DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure (London, England : 1993) 2013 imex curation Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. DIP original experiment: DIP-252342X Structure (London, England : 1993) 2013 IMEx Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure (London, England : 1993) 2013 imex curation Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. DIP original experiment: DIP-252346X Structure (London, England : 1993) 2013 IMEx Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure (London, England : 1993) 2013 imex curation Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. DIP original experiment: DIP-252344X Structure (London, England : 1993) 2013 IMEx Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure (London, England : 1993) 2013 imex curation Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. DIP original experiment: DIP-252343X Structure (London, England : 1993) 2013 IMEx Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure (London, England : 1993) 2013 imex curation Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. DIP original experiment: DIP-252341X Structure (London, England : 1993) 2013 IMEx Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure (London, England : 1993) 2013 imex curation Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. Only protein-protein interactions in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. DIP original experiment: DIP-252347X Structure (London, England : 1993) 2013 IMEx Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure (London, England : 1993) 2013 imex curation Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang W., Maucuer A., Gupta A., Manceau V., Thickman KR., Bauer WJ., Kennedy SD., Wedekind JE., Green MR., Kielkopf CL. DIP original experiment: DIP-252345X Structure (London, England : 1993) 2013 IMEx Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. u2af2_human Splicing factor U2AF 65 kDa subunit U2AF2 U2AF65 U2 snRNP auxiliary factor large subunit U2 auxiliary factor 65 kDa subunit protein protein human Homo sapiens Human MSDFDEFERQLNENKQERDKENRHRKRSHSRSRSRDRKRRSRSRDRRNRDQRSASRDRRRRSKPLTRGAKEEHGGLIRSPRHEKKKKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW DIP protein P26368 original sequence version: 86 DIP protein P26368 original sequence version: 151 DIP protein P26368-1 original sequence version: 151 DIP protein P26368 original sequence version: 124 26AD271CD8FC6211 sf01_human Splicing factor 1 Zinc finger protein 162 Transcription factor ZFM1 Zinc finger gene in MEN1 locus Mammalian branch point-binding protein SF1 ZFM1 ZNF162 protein protein human Homo sapiens Human MATGANATPLDFPSKKRKRSRWNQDTMEQKTVIPGMPTVIPPGLTREQERAYIVQLQIEDLTRKLRTGDLGIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERHNLITEMVALNPDFKPPADYKPPATRVSDKVMIPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVKEGKVGRKDGQMLPGEDEPLHALVTANTMENVKKAVEQIRNILKQGIETPEDQNDLRKMQLRELARLNGTLREDDNRILRPWQSSETRSITNTTVCTKCGGAGHIASDCKFQRPGDPQSAQDKARMDKEYLSLMAELGEAPVPASVGSTSGPATTPLASAPRPAAPANNPPPPSLMSTTQSRPPWMNSGPSESRPYHGMHGGGPGGPGGGPHSFPHPLPSLTGGHGGHPMQHNPNGPPPPWMQPPPPPMNQGPHPPGHHGPPPMDQYLGSTPVGSGVYRLHQGKGMMPPPPMGMMPPPPPPPSGQPPPPPSGPLPPWQQQQQQPPPPPPPSSSMASSTPLPWQQNTTTTTTSAGTGSIPPWQQQQAAAAASPGAPQMQGNPTMVPLPPGVQPPLPPGAPPPPPPPPPGSAGMMYAPPPPPPPPMDPSNFVTMMGMGVAGMPPFGMPPAPPPPPPQN DIP protein Q15637 original sequence version: 73 DIP protein Q15637-1 original sequence version: 138 EEBC6A02B29DAE4D sf1-u2af2-9 5 8 unspecified role unspecified role prey prey human Homo sapiens Human 9 unspecified role unspecified role bait bait Region 1-255 Region 1-255 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q15637:p.Trp22Ala W22A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W22A'. Wed May 02 15:24:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp22ala' Tue Jun 30 19:32:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp22Ala' Q15637:p.[Ser80Ala;Ser82Ala] S80A/S82A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S80A/S82A'. Wed May 02 17:40:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser80ala,ser82ala' Tue Jun 30 16:52:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser80Ala;Ser82Ala]' Q15637:p.[Arg93Glu;Arg97Glu;Arg100Glu] R93E/R97E/R100E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R E certain certain sequence position certain certain certain sequence position certain R E certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'R93E/R97E/R100E'. Wed May 02 14:59:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg93glu,arg97glu,arg100glu' Tue Jun 30 19:59:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg93Glu;Arg97Glu;Arg100Glu]' Q15637:p.[Ile40Arg;Ile53Arg] I40R/I53R mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain I R certain certain sequence position certain certain certain sequence position certain I R This feature has been corrected as a result of our quality control procedures. The original label was 'I40R/I53R'. Wed May 02 16:04:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile40arg,ile53arg' Tue Jun 30 17:43:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile40Arg;Ile53Arg]' Q15637:p.Arg46Asp R46D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R D This feature has been corrected as a result of our quality control procedures. The original label was 'R46D'. Wed May 02 20:11:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg46asp' Tue Jun 30 15:45:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg46Asp' GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association sf1-u2af2-10 7 8 unspecified role unspecified role unspecified role unspecified role UHM domain Region 375-475 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role Region 14-132 Region 14-132 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction sf1-u2af2-11 6 8 unspecified role unspecified role unspecified role unspecified role Region 148-475 Region 148-475 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role Region 1-255 Region 1-255 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction sf1-u2af2-12 4 8 unspecified role unspecified role unspecified role unspecified role UHM domain Region 375-475 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role Region 14-132 Region 14-132 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction sf1-u2af2-13 2 8 unspecified role unspecified role unspecified role unspecified role Region 148-475 Region 148-475 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role Region 1-255 Region 1-255 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction sf1-u2af2-14 1 8 unspecified role unspecified role unspecified role unspecified role UHM domain Region 375-475 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role Region 1-255 Region 1-255 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction sf1-u2af2-15 3 8 unspecified role unspecified role unspecified role unspecified role UHM domain Region 375-475 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role Region 14-132 Region 14-132 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction