DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structure (London, England : 1993) 2013 imex curation Bosch DE., Kimple AJ., Manning AJ., Muller RE., Willard FS., Machius M., Rogers SL., Siderovski DP. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Bosch DE., Kimple AJ., Manning AJ., Muller RE., Willard FS., Machius M., Rogers SL., Siderovski DP. DIP original experiment: DIP-251901X Structure (London, England : 1993) 2013 IMEx Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structure (London, England : 1993) 2013 imex curation Bosch DE., Kimple AJ., Manning AJ., Muller RE., Willard FS., Machius M., Rogers SL., Siderovski DP. Only protein-protein interactions in vitro In vitro gtpase assay gtpase assay GTPase gtp hydrolisis gtp hydrolisis GTPase predetermined predetermined participant predetermined Bosch DE., Kimple AJ., Manning AJ., Muller RE., Willard FS., Machius M., Rogers SL., Siderovski DP. DIP original experiment: DIP-251904X Structure (London, England : 1993) 2013 IMEx Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structure (London, England : 1993) 2013 imex curation Bosch DE., Kimple AJ., Manning AJ., Muller RE., Willard FS., Machius M., Rogers SL., Siderovski DP. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Bosch DE., Kimple AJ., Manning AJ., Muller RE., Willard FS., Machius M., Rogers SL., Siderovski DP. DIP original experiment: DIP-251902X Structure (London, England : 1993) 2013 IMEx Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structure (London, England : 1993) 2013 imex curation Bosch DE., Kimple AJ., Manning AJ., Muller RE., Willard FS., Machius M., Rogers SL., Siderovski DP. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Bosch DE., Kimple AJ., Manning AJ., Muller RE., Willard FS., Machius M., Rogers SL., Siderovski DP. DIP original experiment: DIP-251899X Structure (London, England : 1993) 2013 IMEx Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structure (London, England : 1993) 2013 imex curation Bosch DE., Kimple AJ., Manning AJ., Muller RE., Willard FS., Machius M., Rogers SL., Siderovski DP. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Bosch DE., Kimple AJ., Manning AJ., Muller RE., Willard FS., Machius M., Rogers SL., Siderovski DP. DIP original experiment: DIP-251900X Structure (London, England : 1993) 2013 IMEx Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. Structure (London, England : 1993) 2013 imex curation Bosch DE., Kimple AJ., Manning AJ., Muller RE., Willard FS., Machius M., Rogers SL., Siderovski DP. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Bosch DE., Kimple AJ., Manning AJ., Muller RE., Willard FS., Machius M., Rogers SL., Siderovski DP. DIP original experiment: DIP-251903X Structure (London, England : 1993) 2013 IMEx Structural determinants of RGS-RhoGEF signaling critical to Entamoeba histolytica pathogenesis. c4m483_enthi EHI_140350 protein protein enthi Entamoeba histolytica MGCGQSKDNSIDKQIVEMAKSKPITVMLLGSGESGKSTIAKQLKILFGGGFPEQERATHKSSICSNVVTCMRTLIEQSAILNHPMKYQPKSKEFTTEDPVTLPFSPELVGDVEALWADEGIQATYEESAKFQLPDCAKYLFENVKRIAMEDYVPTEEDLIHNRTKTTGIHEYDFVVKDIPFHLIDVGGQRSERKKWVSFFSDVDCAIFVTSLAEYDMKLYEDGNTSRLTESIAVFKDIMTNEFLKGAVKLIFLNKMDLFEEKLTKVPLNTIFPEYTGGDNAVMGAQYIQQLFTGKLQTEEMNISGADGTANIEGAVNEKVYTNPTNATDGSNIKRVFMLAVDVIMKNMAANGKMRPTK DIP protein C4M483 original sequence version: 23 racc_enthi Rho-related protein racC RACC protein protein enthi Entamoeba histolytica MSEKPTSIKLVVVGDGAVGKTCLLICYTTNEFPKDYIPTVFDNYVVSLTAGTRQIQLALWDTAGQEEYDQLRPLSYSSASIFLICFSVTSSVSYDNVITKWHPEVIHFAPKVPIILVGTKLDTRNDPAIVKRLTEQGMTVINTAKGEELKNRIKAVKYIECSAKTSENLKTVFDEAVKTVLMNKPQQRSKCALL DIP protein Q24816 original sequence version: 68 c4lyv4_enthi EHI_010670 protein protein enthi Entamoeba histolytica MAATETAILEGWPTLQEVLEDSFMKRLLRCYLSDERSEENLDFLESVGLYESQFDKLTPKVRLEALNFIKDQFLDRNSERQVNLSYQIQQSILKKLSEVTSNAPKDVFNEAKKATEYLLYTEQYTYFINKLNANTIGTGKKDVYSLYLNQFPKTNPQPLYKPTLNKIIEIEKKSWNEDEVKRNTESIKSLVESLIQDECNYVGVLTSLSEFSEIMTKKQILGPDVLKELFDHIPVLIQHHQKFISSLQEAKADEKVGEKLNSGLHFLVLYRYYLRHVPKNIAKLCSIGMTDEIEVGRELYPLPVIEEFDKQQKMTKKMSVLQMLVYPYFRVRTYQAYVDDFIKITKKDSQEVKELEVVHSQLAIFQELINTYSDINKIERISDALKLLFPFSFTSIMPLFEGKNGICGIASLDRFDKTDINQLSMSLNSRKKLTLIILYRGVVVTDVPVIRKKGNVSNSIDKSFYSFTLIGDIRDFGTEDSTETIYIDVPEIKKRIWFGCESTEEFKSCVEALRTILSN DIP protein C4LYV4 original sequence version: 16 c4m483_enthi-c4lyv4_enthi-1 4 9 unspecified role unspecified role prey prey RGS domain Region 1-152 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 7 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association c4m483_enthi-c4lyv4_enthi-2 1 9 unspecified role unspecified role prey prey C4LYV4:p.Asn83Ala N83A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A This feature has been corrected as a result of our quality control procedures. The original label was 'N83A'. Wed May 02 18:01:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn83ala' Tue Jun 30 16:11:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn83Ala' ecolx Escherichia coli 7 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction c4lyv4_enthi-c4m483_enthi-1 2 9 unspecified role unspecified role unspecified role unspecified role C4LYV4:p.Glu39Lys E39K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E39K'. Wed May 02 15:41:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu39lys' Tue Jun 30 17:32:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu39Lys' C4LYV4:p.Asn83Ala N83A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A This feature has been corrected as a result of our quality control procedures. The original label was 'N83A'. Wed May 02 19:15:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn83ala' Tue Jun 30 19:52:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn83Ala' ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction c4m483_enthi-c4lyv4_enthi-3 3 9 unspecified role unspecified role prey prey ecolx Escherichia coli 7 unspecified role unspecified role bait bait C4M483:p.Gln189Leu Q189L mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Q L This feature has been corrected as a result of our quality control procedures. The original label was 'Q189L'. Wed May 02 17:03:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln189leu' Tue Jun 30 18:28:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln189Leu' C4M483:p.Gly168Ser G168S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G S This feature has been corrected as a result of our quality control procedures. The original label was 'G168S'. Wed May 02 16:02:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly168ser' Tue Jun 30 18:55:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly168Ser' ecolx Escherichia coli direct interaction direct interaction c4lyv4_enthi-racc-1 5 8 unspecified role unspecified role prey prey ecolx Escherichia coli 9 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction c4lyv4_enthi-c4m483_enthi-2 6 9 unspecified role unspecified role bait bait C4LYV4:p.Glu39Lys E39K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E39K'. Wed May 02 18:51:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu39lys' Tue Jun 30 18:22:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu39Lys' ecolx Escherichia coli 7 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction