DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Nature structural & molecular biology 2013 imex curation Leidig C., Bange G., Kopp J., Amlacher S., Aravind A., Wickles S., Witte G., Hurt E., Beckmann R., Sinning I. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Leidig C., Bange G., Kopp J., Amlacher S., Aravind A., Wickles S., Witte G., Hurt E., Beckmann R., Sinning I. DIP original experiment: DIP-251955X Nature structural & molecular biology 2013 IMEx Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Nature structural & molecular biology 2013 imex curation Leidig C., Bange G., Kopp J., Amlacher S., Aravind A., Wickles S., Witte G., Hurt E., Beckmann R., Sinning I. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Leidig C., Bange G., Kopp J., Amlacher S., Aravind A., Wickles S., Witte G., Hurt E., Beckmann R., Sinning I. DIP original experiment: DIP-251953X Nature structural & molecular biology 2013 IMEx Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Nature structural & molecular biology 2013 imex curation Leidig C., Bange G., Kopp J., Amlacher S., Aravind A., Wickles S., Witte G., Hurt E., Beckmann R., Sinning I. Only protein-protein interactions ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Leidig C., Bange G., Kopp J., Amlacher S., Aravind A., Wickles S., Witte G., Hurt E., Beckmann R., Sinning I. DIP original experiment: DIP-251956X Nature structural & molecular biology 2013 IMEx Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Nature structural & molecular biology 2013 imex curation Leidig C., Bange G., Kopp J., Amlacher S., Aravind A., Wickles S., Witte G., Hurt E., Beckmann R., Sinning I. Only protein-protein interactions ecolx Escherichia coli ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Leidig C., Bange G., Kopp J., Amlacher S., Aravind A., Wickles S., Witte G., Hurt E., Beckmann R., Sinning I. DIP original experiment: DIP-251957X Nature structural & molecular biology 2013 IMEx Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Nature structural & molecular biology 2013 imex curation Leidig C., Bange G., Kopp J., Amlacher S., Aravind A., Wickles S., Witte G., Hurt E., Beckmann R., Sinning I. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Leidig C., Bange G., Kopp J., Amlacher S., Aravind A., Wickles S., Witte G., Hurt E., Beckmann R., Sinning I. DIP original experiment: DIP-251952X Nature structural & molecular biology 2013 IMEx Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. Nature structural & molecular biology 2013 imex curation Leidig C., Bange G., Kopp J., Amlacher S., Aravind A., Wickles S., Witte G., Hurt E., Beckmann R., Sinning I. Only protein-protein interactions ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Leidig C., Bange G., Kopp J., Amlacher S., Aravind A., Wickles S., Witte G., Hurt E., Beckmann R., Sinning I. DIP original experiment: DIP-251954X Nature structural & molecular biology 2013 IMEx Structural characterization of a eukaryotic chaperone--the ribosome-associated complex. g0ryd6_chatd CTHT_0006310 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MNATVVSLPLPTLPEGWAAEKDFKAIGKLTQEGSSMRTLEPVGPHFLAHARRVRHKRTFSEDDRIQAQAKAKKIEQDSDSDISEEEDPMLLARDAKDWKTQDHYKVLGLSKYRWRATEDQIKRAHRKKVLKHHPDKKAAAGRADDDNFFKCIQKAAEILLDPVKRRQFDSVDEEADIPPPTKKQLAKGNYYELWGKVFENEARFSKIQPVPGFGNENSTREEVEAFYNFWYNFDSWRSFEYLDEDVPDDNENRDQKRHMERKNANARKKKKAEDNARLRKLVEEAQAGDERIKRFRQEANAAKNKKRLEREAAERKAAEEARLKKEAEERARKEAEEKAKAEREAAKKAKEAAKNAVKKNKRVLRGSVKEANYFVEGEASAATIDAVLNDVDLVITKIDADEIAALAGKLNGLTVADEIKNVWKEEVSRLVGAGKLKEGDIKALVA DIP protein G0RYD6 original sequence version: 6 g0rzx9_chatd CTHT_0008010 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MAESASKAAPGERVVIGITFGNSNSSIAHTVDDKAEVIANEDGDRQIPTILSYVDGDEYYGQQAKNFLVRNPKNTVAYFRDILGQDFKSVDPTHNHASAHPQEAGDNVVFTIKDKAEEDAEPSTLTVSEIATRYLRRLVGAASEYLGKKVTSAVITIPTNFTEKQKAALIAAAAAADLEVLQLISEPAAAVLAYDARPEATISDKIIVVADLGGSRSDVTVLASRSGMYTILATVHDYEYHGIALDKVLIDHFSKEFLKKNPGAKDPRENPRSLAKLRLEAESTKRALSRSTNASFSVESLIDGLDFASTINRLRYETIARTVFEGFNRLVESAVKKAGLDPLDVDEVIMSGGTSNTPRIAANFRYIFPESTRILAPSTDPSALNPSELQARGAALQASLIQEFETEDIEQSTHAAVTTMPHVTNAIGVVSVSESGEEKFVPIIAPETAVPARRTVHLDAPKEGGDVLVKVVEGSTHINVIKPEPKAKEDGETKEKTEDADDDGDFDDDDEEEEEEEEEEEKREKVWKIGSTLAEAAVRGVKKGAKVEVTINVNTDLTVIVTAREVGGKGGVRGTLSA DIP protein G0RZX9 original sequence version: 5 zuo1_yeast Zuotin Ribosome-associated complex subunit ZUO1 DnaJ-related protein ZUO1 Heat shock protein 40 homolog ZUO1 ZUO1 YGR285C protein protein yeast Saccharomyces cerevisiae Baker's yeast MFSLPTLTSDITVEVNSSATKTPFVRRPVEPVGKFFLQHAQRTLRNHTWSEFERIEAEKNVKTVDESNVDPDELLFDTELADEDLLTHDARDWKTADLYAAMGLSKLRFRATESQIIKAHRKQVVKYHPDKQSAAGGSLDQDGFFKIIQKAFETLTDSNKRAQYDSCDFVADVPPPKKGTDYDFYEAWGPVFEAEARFSKKTPIPSLGNKDSSKKEVEQFYAFWHRFDSWRTFEFLDEDVPDDSSNRDHKRYIERKNKAARDKKKTADNARLVKLVERAVSEDPRIKMFKEEEKKEKERRKWEREAGARAEAEAKAKAEAEAKAKAESEAKANASAKADKKKAKEAAKAAKKKNKRAIRNSAKEADYFGDADKATTIDEQVGLIVDSLNDEELVSTADKIKANAAGAKEVLKESAKTIVDSGKLPSSLLSYFV 0AA76BC11D3C7DAB 0AA76BC11D3C7DAB ssz1_yeast Ribosome-associated complex subunit SSZ1 YHR064C SSZ1 PDR13 Heat shock protein 70 homolog SSZ1 DnaK-related protein SSZ1 Pleiotropic drug resistance protein 13 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSPVIGITFGNTSSSIAYINPKNDVDVIANPDGERAIPSALSYVGEDEYHGGQALQQLIRNPKNTIINFRDFIGLPFDKCDVSKCANGAPAVEVDGKVGFVISRGEGKEEKLTVDEVVSRHLNRLKLAAEDYIGSAVKEAVLTVPTNFSEEQKTALKASAAKIGLQIVQFINEPSAALLAHAEQFPFEKDVNVVVADFGGIRSDAAVIAVRNGIFTILATAHDLSLGGDNLDTELVEYFASEFQKKYQANPRKNARSLAKLKANSSITKKTLSNATSATISIDSLADGFDYHASINRMRYELVANKVFAQFSSFVDSVIAKAELDPLDIDAVLLTGGVSFTPKLTTNLEYTLPESVEILGPQNKNASNNPNELAASGAALQARLISDYDADELAEALQPVIVNTPHLKKPIGLIGAKGEFHPVLLAETSFPVQKKLTLKQAKGDFLIGVYEGDHHIEEKTLEPIPKEENAEEDDESEWSDDEPEVVREKLYTLGTKLMELGIKNANGVEIIFNINKDGALRVTARDLKTGNAVKGEL 07B1A409B213E0B4 07B1A409B213E0B4 scssz1-sczuo1-1 4 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction g0rzx9_chatd-g0ryd6_chatd-1 5 8 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 7 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association scssz1-sczuo1-2 1 9 unspecified role unspecified role unspecified role unspecified role Region 1-110 Region 1-110 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction g0ryd6_chatd-g0rzx9_chatd-1 2 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction scssz1-sczuo1-3 3 9 unspecified role unspecified role unspecified role unspecified role Region 1-110 Region 1-110 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction g0ryd6_chatd-g0rzx9_chatd-2 6 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction