DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. Nature 2013 imex curation Atwood SX., Li M., Lee A., Tang JY., Oro AE. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Atwood SX., Li M., Lee A., Tang JY., Oro AE. DIP original experiment: DIP-252104X Nature 2013 IMEx GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. Nature 2013 imex curation Atwood SX., Li M., Lee A., Tang JY., Oro AE. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Atwood SX., Li M., Lee A., Tang JY., Oro AE. DIP original experiment: DIP-252107X Nature 2013 IMEx GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. Nature 2013 imex curation Atwood SX., Li M., Lee A., Tang JY., Oro AE. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Atwood SX., Li M., Lee A., Tang JY., Oro AE. DIP original experiment: DIP-252105X Nature 2013 IMEx GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. Nature 2013 imex curation Atwood SX., Li M., Lee A., Tang JY., Oro AE. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Atwood SX., Li M., Lee A., Tang JY., Oro AE. DIP original experiment: DIP-252102X Nature 2013 IMEx GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. Nature 2013 imex curation Atwood SX., Li M., Lee A., Tang JY., Oro AE. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Atwood SX., Li M., Lee A., Tang JY., Oro AE. DIP original experiment: DIP-252103X Nature 2013 IMEx GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. Nature 2013 imex curation Atwood SX., Li M., Lee A., Tang JY., Oro AE. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Atwood SX., Li M., Lee A., Tang JY., Oro AE. DIP original experiment: DIP-252100X Nature 2013 IMEx GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. Nature 2013 imex curation Atwood SX., Li M., Lee A., Tang JY., Oro AE. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Atwood SX., Li M., Lee A., Tang JY., Oro AE. DIP original experiment: DIP-252101X Nature 2013 IMEx GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. Nature 2013 imex curation Atwood SX., Li M., Lee A., Tang JY., Oro AE. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Atwood SX., Li M., Lee A., Tang JY., Oro AE. DIP original experiment: DIP-252106X Nature 2013 IMEx GLI activation by atypical protein kinase C ι/λ regulates the growth of basal cell carcinomas. kpci_human Protein kinase C iota type nPKC-iota Atypical protein kinase C-lambda/iota PRKCI DXS1179E protein protein human Homo sapiens Human MPTQRDSSTMSHTVAGGGSGDHSHQVRVKAYYRGDIMITHFEPSISFEGLCNEVRDMCSFDNEQLFTMKWIDEEGDPCTVSSQLELEEAFRLYELNKDSELLIHVFPCVPERPGMPCPGEDKSIYRRGARRWRKLYCANGHTFQAKRFNRRAHCAICTDRIWGLGRQGYKCINCKLLVHKKCHKLVTIECGRHSLPQEPVMPMDQSSMHSDHAQTVIPYNPSSHESLDQVGEEKEAMNTRESGKASSSLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSLSVKAASVLKSFLNKDPKERLGCHPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNEPVQLTPDDDDIVRKIDQSEFEGFEYINPLLMSAEECV DIP protein P41743 original sequence version: 146 1E3F8C1D4BFC734F q99nh2-1 Partitioning defective 3 homolog Pard3 Par3 180 kDa Atypical PKC isotype-specific-interacting protein Ephrin-interacting protein protein protein mouse Mus musculus Mouse MKVTVCFGRTRVVVPCGDGRMKVFSLIQQAVTRYRKAVAKDPNYWIQVHRLEHGDGGILDLDDILCDVADDKDRLVAVFDEQDPHHGGDGTSASFTGTQSPEIFGSELGTNNVSAFQPYQATSEIEVTPSVLRANMPLHVRRSSDPALTGLSTSVSDNNFSSEEPSRKNPTRWSTTAGFLKQNTAGSPKTCDRKKDENYRSLPRDPSSWSNQFQRDNARSSLSASHPMVDRWLEKQEQDEEGTEEDSSRVEPVGHADTGLENMPNFSLDDMVKLVQVPNDGGPLGIHVVPFSARGGRTLGLLVKRLEKGGKAEQENLFHENDCIVRINDGDLRNRRFEQAQHMFRQAMRARVIWFHVVPAANKEQYEQLSQREKNNYSPGRFSPDSHCVANRSVANNAPQALPRAPRLSQPPEQLDAHPRLPHSAHASTKPPAAPALAPPSVLSTNVGSVYNTKKVGKRLNIQLKKGTEGLGFSITSRDVTIGGSAPIYVKNILPRGAAIQDGRLKAGDRLIEVNGVDLAGKSQEEVVSLLRSTKMEGTVSLLVFRQEEAFHPREMNAEPSQMQTPKETKAEDEDVVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKENHADLGIFVKSIINGGAASKDGRLRVNDQLIAVNGESLLGKANQEAMETLRRSMSTEGNKRGMIQLIVARRISRCNELRSPGSPAAPELPIETELDDRERRISHSLYSGIEGLDESPTRNAALSRIMGKCQLSPTVNMPHDDTVMIEDDRLPVLPPHLSDQSSSSSHDDVGFIMTEAGTWAKATISDSADCSLSPDVDPVLAFQREGFGRQSMSEKRTKQFSDASQLDFVKTRKSKSMDLVADETKLNTVDDQRAGSPSRDVGPSLGLKKSSSLESLQTAVAEVTLNGNIPFHRPRPRIIRGRGCNESFRAAIDKSYDKPMVDDDDEGMETLEEDTEESSRSGRESVSTSSDQPSYSLERQMNGDPEKRDKTERKKDKAGKDKKKDREKEKDKLKAKKGMLKGLGDMFRFGKHRKDDKMEKMGRIKIQDSFTSEEDRVRMKEEQERIQAKTREFRERQARERDYAEIQDFHRTFGCDDELLYGGMSSYEGCLALNARPQSPREGHLMDTLYAQVKKPRSSKPGDSNRSTPSNHDRIQRLRQEFQQAKQDEDVEDRRRTYSFEQSWSSSRPASQSGRHSVSVEVQVQRQRQEERESFQQAQRQYSSLPRQSRKNASSISQDSWEQNYAPGEGFQSAKENPRYSSYQGSRNGYLGGHGFNARVMLETQELLRQEQRRKEQQLKKQPPADGVRGPFRQDVPPSPSQVARLNRLQTPEKGRPFYS DIP protein Q99NH2-1 original sequence version: 101 DIP protein Q99NH2 original sequence version: 115 DIP protein Q99NH2 original sequence version: 104 kpci_mouse Protein kinase C iota type Prkci nPKC-iota Atypical protein kinase C-lambda/iota Pkcl protein protein mouse Mus musculus Mouse MPTQRDSSTMSHTVACGGGGDHSHQVRVKAYYRGDIMITHFEPSISFEGLCSEVRDMCSFDNEQPFTMKWIDEEGDPCTVSSQLELEEAFRLYELNKDSELLIHVFPCVPERPGMPCPGEDKSIYRRGARRWRKLYCANGHTFQAKRFNRRAHCAICTDRIWGLGRQGYKCINCKLLVHKKCHKLVTIECGRHSLPPEPMMPMDQTMHPDHTQTVIPYNPSSHESLDQVGEEKEAMNTRESGKASSSLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSLSVKAASVLKSFLNKDPKERLGCHPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNEPVQLTPDDDDIVRKIDQSEFEGFEYINPLLMSAEECV DIP protein Q62074 original sequence version: 139 DIP protein Q62074 original sequence version: 131 6AC612D1E9264825 mtss1_mouse Protein MTSS 1 Mtss1 Mim Missing in metastasis protein Metastasis suppressor protein 1 protein protein mouse Mus musculus Mouse MEAVIEKECSALGGLFQTIISDMKGSYPVWEDFINKAGKLQSQLRTTVVAAAAFLDAFQKVADMATNTRGGTREIGSALTRMCMRHRSIEAKLRQFSSALIDCLINPLQEQMEEWKKVANQLDKDHAKEYKKARQEIKNKSSDTLKLQKKAKKVDAQGRGDIQPQLDSALQDVNDKYLLLEETEKQAVRKALIEERGRFCTFISMLRPVIEEEISMLGEITHLQTISEDLKSLTMDPHKLPSSSEQVILDLKGSDYSWSYQTPPSSPSTTMSRKSSVCSSLNSVNSSDSRSSGSHSHSPSSHYRYRSSNLAQQAPVRLSSVSSHDSGFISQDAFQSKSPSPMPPEAANQLSNGFSHCSLSSESHAGPVGAGPFPHCLPASRLLPRVTSVHLPDYAHYYTIGPGMFPSSQIPSWKDWAKPGPYDQPLVNTLQRRKEKREPDSNGGGPTTTGGPPAGAEEAQRPRSMTVSAATRPGEEMAACEELTLALSRGLQLDVQRSSRDSLQCSSGYSTQTTTPCCSEDTIPSQVSDYDYFSVSGDQEAEQQEFDKSSTIPRNSDISQSYRRMFQAKRPASTAGLPTTLGPAMVTPGVATIRRTPSTKPSVRRGTIGAGPIPIKTPVIPVKTPTVPDLPGVLPSPPDGPEERGEHSPESPSAGEGPQGVSNIPSSLWSGQAPVNPPLPGPKPSIPEEHRQAIPESEAEDQERDPPSATVSPGPIPESDPADLSPRESPQGEDMLNAIRRGVKLKKTTTNDRSAPRFS DIP protein Q8R1S4 original sequence version: 36 DIP protein Q8R1S4 original sequence version: 85 par6a_mouse Partitioning defective 6 homolog alpha Pard6a Par6a protein protein mouse Mus musculus Mouse MARPQRTPARSPDSIVEVKSKFDAEFRRFALPRTSVRGFQEFSRLLCVVHQIPGLDVLLGYTDAHGDLLPLTNDDSLHRALASGPPPLRLLVQKRAEGDSSGLAFASNSLQRRKKGLLLRPVAPLRTRPPLLISLPQDFRQVSSVIDVDLLPETHRRVRLHKHGSDRPLGFYIRDGMSVRVAPQGLERVPGIFISRLVRGGLAESTGLLAVSDEILEVNGIEVAGKTLDQVTDMMVANSHNLIVTVKPANQRNNVVRGASGRLTGPSSVGPGPTDPDSDDDSSDLVIENRHPPCSNGLSQGPLCWDLQPGCLLPGAGSSLPSLDSREQANSGWGNGMRGDVSGFSL DIP protein Q9Z101 original sequence version: 97 8F150B83B7E1F36A p08151-1 Zinc finger protein GLI1 GLI1 GLI Glioma-associated oncogene Oncogene GLI protein protein human Homo sapiens Human MFNSMTPPPISSYGEPCCLRPLPSQGAPSVGTEGLSGPPFCHQANLMSGPHSYGPARETNSCTEGPLFSSPRSAVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPAQMNHQKGPSPSFGVQPCGPHDSARGGMIPHPQSRGPFPTCQLKSELDMLVGKCREEPLEGDMSSPNSTGIQDPLLGMLDGREDLEREEKREPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEHEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAPSISTVEPKREREGGPIREESRLTVPEGAMKPQPSPGAQSSCSSDHSPAGSAANTDSGVEMTGNAGGSTEDLSSLDEGPCIAGTGLSTLRRLENLRLDQLHQLRPIGTRGLKLPSLSHTGTTVSRRVGPPVSLERRSSSSSSISSAYTVSRRSSLASPFPPGSPPENGASSLPGLMPAQHYLLRARYASARGGGTSPTAASSLDRIGGLPMPPWRSRAEYPGYNPNAGVTRRASDPAQAADRPAPARVQRFKSLGCVHTPPTVAGGGQNFDPYLPTSVYSPQPPSITENAAMDARGLQEEPEVGTSMVGSGLNPYMDFPPTDTLGYGGPEGAAAEPYGARGPGSLPLGPGPPTNYGPNPCPQQASYPDPTQETWGEFPSHSGLYPGPKALGGTYSQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGPPHPQPLFSHYPQPSPPQYLQSGPYTQPPPDYLPSEPRPCLDFDSPTHSTGQLKAQLVCNYVQSQQELLWEGGGREDAPAQEPSYQSPKFLGGSQVSPSRAKAPVNTYGPGFGPNLPNHKSGSYPTPSPCHENFVVGANRASHRAAAPPRLLPPLPTCYGPLKVGGTNPSCGHPEVGRLGGGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEPQGLSPPPSHDQRGSSGHTPPPSGPPNMAVGNMSVLLRSLPGETEFLNSSA DIP protein P08151-1 original sequence version: 149 prkci-mtss1-1 4 11 unspecified role unspecified role bait bait mouse Mus musculus Mouse 12 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association gli1-prkci-1 3 9 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 14 unspecified role unspecified role bait bait Region 227-398 Region 227-398 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction mtss1-pard3-1 6 13 unspecified role unspecified role prey prey mouse Mus musculus Mouse 10 unspecified role unspecified role prey prey mouse Mus musculus Mouse 11 unspecified role unspecified role prey prey mouse Mus musculus Mouse 12 unspecified role unspecified role bait bait mouse Mus musculus Mouse association association pard3-mtss1-1 5 10 unspecified role unspecified role bait bait mouse Mus musculus Mouse 12 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association gli1-prkci-2 2 9 enzyme enzyme unspecified role unspecified role His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 14 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 227-398 Region 227-398 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P08151-1:p.[Ser243Ala;Thr304Ala] S243A/T304A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S243A/T304A'. Wed May 02 16:57:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser243ala,thr304ala' Tue Jun 30 18:01:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser243Ala;Thr304Ala]' ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation mtss1-prkci-1 7 11 unspecified role unspecified role prey prey mouse Mus musculus Mouse 12 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association prkci-mtss1-2 1 11 unspecified role unspecified role bait bait mouse Mus musculus Mouse 12 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association prkci-gli1-1 8 9 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 14 unspecified role unspecified role prey prey Region 227-398 Region 227-398 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction