPVIVATISFGMGVDKANVRFVAHWNIAKSMAGYYQESGRAGRDGKPSWCRLYYSRNDRDQVSFLIRKEVAKLQEKRGNKASDKATIMAFDALVTFCEELGCRHAAIAKYFGDALPACAKGCDHCQNPTAVRRRLEALERSSSWSKTCIGPSQGNGFDPELYEGGRKGYGDFSRYDEGSGGSGDEGRDEAHKREWNLFYQKQMQLRKGKDPKIEEFVPPDENCPLKEASSRRIPRLTVKAREHCLRLLEEALSSNRQSTRTADEADLRAKAVELEHETFRNAKVANLYKASVLKKVADIHRASKDGQPYDMGGSAKSCSAQAEPPEPNEYDIPPASHVYSLKPKRVGAGFPKGSCPFQTATELMETTRIREQAPQPERGGEHEPPSRPCGLLDEDGSEPLPGPRGEVPGGSAHYGGPSPEKKAKSSSGGSSLAKGRASKKQQLLATAAHKDSQSIARFFCRRVESPALLASAPEAEGACPSCEGVQGPPMAPEKYTGEEDGAGGHSPAPPQTEECLRERPSTCPPRDQGTPEVQPTPAKDTWKGKRPRSQQENPESQPQKRPRPSAKPSVVAEVKGSVSASEQGTLNPTAQDPFQLSAPGVSLKEAANVVVKCLTPFYKEGKFASKELFKGFARHLSHLLTQKTSPGRSVKEEAQNLIRHFFHGRARCESEADWHGLCGPQR DIP protein O94762-1 original sequence version: 103 DIP protein O94762-1 original sequence version: 133 tcea1_human Transcription elongation factor A protein 1 TCEA1 GTF2S TFIIS Transcription elongation factor S-II protein 1 Transcription elongation factor TFIIS.o protein protein human Homo sapiens Human MEDEVVRFAKKMDKMVQKKNAAGALDLLKELKNIPMTLELLQSTRIGMSVNAIRKQSTDEEVTSLAKSLIKSWKKLLDGPSTEKDLDEKKKEPAITSQNSPEAREESTSSGNVSNRKDETNARDTYVSSFPRAPSTSDSVRLKCREMLAAALRTGDDYIAIGADEEELGSQIEEAIYQEIRNTDMKYKNRVRSRISNLKDAKNPNLRKNVLCGNIPPDLFARMTAEEMASDELKEMRKNLTKEAIREHQMAKTGGTQTDLFTCGKCKKKNCTYTQVQTRSADEPMTTFVVCNECGNRWKFC DIP protein P23193 original sequence version: 113 DIP protein P23193-1 original sequence version: 144 DIP protein P23193 original sequence version: 174 8A685107A56D2DA1 rpb1_human DNA-directed RNA polymerase II subunit RPB1 POLR2A DNA-directed RNA polymerase II subunit A DNA-directed RNA polymerase III largest subunit POLR2 RNA-directed RNA polymerase II subunit RPB1 protein protein human Homo sapiens Human MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTGMFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPGYSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPASPKYTPTSPSYSPSSPEYTPTSPKYSPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPVYTPTSPKYSPTSPTYSPTSPKYSPTSPTYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPDDSDEEN DIP protein P24928 original sequence version: 108 DIP protein P24928 original sequence version: 147 DIP protein P24928 original sequence version: 158 DIP protein P24928 original sequence version: 157 DIP protein P24928 original sequence version: 179 DIP protein P24928 original sequence version: 181 DIP protein P24928 original sequence version: 161 DIP protein P24928 original sequence version: 175 DIP protein P24928-1 original sequence version: 175 DIP protein P24928 original sequence version: 68 DIP protein P24928 original sequence version: 116 28D6FD25693A6472 recql5-1 1 7 unspecified role unspecified role unspecified role unspecified role KIX domain Region 515-620 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction polr2a-recql5-2 4 9 unspecified role unspecified role bait bait Region 1168-1302 Region 1168-1302 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 7 unspecified role unspecified role prey prey Region 490-620 Region 490-620 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction tcea1-polr2a-3 6 8 unspecified role unspecified role bait bait Region 137-301 Region 137-301 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 9 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association recql5-4 2 7 unspecified role unspecified role unspecified role unspecified role KIX domain Region 515-620 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction recql5-polr2a-4 5 9 unspecified role unspecified role prey prey Region 1168-1302 Region 1168-1302 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role bait bait Region 490-620 Region 490-620 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O94762-1:p.Trp504Ala W504A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W504A'. Wed May 02 15:50:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp504ala' Tue Jun 30 18:49:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp504Ala' O94762-1:p.Tyr508Ala Y508A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y508A'. Wed May 02 19:01:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr508ala' Tue Jun 30 18:34:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr508Ala' O94762-1:p.[Trp504Ala;Phe507Ala] W504A/F507A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W504A/F507A'. Wed May 02 17:49:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp504ala,phe507ala' Tue Jun 30 18:53:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Trp504Ala;Phe507Ala]' O94762-1:p.[Trp504Ala;Tyr508Ala] W504A/Y508A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W504A/Y508A'. Wed May 02 16:32:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp504ala,tyr508ala' Tue Jun 30 16:26:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Trp504Ala;Tyr508Ala]' O94762-1:p.[Phe507Ala;Tyr508Ala] F507A/Y508A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F507A/Y508A'. Wed May 02 17:12:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe507ala,tyr508ala' Tue Jun 30 18:43:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe507Ala;Tyr508Ala]' O94762-1:p.[Trp504Ala;Phe507Ala;Tyr508Ala] W504A/F507A/Y508A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W504A/F507A/Y508A'. Wed May 02 15:54:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp504ala,phe507ala,tyr508ala' Tue Jun 30 17:31:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Trp504Ala;Phe507Ala;Tyr508Ala]' O94762-1:p.Arg515Glu R515E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'R515E'. Wed May 02 16:39:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg515glu' Tue Jun 30 19:50:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg515Glu' O94762-1:p.Lys516Glu K516E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K516E'. Wed May 02 18:26:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys516glu' Tue Jun 30 16:28:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys516Glu' O94762-1:p.His552Ala H552A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H552A'. Wed May 02 18:57:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his552ala' Tue Jun 30 17:03:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His552Ala' O94762-1:p.Leu556Glu L556E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L E This feature has been corrected as a result of our quality control procedures. The original label was 'L556E'. Wed May 02 18:19:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu556glu' Tue Jun 30 17:30:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu556Glu' O94762-1:p.Lys598Glu K598E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K598E'. Wed May 02 15:15:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys598glu' Tue Jun 30 17:59:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys598Glu' O94762-1:p.Leu602Glu L602E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L E This feature has been corrected as a result of our quality control procedures. The original label was 'L602E'. Wed May 02 14:57:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu602glu' Tue Jun 30 15:41:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu602Glu' O94762-1:p.Lys603Glu K603E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K603E'. Wed May 02 16:58:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys603glu' Tue Jun 30 18:07:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys603Glu' O94762-1:p.[Leu556Glu;Leu602Glu] L556E/L603E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L E certain certain sequence position certain certain certain sequence position certain L E Wed May 02 15:51:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu556glu,leu602glu' Tue Jun 30 17:18:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu556Glu;Leu602Glu]' ecolx Escherichia coli direct interaction direct interaction recql5-polr2a-5 3 9 unspecified role unspecified role prey prey human Homo sapiens Human 7 unspecified role unspecified role bait bait Region 490-620 Region 490-620 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli physical association physical association