DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Crag is a GEF for Rab11 required for rhodopsin trafficking and maintenance of adult photoreceptor cells. Crag is a GEF for Rab11 required for rhodopsin trafficking and maintenance of adult photoreceptor cells. PLoS biology 2011 imex curation Xiong B., Bayat V., Jaiswal M., Zhang K., Sandoval H., Charng WL., Li T., David G., Duraine L., Lin YQ., Neely GG., Yamamoto S., Bellen HJ. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xiong B., Bayat V., Jaiswal M., Zhang K., Sandoval H., Charng WL., Li T., David G., Duraine L., Lin YQ., Neely GG., Yamamoto S., Bellen HJ. DIP original experiment: DIP-251366X PLoS biology 2011 IMEx Crag is a GEF for Rab11 required for rhodopsin trafficking and maintenance of adult photoreceptor cells. Crag is a GEF for Rab11 required for rhodopsin trafficking and maintenance of adult photoreceptor cells. Crag is a GEF for Rab11 required for rhodopsin trafficking and maintenance of adult photoreceptor cells. PLoS biology 2011 imex curation Xiong B., Bayat V., Jaiswal M., Zhang K., Sandoval H., Charng WL., Li T., David G., Duraine L., Lin YQ., Neely GG., Yamamoto S., Bellen HJ. Only protein-protein interactions in vitro In vitro gdp_gtp exchange gdp/gtp exchange assay gtp/gdp exchange guanine nucleotide exchange assay gtp/gdp exchange guanine nucleotide exchange assay gdp_gtp exchange predetermined predetermined participant predetermined Xiong B., Bayat V., Jaiswal M., Zhang K., Sandoval H., Charng WL., Li T., David G., Duraine L., Lin YQ., Neely GG., Yamamoto S., Bellen HJ. DIP original experiment: DIP-251367X PLoS biology 2011 IMEx Crag is a GEF for Rab11 required for rhodopsin trafficking and maintenance of adult photoreceptor cells. Crag is a GEF for Rab11 required for rhodopsin trafficking and maintenance of adult photoreceptor cells. Crag is a GEF for Rab11 required for rhodopsin trafficking and maintenance of adult photoreceptor cells. PLoS biology 2011 imex curation Xiong B., Bayat V., Jaiswal M., Zhang K., Sandoval H., Charng WL., Li T., David G., Duraine L., Lin YQ., Neely GG., Yamamoto S., Bellen HJ. Only protein-protein interactions in vitro In vitro gdp_gtp exchange gdp/gtp exchange assay gtp/gdp exchange guanine nucleotide exchange assay gtp/gdp exchange guanine nucleotide exchange assay gdp_gtp exchange predetermined predetermined participant predetermined Xiong B., Bayat V., Jaiswal M., Zhang K., Sandoval H., Charng WL., Li T., David G., Duraine L., Lin YQ., Neely GG., Yamamoto S., Bellen HJ. DIP original experiment: DIP-251368X PLoS biology 2011 IMEx Crag is a GEF for Rab11 required for rhodopsin trafficking and maintenance of adult photoreceptor cells. o18335_drome Rab11 Drab11 CG5771 AAF55850 DRAB11 DRab11 Dm Rab11 DmRab11 Dmel\CG5771 O18335 RAB11 Rab 11 Rab-11 Rab-r11 dRab11 l(3)93Bi l(3)j2D1 rab-11 rab11 rlr7 Dmel_CG5771 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGAREDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIEVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLRELRDHADQNIVIMLVGNKSDLRHLRSVPTDEAKLFAERNGLSFIETSALDSTNVETAFQNILTEIYRIVSQKQIRDPPEGDVIRPSNVEPIDVKPTVTADVRKQCCQ DIP protein O18335 original sequence version: 117 DIP protein O18335 original sequence version: 127 6033C911EFA74D89 o15971_drome AAF50924 DRAB10 DmRab10 Dmel\CG17060 O15971 Rab-r10 rab10 Dmel_CG17060 CG17060 Rab10 DRab10 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAKKTYDLLFKLLLIGDSGVGKTCILFRFSDDAFTSTFISTIGIDFKIKTVELRGKKIKLQIWDTAGQERFHTITTSYYRGAMGIMLVYDITNEKSFENIVKWLRNIDEHANEDVEKMILGNKCDMTDKRVVNKERGEAIAREHGIRFMETSAKSNINIERAFCELAEAILDKTSGRESAENQERVIIDRRNQEKAPGYSKCCA DIP protein O15971 original sequence version: 102 crag_drome DENN domain-containing protein Crag Crag CG12737 Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MEEKRIADYFVVAGMPENPQLLQENSFNDSGRLRAATTIEPITDIGVYFPLLGEEVPEGYEILSHTPTGLQANLNHGSVRTTDCYIYFRRGKDRPPLVDIGVLYDGHERIMSDAEIVAETPGGRVANVNNSSAKTFLTYRRARADMPCNELVVTELCVIVQSKGERAPHAFCLIYKTLNKGYVGSDVYLCYKKSMYRPKHISYKPEILLRYPTFDHTDFPLNLCPSVPLFCLPMGASLEAWPHVNGTEKRKPISPVFSTFVLTVSDGTYKVYGSALTFYEDYDESKLSAEQKELLGWDEEFGAQHSLHMIKAICLLSHHPFGDTFDKWLKYLHRMVLYDVNIPIPVERYITQLLDEVPFPAPSIHLQLSSESNDRILLTQPEDSPLPRSGAGFHILLQNLGTDNCLHVLLLALTEQKILIHSLRPATLTAVAEAIVSLLFPFKWQCPYIPLCPLGLAEVLHAPLPYLIGVDSRFFDLYEPPTDVTCIDLDTNNISLCESQRHLSPKLLPKRAARLLRQTLTELENAKPISYDSTNSLDRDIRKRKRELVLEQRIQEAFLLFMASILRGYRDFLVPISKAPSVGATDPSALFQLKAFLRSRDKSHQKFFELMMKTQMFIRFIEERSFVSDGDHGLSFFDECAEKVGNYDETPAQLHLVDWDTGQNSERTKYIFPPDSVTPAGSQPGGGGGGLAYNYENFTLQPELLQSTKKTALSKFLQLQLNASLSPGSPIARRTKHEIKLSQKMASRCQQHPEAWSKYLLATCYSLYFLILPSMVLDPRHAGKEPEILRAAYDVLVRASRLKITCDEFCYRIMMQLCGIHNLPVLAVRLHYLMKRSGVQANALTYGFYNRCVLESQWPQDSTTISQIRWNRIKNVVLGAAQFRKAGKQRAASKVNKSLSASQDQNLSTLETVDGQSRTSLASSSGDGGGHGLLDFAAFDRLRNKLGSIVRQTVTGGNNETMGDAVNNTGLLIPGELSAPNTPTYGGDTEILAKALQQQQPRKQIMSIGGGDDDDEDEDEDEYTAGSPSTPQKQLEAGADDLEYAGGGDYEADEEDEDEVDEHVAAQRARQRVQSPTKISPRTPVTQNDPLGALNEEESAASATPTQETQQEQQHSQSQIDSSIYSDKPILFRGQRSATFDESTQIGKSMHRSETMPVASSGVTNSLANIGSSLKFTFGRYSPARLSLKKDLKLPANIIENISSISPSLTGKKSNELIQGSLSSIKSAANSLTKKFDEIKGVISANSTPTKTNNGHHPHGLHHGHHHPHHHHHHHSQHGNAEQEEHDAAVHEEGKLRRVSSDLDPWGRLSESRKSSYNNLVPLGENSSTGALHMHAFPAVPDNLYSLTSENAADRDCDVLIQLTTCSQCHNCSVLVYDEEIMSGWTAEDSNLNTTCHACNKLTVPFLSVQIERQVEESEQSDPLQDGKEQIANGNSHKTSLTVPYLNPLVLRKELENILTQEGDIALIKPEFVDEHPIIYWNLLWLMERIESKTHLPELCLPVPSDKEHIDPLSKVKTVHIQCLWDNLSLHTEASGPPMYLLYRETQPTSPLIKALLTDQAQLNKNVIQQIISAIRCNDFATPLKRLANERHKLKSNGVERSHSFYRDILFLALTAIGRSNVDLATFHREYAAVFDKLTERECNMYYRNQDLPPSASTIFCRAYFRPLLLP DIP protein O96957 original sequence version: 63 1C06079C01DCB55B rab11-crag-1 1 6 unspecified role unspecified role prey prey DENN domain Region 189-624 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 4 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association crag-rab10-1 3 5 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper 6 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction crag-rab11-1 2 6 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper 4 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction