DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structure (London, England : 1993) 2013 imex curation Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. Only protein-protein interactions ecolx Escherichia coli 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. DIP original experiment: DIP-252318X Structure (London, England : 1993) 2013 IMEx Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structure (London, England : 1993) 2013 imex curation Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. Only protein-protein interactions ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. DIP original experiment: DIP-252322X Structure (London, England : 1993) 2013 IMEx Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structure (London, England : 1993) 2013 imex curation Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. Only protein-protein interactions ecolx Escherichia coli atomic force microsc atomic force microscopy AFM AFM atomic force microsc predetermined predetermined participant predetermined Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. DIP original experiment: DIP-252316X Structure (London, England : 1993) 2013 IMEx Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structure (London, England : 1993) 2013 imex curation Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. DIP original experiment: DIP-252320X Structure (London, England : 1993) 2013 IMEx Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structure (London, England : 1993) 2013 imex curation Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. DIP original experiment: DIP-252317X Structure (London, England : 1993) 2013 IMEx Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structure (London, England : 1993) 2013 imex curation Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. Only protein-protein interactions ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. DIP original experiment: DIP-252319X Structure (London, England : 1993) 2013 IMEx Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structure (London, England : 1993) 2013 imex curation Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F., Feng W. DIP original experiment: DIP-252321X Structure (London, England : 1993) 2013 IMEx Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. huth_rat Histidine ammonia-lyase Hal Huth protein protein rat Rattus norvegicus (Rat) Rat MPRYTVHVRGEWLAVPCQDGKLSVGWLGREAVRRYMKNKPDNGGFTSVDEVRFLVRRCKGLGLLDNEDLLEVALEDNEFVEVVIEGDVMSPDFIPSQPEGVFLYSKYREPEKYIALDGDSLSTEDLVNLGKGHYKIKLTSIAEKKVQQSREVIDSIIKERTVVYGITTGFGKFARTVIPANKLQELQVNLVRSHSSGVGKPLSPERCRMLLALRINVLAKGYSGISLETLKQVIEVFNASCLSYVPEKGTVGASGDLAPLSHLALGLIGEGKMWSPKSGWADAKYVLEAHGLKPIVLKPKEGLALINGTQMITSLGCEAVERASAIARQADIVAALTLEVLKGTTKAFDTDIHAVRPHRGQIEVAFRFRSLLDSDHHPSEIAESHRFCDRVQDAYTLRCCPQVHGVVNDTIAFVKDIITTELNSATDNPMVFASRGETISGGNFHGEYPAKALDYLAIGVHELAAISERRIERLCNPSLSELPAFLVAEGGLNSGFMIAHCTAAALVSESKALCHPSSVDSLSTSAATEDHVSMGGWAARKALRVIEHVEQVLAIELLAACQGIEFLRPLKTTTPLEKVYDLVRSVVRPWIKDRFMAPDIEAAHRLLLDQKVWEVAAPYIEKYRMEHIPESRPLSPTAFSLESLRKNSATIPESDDL DIP protein P21213 original sequence version: 97 CBED79FC6CD40FA0 pard3_rat Partitioning defective 3 homolog Pard3 Par3 Atypical PKC isotype-specific-interacting protein Atypical PKC-specific-binding protein protein protein rat Rattus norvegicus (Rat) Rat MKVTVCFGRTRVVVPCGDGRMKVFSLIQQAVTRYRKAVAKDPNYWIQVHRLEHGDGGILDLDDILCDVADDKDRLVAVFDEQDPHHGGDGTSASSTGTQSPEIFGSELGTNNVSAFRPYQTTSEIEVTPSVLRANMPLHVRRSSDPALTGLSTSVSDNNFSSEEPSRKNPTRWSTTAGFLKQNTTGSPKTCDRKKDENYRSLPRDPSSWSNQFQRDNARSSLSASHPMVDRWLEKQEQDEEGTEEDSSRVEPVGHADTGLENMPNFSLDDMVKLVQVPNDGGPLGIHVVPFSARGGRTLGLLVKRLEKGGKAEQENLFHENDCIVRINDGDLRNRRFEQAQHMFRQAMRARVIWFHVVPAANKEQYEQLSQREMNNYSPGRFSPDSHCVANRSVANNAPQALPRAPRLSQPPEQLDAHPRLPHSAHASTKPPTAPALAPPNVLSTSVGSVYNTKRVGKRLNIQLKKGTEGLGFSITSRDVTIGGSAPIYVKNILPRGAAIQDGRLKAGDRLIEVNGVDLAGKSQEEVVSLLRSTKMEGTVSLLVFRQEEAFHPREMNAEPSQMQSPKETKAEDEDIVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKENHADLGIFVKSIINGGAASKDGRLRVNDQLIAVNGESLLGKANQEAMETLRRSMSTEGNKRGMIQLIVARRISRCNELRSPGSPAAPELPIETELDDRERRISHSLYSGIEGLDESPTRNAALSRIMGESGKCQLSPTVNMPHDDTVMIEDDRLPVLPPHLSDQSSSSSHDDVGFIMTEAGTWAKATISDSADCSLSPDVDPVLAFQREGFGRQSMSEKRTKQFSNASQLDFVKTRKSKSMDLGIADETKLNTVDDQRAGSPNRDVGPSLGLKKSSSLESLQTAVAEVTLNGNIPFHRPRPRIIRGRGCNESFRAAIDKSYDKPMVDDDDEGMETLEEDTEESSRSGRESVSTSSDQPSYSLERQMNGDPEKRDKAEKKKDKAGKDKKKDREKEKDKLKAKKGMLKGLGDMFRFGKHRKDDKMEKMGRIKIQDSFTSEEDRVRMKEEQERIQAKTREFRERQARERDYAEIQDFHRTFGCDDELLYGGMSSYDGCLALNARPQSPREGHLMDTLYAQVKKPRSSKPGDSNRSTPSNHDRIQRLRQEFQQAKQDEDVEDRRRTYSFEQSWSSSRPASQSGRHSVSVEVQVQRQRQEERESFQQAQRQYSSLPRQSRKNASSVSQDSWEQNYAPGEGFQSAKENPRYSSYQGSRNGYLGGHGFNARVMLETQELLRQEQRRKEQQLKKQPPADGVRGPFRQDVPPSPSQVARLNRLQTPEKGRPFYS DIP protein Q9Z340 original sequence version: 109 EC980C5106B52F9C hal-1 2 8 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 2-89 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction pard3-1 6 9 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 2-83 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction pard3-3 3 9 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 2-83 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction pard3-4 4 9 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 2-83 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9Z340:p.Arg9Ala R9A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R9A'. Wed May 02 19:18:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg9ala' Tue Jun 30 19:21:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg9Ala' Q9Z340:p.Thr4Glu T4E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain T E This feature has been corrected as a result of our quality control procedures. The original label was 'T4E'. Wed May 02 18:47:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr4glu' Tue Jun 30 18:29:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr4Glu' Q9Z340:p.[Val13Asp;Asp70Lys] V13D/D70K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain D K certain certain sequence position certain certain certain sequence position certain V D This feature has been corrected as a result of our quality control procedures. The original label was 'V13D/D70K'. Wed May 02 20:08:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val13asp,asp70lys' Tue Jun 30 17:21:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val13Asp;Asp70Lys]' ecolx Escherichia coli direct interaction direct interaction pard3-5 5 9 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 2-83 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction pard3-6 1 9 unspecified role unspecified role unspecified role unspecified role N-terminal domain Region 2-83 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction pard3-7 7 9 unspecified role unspecified role unspecified role unspecified role Q9Z340:p.[Val13Asp;Asp70Lys] V13D/D70K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain D K certain certain sequence position certain certain certain sequence position certain V D This feature has been corrected as a result of our quality control procedures. The original label was 'V13D/D70K'. Wed May 02 19:07:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val13asp,asp70lys' Tue Jun 30 19:52:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val13Asp;Asp70Lys]' N-terminal domain Region 2-83 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction