DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome. Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome. Nature structural & molecular biology 2013 imex curation Patrick AN., Cabrera JH., Smith AL., Chen XS., Ford HL., Zhao R. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Patrick AN., Cabrera JH., Smith AL., Chen XS., Ford HL., Zhao R. DIP original experiment: DIP-252979X Nature structural & molecular biology 2013 IMEx Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome. Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome. Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome. Nature structural & molecular biology 2013 imex curation Patrick AN., Cabrera JH., Smith AL., Chen XS., Ford HL., Zhao R. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Patrick AN., Cabrera JH., Smith AL., Chen XS., Ford HL., Zhao R. DIP original experiment: DIP-252978X Nature structural & molecular biology 2013 IMEx Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome. Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome. Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome. Nature structural & molecular biology 2013 imex curation Patrick AN., Cabrera JH., Smith AL., Chen XS., Ford HL., Zhao R. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Patrick AN., Cabrera JH., Smith AL., Chen XS., Ford HL., Zhao R. DIP original experiment: DIP-252977X Nature structural & molecular biology 2013 IMEx Structure-function analyses of the human SIX1-EYA2 complex reveal insights into metastasis and BOR syndrome. six1_human Homeobox protein SIX1 Sine oculis homeobox homolog 1 SIX1 protein protein human Homo sapiens Human MSMLPSFGFTQEQVACVCEVLQQGGNLERLGRFLWSLPACDHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHPKLQQLWLKAHYVEAEKLRGRPLGAVGKYRVRRKFPLPRTIWDGEETSYCFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDSLLGPLTSSLVDLGS DIP protein Q15475 original sequence version: 116 A4195376CFB9E3EA o00167-1 Eyes absent homolog 2 EYA2 EAB1 protein protein human Homo sapiens Human MVELVISPSLTVNSDCLDKLKFNRADAAVWTLSDRQGITKSAPLRVSQLFSRSCPRVLPRQPSTAMAAYGQTQYSAGIQQATPYTAYPPPAQAYGIPSYSIKTEDSLNHSPGQSGFLSYGSSFSTSPTGQSPYTYQMHGTTGFYQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPAKEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLDETIIIFHSLLTGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFNDLEDCDQIHVDDVSSDDNGQDLSTYNFSADGFHSSAPGANLCLGSGVHGGVDWMRKLAFRYRRVKEMYNTYKNNVGGLIGTPKRETWLQLRAELEALTDLWLTHSLKALNLINSRPNCVNVLVTTTQLIPALAKVLLYGLGSVFPIENIYSATKTGKESCFERIMQRFGRKAVYVVIGDGVEEEQGAKKHNMPFWRISCHADLEALRHALELEYL DIP protein O00167-1 original sequence version: 124 eya2-six1-2 3 4 unspecified role unspecified role unspecified role unspecified role Region 1-189 Region 1-189 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 253-538 Region 253-538 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction eya2-six1-3 1 4 unspecified role unspecified role unspecified role unspecified role Region 1-189 Region 1-189 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q15475:p.Cys16Arg C16R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain C R This feature has been corrected as a result of our quality control procedures. The original label was 'C16R'. Wed May 02 18:12:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys16arg' Tue Jun 30 16:18:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys16Arg' ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 253-538 Region 253-538 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction eya2-six1-4 2 4 unspecified role unspecified role unspecified role unspecified role Region 1-189 Region 1-189 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 253-538 Region 253-538 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O00167-1:p.Ala532Arg A532R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain A R This feature has been corrected as a result of our quality control procedures. The original label was 'A532R'. Wed May 02 15:37:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala532arg' Tue Jun 30 17:56:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala532Arg' O00167-1:p.Pro516Arg P516R mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain P R This feature has been corrected as a result of our quality control procedures. The original label was 'P516R'. Wed May 02 16:02:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro516arg' Tue Jun 30 19:17:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro516Arg' ecolx Escherichia coli direct interaction direct interaction