DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. Nature structural & molecular biology 2013 imex curation Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. DIP original experiment: DIP-251966X Nature structural & molecular biology 2013 IMEx HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. Nature structural & molecular biology 2013 imex curation Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. DIP original experiment: DIP-251969X Nature structural & molecular biology 2013 IMEx HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. Nature structural & molecular biology 2013 imex curation Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. DIP original experiment: DIP-251967X Nature structural & molecular biology 2013 IMEx HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. Nature structural & molecular biology 2013 imex curation Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. DIP original experiment: DIP-251965X Nature structural & molecular biology 2013 IMEx HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. Nature structural & molecular biology 2013 imex curation Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. DIP original experiment: DIP-251968X Nature structural & molecular biology 2013 IMEx HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. Nature structural & molecular biology 2013 imex curation Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Guce AI., Mortimer SE., Yoon T., Painter CA., Jiang W., Mellins ED., Stern LJ. DIP original experiment: DIP-251964X Nature structural & molecular biology 2013 IMEx HLA-DO acts as a substrate mimic to inhibit HLA-DM by a competitive mechanism. dob_human HLA class II histocompatibility antigen, DO beta chain HLA-DOB MHC class II antigen DOB protein protein human Homo sapiens Human MGSGWVPWVVALLVNLTRLDSSMTQGTDSPEDFVIQAKADCYFTNGTEKVQFVVRFIFNLEEYVRFDSDVGMFVALTKLGQPDAEQWNSRLDLLERSRQAVDGVCRHNYRLGAPFTVGRKVQPEVTVYPERTPLLHQHNLLHCSVTGFYPGDIKIKWFLNGQEERAGVMSTGPIRNGDWTFQTVVMLEMTPELGHVYTCLVDHSSLLSPVSVEWRAQSEYSWRKMLSGIAAFLLGLIFLLVGIVIQLRAQKGYVRTQMSGNEVSRAVLLPQSC DIP protein P13765 original sequence version: 130 dmb_human HLA class II histocompatibility antigen, DM beta chain HLA-DMB DMB RING7 MHC class II antigen DMB Really interesting new gene 7 protein protein protein human Homo sapiens Human MITFLPLLLGLSLGCTGAGGFVAHVESTCLLDDAGTPKDFTYCISFNKDLLTCWDPEENKMAPCEFGVLNSLANVLSQHLNQKDTLMQRLRNGLQNCATHTQPFWGSLTNRTRPPSVQVAKTTPFNTREPVMLACYVWGFYPAEVTITWRKNGKLVMPHSSAHKTAQPNGDWTYQTLSHLALTPSYGDTYTCVVEHIGAPEPILRDWTPGLSPMQTLKVSVSAVTLGLGLIIFSLGVISWRRAGHSSYTPLPGSNYSEGWHIS DIP protein P28068 original sequence version: 131 9B50F6CC22B3A4BA dma_human HLA class II histocompatibility antigen, DM alpha chain HLA-DMA DMA RING6 MHC class II antigen DMA Really interesting new gene 6 protein protein protein human Homo sapiens Human MGHEQNQGAALLQMLPLLWLLPHSWAVPEAPTPMWPDDLQNHTFLHTVYCQDGSPSVGLSEAYDEDQLFFFDFSQNTRVPRLPEFADWAQEQGDAPAILFDKEFCEWMIQQIGPKLDGKIPVSRGFPIAEVFTLKPLEFGKPNTLVCFVSNLFPPMLTVNWHDHSVPVEGFGPTFVSAVDGLSFQAFSYLNFTPEPSDIFSCIVTHEIDRYTAIAYWVPRNALPSDLLENVLCGVAFGLGVLGIIVGIVLIIYFRKPCSGD doa_human HLA class II histocompatibility antigen, DO alpha chain HLA-DOA HLA-DNA HLA-DZA MHC DN-alpha MHC DZ alpha MHC class II antigen DOA protein protein human Homo sapiens Human MALRAGLVLGFHTLMTLLSPQEAGATKADHMGSYGPAFYQSYGASGQFTHEFDEEQLFSVDLKKSEAVWRLPEFGDFARFDPQGGLAGIAAIKAHLDILVERSNRSRAINVPPRVTVLPKSRVELGQPNILICIVDNIFPPVINITWLRNGQTVTEGVAQTSFYSQPDHLFRKFHYLPFVPSAEDVYDCQVEHWGLDAPLLRHWELQVPIPPPDAMETLVCALGLAIGLVGFLVGTVLIIMGTYVSSVPR DIP protein P06340 original sequence version: 119 FA9482197A02AC85 hla_dma-hla_dmb-2 1 9 unspecified role unspecified role unspecified role unspecified role Region 39-226 Region 39-226 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role unspecified role unspecified role Region 30-218 Region 30-218 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 10 unspecified role unspecified role unspecified role unspecified role Region 27-207 Region 27-207 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role unspecified role unspecified role Region 21-211 Region 21-211 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association hla_doa-hla_dob-1 6 7 unspecified role unspecified role unspecified role unspecified role Region 30-218 Region 30-218 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 10 unspecified role unspecified role unspecified role unspecified role Region 27-207 Region 27-207 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction hla_dma-hla_dmb-3 4 9 unspecified role unspecified role unspecified role unspecified role Region 39-226 Region 39-226 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role unspecified role unspecified role Region 21-211 Region 21-211 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction hla_dma-hla_dmb-4 3 9 unspecified role unspecified role unspecified role unspecified role Region 39-226 Region 39-226 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role unspecified role unspecified role Region 30-218 Region 30-218 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 10 unspecified role unspecified role unspecified role unspecified role Region 27-207 Region 27-207 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role unspecified role unspecified role Region 21-211 Region 21-211 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction hla_dma-hla_dmb-5 5 9 unspecified role unspecified role bait bait Region 39-226 Region 39-226 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role prey prey Region 30-218 Region 30-218 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 10 unspecified role unspecified role prey prey Region 27-207 Region 27-207 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role prey prey Region 21-211 Region 21-211 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly association association hla_dma-hla_dmb-6 2 9 unspecified role unspecified role bait bait Region 39-226 Region 39-226 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role prey prey Region 21-211 Region 21-211 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association