DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Crystal structure of pre-activated arrestin p44. Crystal structure of pre-activated arrestin p44. Nature 2013 imex curation Kim YJ., Hofmann KP., Ernst OP., Scheerer P., Choe HW., Sommer ME. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Kim YJ., Hofmann KP., Ernst OP., Scheerer P., Choe HW., Sommer ME. DIP original experiment: DIP-252862X Nature 2013 IMEx Crystal structure of pre-activated arrestin p44. Crystal structure of pre-activated arrestin p44. Crystal structure of pre-activated arrestin p44. Nature 2013 imex curation Kim YJ., Hofmann KP., Ernst OP., Scheerer P., Choe HW., Sommer ME. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Kim YJ., Hofmann KP., Ernst OP., Scheerer P., Choe HW., Sommer ME. DIP original experiment: DIP-252859X Nature 2013 IMEx Crystal structure of pre-activated arrestin p44. Crystal structure of pre-activated arrestin p44. Crystal structure of pre-activated arrestin p44. Nature 2013 imex curation Kim YJ., Hofmann KP., Ernst OP., Scheerer P., Choe HW., Sommer ME. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Kim YJ., Hofmann KP., Ernst OP., Scheerer P., Choe HW., Sommer ME. DIP original experiment: DIP-252861X Nature 2013 IMEx Crystal structure of pre-activated arrestin p44. Crystal structure of pre-activated arrestin p44. Crystal structure of pre-activated arrestin p44. Nature 2013 imex curation Kim YJ., Hofmann KP., Ernst OP., Scheerer P., Choe HW., Sommer ME. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati weight by comassie molecular weight estimation by coomasie staining weight by comassie Kim YJ., Hofmann KP., Ernst OP., Scheerer P., Choe HW., Sommer ME. DIP original experiment: DIP-252858X Nature 2013 IMEx Crystal structure of pre-activated arrestin p44. Crystal structure of pre-activated arrestin p44. Crystal structure of pre-activated arrestin p44. Nature 2013 imex curation Kim YJ., Hofmann KP., Ernst OP., Scheerer P., Choe HW., Sommer ME. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Kim YJ., Hofmann KP., Ernst OP., Scheerer P., Choe HW., Sommer ME. DIP original experiment: DIP-252860X Nature 2013 IMEx Crystal structure of pre-activated arrestin p44. opsd_bovin Rhodopsin RHO protein protein bovin Bos taurus (Bovine) Bovine MNGTEGPNFYVPFSNKTGVVRSPFEAPQYYLAEPWQFSMLAAYMFLLIMLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLVGWSRYIPEGMQCSCGIDYYTPHEETNNESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFAKTSAVYNPVIYIMMNKQFRNCMVTTLCCGKNPLGDDEASTTVSKTETSQVAPA DIP protein P02699 original sequence version: 157 DIP protein P02699 original sequence version: 158 DIP protein P02699 original sequence version: 88 DIP protein P02699 original sequence version: 160 DIP protein P02699 original sequence version: 152 DIP protein P02699 original sequence version: 136 DIP protein P02699 original sequence version: 135 DIP protein P02699 original sequence version: 141 mint Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal 33FDA196803E81F3 p08168-1 S-arrestin SAG 48 kDa protein Retinal S-antigen Rod photoreceptor arrestin protein protein bovin Bos taurus (Bovine) Bovine MKANKPAPNHVIFKKISRDKSVTIYLGKRDYIDHVERVEPVDGVVLVDPELVKGKRVYVSLTCAFRYGQEDIDVMGLSFRRDLYFSQVQVFPPVGASGATTRLQESLIKKLGANTYPFLLTFPDYLPCSVMLQPAPQDVGKSCGVDFEIKAFATHSTDVEEDKIPKKSSVRLLIRKVQHAPRDMGPQPRAEASWQFFMSDKPLRLAVSLSKEIYYHGEPIPVTVAVTNSTEKTVKKIKVLVEQVTNVVLYSSDYYIKTVAAEEAQEKVPPNSSLTKTLTLVPLLANNRERRGIALDGKIKHEDTNLASSTIIKEGIDKTVMGILVSYQIKVKLTVSGLLGELTSSEVATEVPFRLMHPQPEDPDTAKESFQDENFVFEEFARQNLKDAGEYKEEKTDQEAAMDE DIP protein P08168-1 original sequence version: 130 DIP protein P08168 original sequence version: 125 DIP protein P08168-1 original sequence version: 81 DIP protein P08168-1 original sequence version: 114 rho-sag-19 4 6 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction rho-sag-20 3 6 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 7 unspecified role unspecified role unspecified role unspecified role Bimane-label Bimane-label bimane bimane label bimane certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-21 2 6 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 7 unspecified role unspecified role unspecified role unspecified role Bimane-label Bimane-label bimane bimane label bimane certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-22 1 6 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 7 unspecified role unspecified role unspecified role unspecified role Bimane-label Bimane-label bimane bimane label bimane certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-23 5 6 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 7 unspecified role unspecified role unspecified role unspecified role Bimane-label Bimane-label bimane bimane label bimane certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction