DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Small molecule inhibition of the KRAS-PDEδ interaction impairs oncogenic KRAS signalling. Small molecule inhibition of the KRAS-PDEδ interaction impairs oncogenic KRAS signalling. Nature 2013 imex curation Zimmermann G., Papke B., Ismail S., Vartak N., Chandra A., Hoffmann M., Hahn SA., Triola G., Wittinghofer A., Bastiaens PI., Waldmann H. Only protein-protein interactions in vitro In vitro alpha-screen amplified luminescent proximity homogeneous assay AlphaScreen alpha-screen AlphaScreen predetermined predetermined participant predetermined Zimmermann G., Papke B., Ismail S., Vartak N., Chandra A., Hoffmann M., Hahn SA., Triola G., Wittinghofer A., Bastiaens PI., Waldmann H. DIP original experiment: DIP-252893X Nature 2013 IMEx Small molecule inhibition of the KRAS-PDEδ interaction impairs oncogenic KRAS signalling. Small molecule inhibition of the KRAS-PDEδ interaction impairs oncogenic KRAS signalling. Small molecule inhibition of the KRAS-PDEδ interaction impairs oncogenic KRAS signalling. Nature 2013 imex curation Zimmermann G., Papke B., Ismail S., Vartak N., Chandra A., Hoffmann M., Hahn SA., Triola G., Wittinghofer A., Bastiaens PI., Waldmann H. Only protein-protein interactions canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Zimmermann G., Papke B., Ismail S., Vartak N., Chandra A., Hoffmann M., Hahn SA., Triola G., Wittinghofer A., Bastiaens PI., Waldmann H. DIP original experiment: DIP-252894X Nature 2013 IMEx Small molecule inhibition of the KRAS-PDEδ interaction impairs oncogenic KRAS signalling. pde6d_human Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta Protein p17 PDE6D PDED protein protein human Homo sapiens Human MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLVSTSRVRLFYV DIP protein O43924 original sequence version: 114 DIP protein O43924 original sequence version: 140 AB8D9309C33B4411 p01116-2 GTPase KRas KRAS KRAS2 RASK2 K-Ras 2 Ki-Ras c-K-ras c-Ki-ras K-Ras4B protein protein human Homo sapiens Human MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM DIP protein P01116-2 original sequence version: 184 DIP protein P01116-2 original sequence version: 140 DIP protein P01116-2 original sequence version: 190 DIP protein P01116-2 original sequence version: 165 Variant in position: 152:V->G (in NS3). Variant in position: 153:D->V (in CFC2 and NS3, exhibits only minor alterations in its in vitro biochemical behavior compared to wild-type protein). Variant in position: 156:F->I (in NS3/CFC2). Variant in position: 156:F->L (found in a patient with Costello syndrome, exhibits an increase in intrinsic and guanine nucleotide exchange factor catalyzed nucleotide exchange in combination with an impaired GTPase-activating protein-stimulated GTP hydrolysis but functional in interaction with effectors). B1B6D189BB259861 rheb_human GTP-binding protein Rheb Ras homolog enriched in brain RHEB RHEB2 protein protein human Homo sapiens Human MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM DIP protein Q15382 original sequence version: 146 DIP protein Q15382 original sequence version: 91 8F4C8080BDF928FD kras-pde6d-1 1 4 unspecified role unspecified role unspecified role unspecified role Region 175-185 Region 175-185 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Farnesyl Farnesyl farnres farnesylated residue FarnRes Farnesyl Farnesylation certain certain sequence position certain certain certain sequence position certain in vitro In vitro 3 unspecified role unspecified role unspecified role unspecified role His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction rheb-pde6d-1 2 5 unspecified role unspecified role fluorescence donor fluorescence donor mCitrine-tag mCitrine-tag fluorescent prot tag fluorescent protein tag fluorescent prot tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris 3 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept mCherry-tag mCherry-tag mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris proximity proximity close-contact colocalization neighbourhood interaction