IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions human-jurkat Homo sapiens jurkat Human T cell leukemia pull down pull down western blot western blot Immuno blot Immuno blot TIme after addition of anti-CD3 mAb second second sec s s sec 150 0 120 Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. awaiting a developer to remove annotation in expt-10- July 6th email sent Details of the Human GRB2 mutant plasmids gifted by Dr. R.A. Weinberg were obtained from Addgene. It has been assumed the WT construct is similar. oreste.acuto@path.ox.ac.uk Accepted 2017-AUG-03 AT 11:32 BST AT 11:32 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation altered; altered; I have moved the mutant interaction to Expt-12; variable annotation removed by Anjali. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro peptide array peptide array tag visualisation tag visualisation Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. awaiting a developer to remove annotation in expt-10- July 6th email sent oreste.acuto@path.ox.ac.uk Accepted 2017-AUG-03 AT 11:32 BST AT 11:32 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation altered; altered; I have moved the mutant interaction to Expt-12; variable annotation removed by Anjali. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions human-jurkat Homo sapiens jurkat Human T cell leukemia pull down pull down western blot western blot Immuno blot Immuno blot Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. awaiting a developer to remove annotation in expt-10- July 6th email sent oreste.acuto@path.ox.ac.uk Accepted 2017-AUG-03 AT 11:32 BST AT 11:32 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation altered; altered; I have moved the mutant interaction to Expt-12; variable annotation removed by Anjali. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions human-jurkat Homo sapiens jurkat Human T cell leukemia pull down pull down western blot western blot Immuno blot Immuno blot Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. awaiting a developer to remove annotation in expt-10- July 6th email sent LAT-deficient Jurkat J.CaM2.5 cells were used. LAT was determined to be of Human origin and with identifier O43561-2 by mutation mapping. oreste.acuto@path.ox.ac.uk Accepted 2017-AUG-03 AT 11:32 BST AT 11:32 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation altered; altered; I have moved the mutant interaction to Expt-12; variable annotation removed by Anjali. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. awaiting a developer to remove annotation in expt-10- July 6th email sent oreste.acuto@path.ox.ac.uk Accepted 2017-AUG-03 AT 11:32 BST AT 11:32 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation altered; altered; I have moved the mutant interaction to Expt-12; variable annotation removed by Anjali. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. awaiting a developer to remove annotation in expt-10- July 6th email sent oreste.acuto@path.ox.ac.uk Accepted 2017-AUG-03 AT 11:32 BST AT 11:32 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation altered; altered; I have moved the mutant interaction to Expt-12; variable annotation removed by Anjali. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions human-jurkat Homo sapiens jurkat Human T cell leukemia pull down pull down western blot western blot Immuno blot Immuno blot TIme after addition of anti-CD3 mAb second second sec s s sec 0 120 Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. awaiting a developer to remove annotation in expt-10- July 6th email sent oreste.acuto@path.ox.ac.uk Accepted 2017-AUG-03 AT 11:32 BST AT 11:32 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation altered; altered; I have moved the mutant interaction to Expt-12; variable annotation removed by Anjali. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down western blot western blot Immuno blot Immuno blot Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. awaiting a developer to remove annotation in expt-10- July 6th email sent oreste.acuto@path.ox.ac.uk Accepted 2017-AUG-03 AT 11:32 BST AT 11:32 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation altered; altered; I have moved the mutant interaction to Expt-12; variable annotation removed by Anjali. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions human-jurkat Homo sapiens jurkat Human T cell leukemia pull down pull down western blot western blot Immuno blot Immuno blot Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. awaiting a developer to remove annotation in expt-10- July 6th email sent oreste.acuto@path.ox.ac.uk Accepted 2017-AUG-03 AT 11:32 BST AT 11:32 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation altered; altered; I have moved the mutant interaction to Expt-12; variable annotation removed by Anjali. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay sequence tag sequence tag identification sequence tag Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. awaiting a developer to remove annotation in expt-10- July 6th email sent oreste.acuto@path.ox.ac.uk Accepted 2017-AUG-03 AT 11:32 BST AT 11:32 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation altered; altered; I have moved the mutant interaction to Expt-12; variable annotation removed by Anjali. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. oreste.acuto@path.ox.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down anti tag western anti tag western blot anti tag western Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O., Paster W., Brockmeyer C., Fu G., Simister PC., de Wet B., Martinez-Riaño A., Hoerter JA., Feller SM., Wülfing C., Gascoigne NR., Acuto O. awaiting a developer to remove annotation in expt-10- July 6th email sent Details of the Human GRB2 mutant plasmids gifted by Dr. R.A. Weinberg were obtained from Addgene. It has been assumed the WT construct is similar. oreste.acuto@path.ox.ac.uk Accepted 2017-AUG-03 AT 11:32 BST AT 11:32 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2013 imex curation altered; altered; I have moved the mutant interaction to Expt-12; variable annotation removed by Anjali. lat_human Linker for activation of T-cells family member 1 LAT 36 kDa phospho-tyrosine adapter protein p36-38 protein protein human Homo sapiens Human MEEAILVPCVLGLLLLPILAMLMALCVHCHRLPGSYDSTSSDSLYPRGIQFKRPHTVAPWPPAYPPVTSYPPLSQPDLLPIPRSPQPLGGSHRTPSSRRDSDGANSVASYENEGASGIRGAQAGWGVWGPSWTRLTPVSLPPEPACEDADEDEDDYHNPGYLVVLPDSTPATSTAAPSAPALSTPGIRDSAFSMESIDDYVNVPESGESAEASLDGSREYVNVSQELHPGAAKTEPAALSSQEAEEVEEEGAPDYENLQELN BCD80AE7DCA64153 grb2_human Growth factor receptor-bound protein 2 GRB2 ASH Adapter protein GRB2 SH2/SH3 adapter GRB2 Protein Ash protein protein human Homo sapiens Human MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV 83A4B0BA1B248DC4 thms1_mouse Protein THEMIS Themis Grb2-associating protein Protein thylex Thymocyte-expressed molecule involved in selection protein protein mouse Mus musculus Mouse MALSLEEFVYSLDLRTLPRVLEIQSGIYFEGSVYEMFGNECCLSTGEVIKITGLKIKKMMAEICEGAIGGCESQKPFELPMNFPGLFKVMADKTPYLSIEEITRTVNIGPSRLGHPCFYHLKDIKLENLIIKQGEPIRFNSVEEINGETLVNCGVVRNHQSHSFTLPLSQEGEFYECEDEHIYTLKEIVEWKIPKNRTRTVKLTDFSNKWDSTNPFPEDFYGTLILKPVYEIQGVLKFQKDIVRILPSLDVEVKDITDSYDANWFLQLLSTDDLFEMTSKEFPVVAEVVEISQGNHLPQSILQREKTIVIHKKYQASRILASEIRSNFPKRHFLIPISYKGKFKRRPREFPTAYDLQIAKSRKETLHVVATKAFHTLHKELSPVSVGDQFLVHHSETTEVVFEGTRKVNVLTCEKVLNKTREDAQLPLYMEGGFVEVIHDKKQYQISELCTQFCWPFNVKVAVRDLSIKDDILAATPGLQLEEDITDSYLLISDFANPEECWEIPMSRLNMTVRLVNGSSLPADAGLLQVRSFVEEITEEQYYMMRRYESSLSHPPPRPPKHPSAEEMKLTLLSLAEERTINLPKSLKSHHVDRPKKLPSDESGQDSRAPVGFQNDVADVERQKSKHGPLQPQAPL 695B904A539110B8 plcg1_human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 PLC-148 PLC1 Phospholipase C-II PLCG1 Phosphoinositide phospholipase C-gamma-1 Phospholipase C-gamma-1 protein protein human Homo sapiens Human MAGAASPCANGCGPGAPSDAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIEGAIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWLMEDTLQAPTPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDLEQRSGDITYGQFAQLYRSLMYSAQKTMDLPFLEASTLRAGERPELCRVSLPEFQQFLLDYQGELWAVDRLQVQEFMLSFLRDPLREIEEPYFFLDEFVTFLFSKENSVWNSQLDAVCPDTMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQYFKKVLGDTLLTKPVEISADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSMMYSENDISNSIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEVSSSTELHSNEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFIKSAIIQNVEKQEGGWWRGDYGGKKQLWFPSNYVEEMVNPVALEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMASVAHWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMNQALFMTGRHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCAISIEVLGARHLPKNGRGIVCPFVEIEVAGAEYDSTKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDIFPAKENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL DIP protein P19174 original sequence version: 144 DIP protein P19174 original sequence version: 152 DIP protein P19174 original sequence version: 181 DIP protein P19174 original sequence version: 169 AE05ABE2A18EDDAC thms1_human Protein THEMIS THEMIS C6orf190 C6orf207 Thymocyte-expressed molecule involved in selection protein protein human Homo sapiens Human MALSLEEFVHSLDLRTLPRVLEIQAGIYLEGSIYEMFGNECCFSTGEVIKITGLKVKKIIAEICEQIEGCESLQPFELPMNFPGLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK 30B5325242212E94 lck_human Tyrosine-protein kinase Lck Protein YT16 Proto-oncogene Lck T cell-specific protein-tyrosine kinase LCK Lymphocyte cell-specific protein-tyrosine kinase p56-LCK Leukocyte C-terminal Src kinase protein protein human Homo sapiens Human MGCGCSSHPEDDWMENIDVCENCHYPIVPLDGKGTLLIRNGSEVRDPLVTYEGSNPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHYTNASDGLCTRLSRPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFTATEGQYQPQP DIP protein P06239 original sequence version: 164 DIP protein P06239-1 original sequence version: 193 44BFF0D43FFB420D o43561-2 Linker for activation of T-cells family member 1 36 kDa phospho-tyrosine adapter protein p36-38 LAT Short protein protein human Homo sapiens Human MEEAILVPCVLGLLLLPILAMLMALCVHCHRLPGSYDSTSSDSLYPRGIQFKRPHTVAPWPPAYPPVTSYPPLSQPDLLPIPRSPQPLGGSHRTPSSRRDSDGANSVASYENEEPACEDADEDEDDYHNPGYLVVLPDSTPATSTAAPSAPALSTPGIRDSAFSMESIDDYVNVPESGESAEASLDGSREYVNVSQELHPGAAKTEPAALSSQEAEEVEEEGAPDYENLQELN DIP protein O43561-2 original sequence version: 110 DIP protein O43561-2 original sequence version: 58 mint 0832E2D2B4220BC6 q8n1k5-1 Protein THEMIS THEMIS C6orf190 C6orf207 Thymocyte-expressed molecule involved in selection protein protein human Homo sapiens Human MALSLEEFVHSLDLRTLPRVLEIQAGIYLEGSIYEMFGNECCFSTGEVIKITGLKVKKIIAEICEQIEGCESLQPFELPMNFPGLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKEFPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFPTAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKSYEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYLLISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKHPSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAIAETFKNEKHQK 30B5325242212E94 zap70_human Tyrosine-protein kinase ZAP-70 ZAP70 SRK Syk-related tyrosine kinase 70 kDa zeta-chain associated protein protein protein human Homo sapiens Human MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPCNRPSGLEPQPGVFDCLRDAMVRDYVRQTWKLEGEALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGAQTDGKFLLRPRKEQGTYALSLIYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSASNASGAAAPTLPAHPSTLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA DIP protein P43403 original sequence version: 146 DIP protein P43403-1 original sequence version: 167 D1E1A8EC66FA116F themis-grb2-3 5 18 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SH3_C_terminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) 24 unspecified role unspecified role bait bait partial_PRR1_sequence required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q8N1K5-1:p.Pro554Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain P A Wed May 02 15:36:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro554ala' Tue Jun 30 17:21:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro554Ala' Q8N1K5-1:p.Pro557Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain P A Wed May 02 15:25:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro557ala' Tue Jun 30 17:25:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro557Ala' Q8N1K5-1:p.Lys559Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A Fri Jun 30 13:56:06 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'lys560ala' Wed May 02 17:45:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys559ala' Tue Jun 30 18:53:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys559Ala' Q8N1K5-1:p.Arg556Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:37:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg556ala' Tue Jun 30 19:52:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg556Ala' PRR1_sequence sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 36 29 direct interaction direct interaction Figure 1 A-C the "suff. to bind" sequence is shown at the bottom of fig 1C. Binding of full-length GRB2 and GRB2-SH3C was observed only to 29-mers containing the intact PRR1 sequence. Binding of full-length GRB2 and GRB2-SH3C was observed only to 29-mers containing the intact PRR1 sequence. themis-grb2-9 16 18 unspecified role unspecified role prey prey c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P62993:p.[Pro49Leu;Gly203Arg] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain P L certain certain sequence position certain certain certain sequence position certain G R Wed May 02 16:29:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro49leu,gly203arg' Tue Jun 30 20:25:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro49Leu;Gly203Arg]' P62993:p.Pro49Leu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain P L Wed May 02 18:35:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro49leu' Tue Jun 30 18:12:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro49Leu' P62993:p.Gly203Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G R Wed May 02 20:13:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly203arg' Tue Jun 30 16:58:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly203Arg' 24 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 1D lat-grb2-4 7 23 unspecified role unspecified role bait bait range strep tag strep tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O43561-2:p.[Tyr171Phe;Tyr191Phe;Tyr226Phe] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F Fri Jun 30 13:56:31 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr171phe, tyr191phe, tyr226phe' Wed May 02 17:31:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr171phe,tyr191phe,tyr226phe' Tue Jun 30 18:45:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr171Phe;Tyr191Phe;Tyr226Phe]' 21 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey association association 2C anti-Cd3 mAb Sodium pervanadate (CHEBI:35607) the TCR-induced LAT association to GRB2 and THEMIS was lost Using the LAT 3F mutant as Bait, the interaction is reduced by a greater amount in anti-CD3 stimulated cells as compared to Pervanadate stimulated cells. 2342-1 8 18 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q8BGW0:p.[Pro557Ala;Pro560Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain P A Wed May 02 17:46:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro557ala,pro560ala' Tue Jun 30 18:58:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro557Ala;Pro560Ala]' physical association physical association 3A themis-grb2-8 13 25 unspecified role unspecified role prey prey tyr493 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 22 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association 4E themis-lck-1 13 25 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 4Ei There was a strong ZAP70-pY493 signal only when Lck was coexpressed. a strong ZAP70-pY493 signal only when Lck was coexpressed themis-grb2-10 10 20 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 18 unspecified role unspecified role prey prey association association 11 2B themis-grb2-11 1 24 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q8N1K5-1:p.Tyr540_Tyr541delinsPhePhe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain YY FF Wed May 02 16:53:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr_tyr540-541phe_phe' Tue Jun 30 20:00:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr540_Tyr541delinsPhePhe' 18 unspecified role unspecified role prey prey physical association physical association 5B themis-grb2-12 1 17 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q8N1K5-1:p.Tyr540_Tyr541delinsPhePhe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain YY FF Wed May 02 15:08:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr_tyr540-541phe_phe' Tue Jun 30 15:38:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr540_Tyr541delinsPhePhe' 18 unspecified role unspecified role prey prey association association 4 5Bi themis-grb2-5 10 17 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association 12 2B themis-grb2-4 5 18 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SH3_C_terminal_domain sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) 24 unspecified role unspecified role bait bait region_including_PRR1_sequence sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tyr540 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain Y pY observed-ptm observed-ptm observed ptm observed-ptm tyr541 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm Q8N1K5-1:p.Tyr540_Tyr541delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain YY AA Fri Jun 30 14:24:00 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr540ala,tyr541ala' Wed May 02 15:22:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr_tyr540-541ala_ala' Tue Jun 30 16:48:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr540_Tyr541delinsAlaAla' Q8N1K5-1:p.Tyr540_Tyr541delinsPhePhe mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain YY FF Fri Jun 30 13:55:04 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr540phe,tyr541phe' Wed May 02 19:55:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr_tyr540-541phe_phe' Tue Jun 30 20:02:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr540_Tyr541delinsPhePhe' chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Figure 5C themis-zap70-1 13 18 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range range optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8N1K5-1:p.Tyr540_Tyr541delinsPhePhe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain YY FF Fri Jun 30 14:26:32 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr540phe,tyr541phe' Wed May 02 19:03:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr_tyr540-541phe_phe' Tue Jun 30 17:52:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr540_Tyr541delinsPhePhe' whole_molecule required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q8N1K5-1:p.[Pro555Ala;Pro558Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain P A Wed May 02 17:43:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro555ala,pro558ala' Tue Jun 30 17:03:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro555Ala;Pro558Ala]' physical association physical association 5D, 5F No obvious changes in GRB2 associated to an increasingly phosphorylated THEMIS were detected. It is stated that :-"a soluble fragment of THEMIS lacking the CABIT-1 domain (aas 1-260) did not bind GRB2". However the isolated CABIT-2 domain (aas 261-641) did not bind GRB2 either. lck-themis-1 15 24 enzyme target enzyme target substrate substrate neutral component neutral component c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range tyr95,tyr174,tyr220,tyr353,tyr429,tyr540, tyr541 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 22 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. phosphorylation phosphorylation reaction phosphorylation Supp Table 1A P06239 (GO:0004672) up-regulates activity Q8N1K5-1 (GO:0005515) lck-themis-3 9 24 enzyme target enzyme target substrate substrate neutral component neutral component c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range phosphotyrosine_residues optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined resulting-ptm resulting-ptm resulting ptm resulting-ptm human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 22 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. phosphorylation phosphorylation reaction phosphorylation Fig 5E lck-themis-2 9 18 stimulator stimulator neutral component neutral component range gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 24 enzyme target enzyme target substrate substrate neutral component neutral component c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range phosphotyrosine_residues optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined resulting-ptm resulting-ptm resulting ptm resulting-ptm human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 22 enzyme enzyme neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. phosphorylation phosphorylation reaction phosphorylation Fig 5Ei Moreover, recombinant THEMIS purified as a monomer without GRB2 was poorly phosphorylated by Lck in vitro, whereas addition of recombinant GRB2 reconstituted THEMIS tyrosine phosphorylation Addition of recombinant GRB2 reconstituted THEMIS tyrosine phosphorylation. themis-grb2-6 14 24 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q8N1K5-1:p.[Pro555Ala;Pro558Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain P A Wed May 02 17:28:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro555ala,pro558ala' Tue Jun 30 18:27:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro555Ala;Pro558Ala]' Q8N1K5-1:p.[Pro582Ala;Pro585Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain P A Wed May 02 20:12:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro582ala,pro585ala' Tue Jun 30 19:27:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro582Ala;Pro585Ala]' 18 unspecified role unspecified role prey prey physical association physical association 2A themis-zap70-2 15 25 enzyme enzyme neutral component neutral component 24 enzyme target enzyme target substrate substrate neutral component neutral component c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range tyr95,tyr174,tyr220,tyr353,tyr429,tyr540 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation Supp Table 1B P43403 (GO:0004672) up-regulates activity Q8N1K5-1 (GO:0005515) themis-plcg1-2 6 20 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait c_terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q8N1K5-1:p.[Pro555Ala;Pro558Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain P A Wed May 02 19:21:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro555ala,pro558ala' Tue Jun 30 17:02:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro555Ala;Pro558Ala]' physical association physical association 2Bii