IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structure and interactions of the human programmed cell death 1 receptor. Structure and interactions of the human programmed cell death 1 receptor. The Journal of biological chemistry 2013 imex curation Cheng X., Veverka V., Radhakrishnan A., Waters LC., Muskett FW., Morgan SH., Huo J., Yu C., Evans EJ., Leslie AJ., Griffiths M., Stubberfield C., Griffin R., Henry AJ., Jansson A., Ladbury JE., Ikemizu S., Carr MD., Davis SJ. mdc12@le.ac.uk,simon.davis@imm.ox.ac.uk 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Cheng X., Veverka V., Radhakrishnan A., Waters LC., Muskett FW., Morgan SH., Huo J., Yu C., Evans EJ., Leslie AJ., Griffiths M., Stubberfield C., Griffin R., Henry AJ., Jansson A., Ladbury JE., Ikemizu S., Carr MD., Davis SJ. mdc12@le.ac.uk,simon.davis@imm.ox.ac.uk Accepted 2018-MAY-23 AT 09:49 BST AT 09:49 BST by PPORRAS The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation I have put each interaction in a separate experiment - as I think this is what we are meant to do. (however the graph does not look as I would have thought it would - so maybe not?). Also I have assumed the defaults are:- pH 7.4 and 25 °C in a buffer containing 150 mm NaCl. (I also assume we are not meant to convert degrees C to degrees K= T(K) = T(°C) + 273.15- asonly degrees K is on menu) Structure and interactions of the human programmed cell death 1 receptor. Structure and interactions of the human programmed cell death 1 receptor. The Journal of biological chemistry 2013 imex curation Cheng X., Veverka V., Radhakrishnan A., Waters LC., Muskett FW., Morgan SH., Huo J., Yu C., Evans EJ., Leslie AJ., Griffiths M., Stubberfield C., Griffin R., Henry AJ., Jansson A., Ladbury JE., Ikemizu S., Carr MD., Davis SJ. mdc12@le.ac.uk,simon.davis@imm.ox.ac.uk 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined pH 7.4 6.0 8.0 Termperature in deg.C 10 15 20 25 [NaCl] mM 150 50 500 Cheng X., Veverka V., Radhakrishnan A., Waters LC., Muskett FW., Morgan SH., Huo J., Yu C., Evans EJ., Leslie AJ., Griffiths M., Stubberfield C., Griffin R., Henry AJ., Jansson A., Ladbury JE., Ikemizu S., Carr MD., Davis SJ. mdc12@le.ac.uk,simon.davis@imm.ox.ac.uk Accepted 2018-MAY-23 AT 09:49 BST AT 09:49 BST by PPORRAS The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation I have put each interaction in a separate experiment - as I think this is what we are meant to do. (however the graph does not look as I would have thought it would - so maybe not?). Also I have assumed the defaults are:- pH 7.4 and 25 °C in a buffer containing 150 mm NaCl. (I also assume we are not meant to convert degrees C to degrees K= T(K) = T(°C) + 273.15- asonly degrees K is on menu) Structure and interactions of the human programmed cell death 1 receptor. Structure and interactions of the human programmed cell death 1 receptor. The Journal of biological chemistry 2013 imex curation Cheng X., Veverka V., Radhakrishnan A., Waters LC., Muskett FW., Morgan SH., Huo J., Yu C., Evans EJ., Leslie AJ., Griffiths M., Stubberfield C., Griffin R., Henry AJ., Jansson A., Ladbury JE., Ikemizu S., Carr MD., Davis SJ. mdc12@le.ac.uk,simon.davis@imm.ox.ac.uk 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined pH 6.0 7.4 8.0 Termperature in deg.C 10 15 20 25 [NaCl] mM 150 50 500 Cheng X., Veverka V., Radhakrishnan A., Waters LC., Muskett FW., Morgan SH., Huo J., Yu C., Evans EJ., Leslie AJ., Griffiths M., Stubberfield C., Griffin R., Henry AJ., Jansson A., Ladbury JE., Ikemizu S., Carr MD., Davis SJ. mdc12@le.ac.uk,simon.davis@imm.ox.ac.uk Accepted 2018-MAY-23 AT 09:49 BST AT 09:49 BST by PPORRAS The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation I have put each interaction in a separate experiment - as I think this is what we are meant to do. (however the graph does not look as I would have thought it would - so maybe not?). Also I have assumed the defaults are:- pH 7.4 and 25 °C in a buffer containing 150 mm NaCl. (I also assume we are not meant to convert degrees C to degrees K= T(K) = T(°C) + 273.15- asonly degrees K is on menu) Structure and interactions of the human programmed cell death 1 receptor. Structure and interactions of the human programmed cell death 1 receptor. The Journal of biological chemistry 2013 imex curation Cheng X., Veverka V., Radhakrishnan A., Waters LC., Muskett FW., Morgan SH., Huo J., Yu C., Evans EJ., Leslie AJ., Griffiths M., Stubberfield C., Griffin R., Henry AJ., Jansson A., Ladbury JE., Ikemizu S., Carr MD., Davis SJ. mdc12@le.ac.uk,simon.davis@imm.ox.ac.uk 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Cheng X., Veverka V., Radhakrishnan A., Waters LC., Muskett FW., Morgan SH., Huo J., Yu C., Evans EJ., Leslie AJ., Griffiths M., Stubberfield C., Griffin R., Henry AJ., Jansson A., Ladbury JE., Ikemizu S., Carr MD., Davis SJ. I think the "1HN" I was asking about is meant to refer to an amide bond PD-L1 and PD-L2: ECD sequence numbering as given in Table S1;these refers to UniProt sequence numbering starting from residue 1 - as determined by residue mapping from fig 4. hPD-1 ECD construct (stated to be residues 14-130) refers to Uniprot Q15116 aas 34-150 as detemined by mutation mapping (C73S which maps to C93S -as confirmed by PDBe entry 2M2D). mdc12@le.ac.uk,simon.davis@imm.ox.ac.uk Accepted 2018-MAY-23 AT 09:49 BST AT 09:49 BST by PPORRAS The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation I have put each interaction in a separate experiment - as I think this is what we are meant to do. (however the graph does not look as I would have thought it would - so maybe not?). Also I have assumed the defaults are:- pH 7.4 and 25 °C in a buffer containing 150 mm NaCl. (I also assume we are not meant to convert degrees C to degrees K= T(K) = T(°C) + 273.15- asonly degrees K is on menu) pdcd1_mouse Programmed cell death protein 1 Pdcd1 Pd1 protein protein mouse Mus musculus Mouse MWVRQVPWSFTWAVLQLSWQSGWLLEVPNGPWRSLTFYPAWLTVSEGANATFTCSLSNWSEDLMLNWNRLSPSNQTEKQAAFCNGLSQPVQDARFQIIQLPNRHDFHMNILDTRRNDSGIYLCGAISLHPKAKIEESPGAELVVTERILETSTRYPSPSPKPEGRFQGMVIGIMSALVGIPVLLLLAWALAVFCSTSMSEARGAGSKDDTLKEEPSAAPVPSVAYEELDFQGREKTPELPTACVHTEYATIVFTEGLGASAMGRRGSADGLQGPRPPRHEDGHCSWPL DIP protein Q02242 original sequence version: 72 DIP protein Q02242 original sequence version: 75 4AD3C5F0F9D4200A pd1l1_mouse Programmed cell death 1 ligand 1 Cd274 B7h1 Pdcd1l1 Pdcd1lg1 Pdl1 B7 homolog 1 protein protein mouse Mus musculus Mouse MRIFAGIIFTACCHLLRAFTITAPKDLYVVEYGSNVTMECRFPVERELDLLALVVYWEKEDEQVIQFVAGEEDLKPQHSNFRGRASLPKDQLLKGNAALQITDVKLQDAGVYCCIISYGGADYKRITLKVNAPYRKINQRISVDPATSEHELICQAEGYPEAEVIWTNSDHQPVSGKRSVTTSRTEGMLLNVTSSLRVNATANDVFYCTFWRSQPGQNHTAELIIPELPATHPPQNRTHWVLLGSILLFLIVVSTVLLFLRKQVRMLDVEKCGVEDTSSKNRNDTQFEET DIP protein Q9EP73 original sequence version: 51 AB7C46CF853EBB02 cd80_human T-lymphocyte activation antigen CD80 CD80 CD28LG CD28LG1 LAB7 Activation B7-1 antigen CTLA-4 counter-receptor B7.1 BB1 protein protein human Homo sapiens Human MGHTRRQGTSPSKCPYLNFFQLLVLAGLSHFCSGVIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDNLLPSWAITLISVNGIFVICCLTYCFAPRCRERRRNERLRRESVRPV BA453EE34528B1F4 pd1l1_human Programmed cell death 1 ligand 1 CD274 B7H1 PDCD1L1 PDCD1LG1 PDL1 B7 homolog 1 protein protein human Homo sapiens Human MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEET FE957086E62A31A8 pdcd2_mouse Programmed cell death protein 2 Zinc finger protein Rp-8 Pdcd2 Rp8 protein protein mouse Mus musculus Mouse MAAAAPGPVELGFAEEAPAWRLRSEQFPSKVGGRPAWLGLAELPGPGALACARCGRPLAFLLQVYAPLPGRDDAFHRSLFLFCCREPLCCAGLRVFRNQLPRNNAFYSYEPPSETEALGTECVCLQLKSGAHLCRVCGCLAPMTCSRCKQAHYCSKEHQTLDWRLGHKQACTQSDKIDHMVPDHNFLFPEFEIVTETEDEILPEVVEMEDYSEVTGSMGGIPEEELDSMAKHESKEDHIFQKFKSKIALEPEQILRYGRGIKPIWISGENIPQEKDIPDCPCGAKRIFEFQVMPQLLNHLKADRLGRSIDWGVLAVFTCAESCSLGSGYTEEFVWKQDVTDTP 6D67243B0EA16BAC pd1l2_human Programmed cell death 1 ligand 2 Butyrophilin B7-DC PDCD1LG2 B7DC CD273 PDCD1L2 PDL2 protein protein human Homo sapiens Human MIFLLLMLSLELQLHQIAALFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHPTWLLHIFIPFCIIAFIFIATVIALRKQLCQKLYSSKDTTKRPVTTTKREVNSAI 8B7E963C9BA26ED9 cd80_mouse T-lymphocyte activation antigen CD80 Cd80 B7 Activation B7-1 antigen protein protein mouse Mus musculus Mouse MACNCQLMQDTPLLKFPCPRLILLFVLLIRLSQVSSDVDEQLSKSVKDKVLLPCRYNSPHEDESEDRIYWQKHDKVVLSVIAGKLKVWPEYKNRTLYDNTTYSLIILGLVLSDRGTYSCVVQKKERGTYEVKHLALVKLSIKADFSTPNITESGNPSADTKRITCFASGGFPKPRFSWLENGRELPGINTTISQDPESELYTISSQLDFNTTRNHTIKCLIKYGDAHVSEDFTWEKPPEDPPDSKNTLVLFGAGFGAVITVVVIVVIIKCFCKHRSCFRRNEASRETNNSLTFGPEEALAEQTVFL 1DBADE0931B84C62 pdcd1_human Programmed cell death protein 1 PDCD1 PD1 protein protein human Homo sapiens Human MQIPQAPWPVVWAVLQLGWRPGWFLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQTLVVGVVGGLLGSLVLLVWVLAVICSRAARGTIGARRTGQPLKEDPSAVPVFSVDYGELDFQWREKTPEPPVPCVPEQTEYATIVFPSGMGTSSPARRGSADGPRSAQPLRPEDGHCSWPL DIP protein Q15116 original sequence version: 143 A5210FD40C304FB7 cd274-pdcd1-6 24 32 unspecified role unspecified role neutral component neutral component purified purified region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q15116:p.Cys93Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 20:28:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys93ser' Tue Jun 30 19:13:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys93Ser' region 2h label 2h label deuterium D2 2H2 2H2 deuterium D2 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 28 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 direct interaction direct interaction Figure 2A pdcd1-pdcd1lg2-2 24 32 unspecified role unspecified role neutral component neutral component purified purified region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q15116:p.Cys93Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:48:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys93ser' Tue Jun 30 15:49:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys93Ser' region 2h label 2h label deuterium D2 2H2 2H2 deuterium D2 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 30 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 direct interaction direct interaction Figure 2B cd274-pdcd1-7 1 28 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 32 unspecified role unspecified role prey prey purified purified Q15116:p.Cys93Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:02:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys93ser' Tue Jun 30 17:35:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys93Ser' ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 5 A/B, Fig 7, table 2 cd274-pdcd1-8 1 30 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 32 unspecified role unspecified role prey prey purified purified Q15116:p.Cys93Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 14:48:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys93ser' Tue Jun 30 16:30:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys93Ser' ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 5 C/D, Fig 7, table 2i cd274-pdcd1-9 1 28 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 27 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 direct interaction direct interaction Figure 5 E/F, 7, table 2 cd274-pdcd1-10 1 28 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role bait bait purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Figure 6 A/B, Fig 7, table 2 It is not stated which hPD-1 Fc construct was used nor which expression system. pdcd1-cd274-1 1 30 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role bait bait purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Figure 6 C/D, Fig 7, table 2 It is not stated which hPD-1 Fc construct was used nor which expression system. cd274-cd80-5 1 28 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 27 unspecified role unspecified role bait bait purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. direct interaction direct interaction Table 2 cd80-cd274-2 1 25 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 26 unspecified role unspecified role bait bait purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. direct interaction direct interaction Table 2, fig 8 this is the only mouse PD-1 construct - so must be this one, despite fc tag cd274-pdcd1-11 1 25 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 29 unspecified role unspecified role bait bait purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. direct interaction direct interaction Table 2, fig 8 this is the only mouse PD-1 construct - so must be this one, despite fc tag pdcd2-pdcd1-1 1 31 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 26 unspecified role unspecified role bait bait purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. direct interaction direct interaction Table 2, fig 8 cd274-pdcd1-12 2 28 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 10 9 4 Figure 9 B, table 3i-1 It is not stated which hPD-1 Fc construct was used nor which expression system. Kd values ranged from 1.6 uM at pH 6.0 to 3.04 uM at pH 8.0. A rise in temperature lowered the Kd from 3.38 uM at 10degC to ~1.8 uM at 15-25 degC. pdcd1-pdcd1lg2-5 13 30 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 21 20 14 Figure 9 A/B, table 3-1 It is not stated which hPD-1 Fc construct was used nor which expression system. cd274-pdcd1-14 2 28 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 10 3 9 Figure 9 B, table 3i-2 It is not stated which hPD-1 Fc construct was used nor which expression system. Kd values ranged from 1.6 uM at pH 6.0 to 3.04 uM at pH 8.0. A rise in temperature lowered the Kd from 3.38 uM at 10degC to ~1.8 uM at 15-25 degC. cd274-pdcd1-15 2 28 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 10 9 5 Figure 9 B, table 3i-3 It is not stated which hPD-1 Fc construct was used nor which expression system. Kd values ranged from 1.6 uM at pH 6.0 to 3.04 uM at pH 8.0. A rise in temperature lowered the Kd from 3.38 uM at 10degC to ~1.8 uM at 15-25 degC. cd274-pdcd1-16 2 28 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 6 10 3 Figure 9 B, table 3i-4 It is not stated which hPD-1 Fc construct was used nor which expression system. Kd values ranged from 1.6 uM at pH 6.0 to 3.04 uM at pH 8.0. A rise in temperature lowered the Kd from 3.38 uM at 10degC to ~1.8 uM at 15-25 degC. cd274-pdcd1-17 2 28 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 10 3 7 Figure 9 B, table 3i-5 It is not stated which hPD-1 Fc construct was used nor which expression system. Kd values ranged from 1.6 uM at pH 6.0 to 3.04 uM at pH 8.0. A rise in temperature lowered the Kd from 3.38 uM at 10degC to ~1.8 uM at 15-25 degC. cd274-pdcd1-18 2 28 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 8 10 3 Figure 9 B, table 3i-6 It is not stated which hPD-1 Fc construct was used nor which expression system. Kd values ranged from 1.6 uM at pH 6.0 to 3.04 uM at pH 8.0. A rise in temperature lowered the Kd from 3.38 uM at 10degC to ~1.8 uM at 15-25 degC. cd274-pdcd1-19 2 28 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 10 3 9 Figure 9 B, table 3i-7 It is not stated which hPD-1 Fc construct was used nor which expression system. Kd values ranged from 1.6 uM at pH 6.0 to 3.04 uM at pH 8.0. A rise in temperature lowered the Kd from 3.38 uM at 10degC to ~1.8 uM at 15-25 degC. cd274-pdcd1-20 2 28 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 11 3 9 Figure 9 B, table 3i-8 It is not stated which hPD-1 Fc construct was used nor which expression system. Kd values ranged from 1.6 uM at pH 6.0 to 3.04 uM at pH 8.0. A rise in temperature lowered the Kd from 3.38 uM at 10degC to ~1.8 uM at 15-25 degC. cd274-pdcd1-21 2 28 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 12 3 9 Figure 9 B, table 3i-9 It is not stated which hPD-1 Fc construct was used nor which expression system. Kd values ranged from 1.6 uM at pH 6.0 to 3.04 uM at pH 8.0. A rise in temperature lowered the Kd from 3.38 uM at 10degC to ~1.8 uM at 15-25 degC. pdcd1-pdcd1lg2-6 13 30 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 21 15 20 Figure 9 A/B, table 3-2 It is not stated which hPD-1 Fc construct was used nor which expression system. pdcd1-pdcd1lg2-7 13 30 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 21 20 16 Figure 9 A/B, table 3-3 It is not stated which hPD-1 Fc construct was used nor which expression system. pdcd1-pdcd1lg2-8 13 30 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 17 21 15 Figure 9 A/B, table 3-4 It is not stated which hPD-1 Fc construct was used nor which expression system. pdcd1-pdcd1lg2-9 13 30 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 21 15 18 Figure 9 A/B, table 3-5 It is not stated which hPD-1 Fc construct was used nor which expression system. pdcd1-pdcd1lg2-10 13 30 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 19 21 15 Figure 9 A/B, table 3-6 It is not stated which hPD-1 Fc construct was used nor which expression system. pdcd1-pdcd1lg2-11 13 30 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 21 15 20 Figure 9 A/B, table 3-7 It is not stated which hPD-1 Fc construct was used nor which expression system. pdcd1-pdcd1lg2-12 13 30 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 22 15 20 Figure 9 A/B, table 3-8 It is not stated which hPD-1 Fc construct was used nor which expression system. pdcd1-pdcd1lg2-13 13 30 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 32 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 23 15 20 Figure 9 A/B, table 3-9 It is not stated which hPD-1 Fc construct was used nor which expression system.