DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces. Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces. Nature structural & molecular biology 2012 imex curation Seyffer F., Kummer E., Oguchi Y., Winkler J., Kumar M., Zahn R., Sourjik V., Bukau B., Mogk A. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Seyffer F., Kummer E., Oguchi Y., Winkler J., Kumar M., Zahn R., Sourjik V., Bukau B., Mogk A. DIP original experiment: DIP-251446X Nature structural & molecular biology 2012 IMEx Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces. Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces. Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces. Nature structural & molecular biology 2012 imex curation Seyffer F., Kummer E., Oguchi Y., Winkler J., Kumar M., Zahn R., Sourjik V., Bukau B., Mogk A. Only protein-protein interactions ecolx Escherichia coli fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Seyffer F., Kummer E., Oguchi Y., Winkler J., Kumar M., Zahn R., Sourjik V., Bukau B., Mogk A. DIP original experiment: DIP-251448X Nature structural & molecular biology 2012 IMEx Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces. Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces. Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces. Nature structural & molecular biology 2012 imex curation Seyffer F., Kummer E., Oguchi Y., Winkler J., Kumar M., Zahn R., Sourjik V., Bukau B., Mogk A. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Seyffer F., Kummer E., Oguchi Y., Winkler J., Kumar M., Zahn R., Sourjik V., Bukau B., Mogk A. DIP original experiment: DIP-251444X Nature structural & molecular biology 2012 IMEx Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces. Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces. Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces. Nature structural & molecular biology 2012 imex curation Seyffer F., Kummer E., Oguchi Y., Winkler J., Kumar M., Zahn R., Sourjik V., Bukau B., Mogk A. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Seyffer F., Kummer E., Oguchi Y., Winkler J., Kumar M., Zahn R., Sourjik V., Bukau B., Mogk A. DIP original experiment: DIP-251445X Nature structural & molecular biology 2012 IMEx Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces. Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces. Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces. Nature structural & molecular biology 2012 imex curation Seyffer F., Kummer E., Oguchi Y., Winkler J., Kumar M., Zahn R., Sourjik V., Bukau B., Mogk A. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Seyffer F., Kummer E., Oguchi Y., Winkler J., Kumar M., Zahn R., Sourjik V., Bukau B., Mogk A. DIP original experiment: DIP-251447X Nature structural & molecular biology 2012 IMEx Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces. clpb_ecoli Chaperone protein ClpB clpB htpM b2592 Heat shock protein F84.1 JW2573 protein protein ecoli Escherichia coli (strain K12) MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGGSVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRGDNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQALKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILFIDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQKVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSDKERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPELEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQELHHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFDSDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKELVLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDEALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAVQ DIP protein P63284 original sequence version: 59 DIP protein P63284-1 original sequence version: 76 DIP protein P63284 original sequence version: 95 DIP protein P63284 original sequence version: 90 FD38CD96B2F7C32A dnak_ecoli Chaperone protein DnaK dnaK groP grpF seg b0014 Heat shock protein 70 Heat shock 70 kDa protein HSP70 JW0013 protein protein ecoli Escherichia coli (strain K12) MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAWVEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDIDDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLLDVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAADNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLNEDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIESALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDDDVVDAEFEEVKDKK DIP protein P0A6Y8 original sequence version: 44 DIP protein P0A6Y8 original sequence version: 96 DIP protein P0A6Y8 original sequence version: 91 DIP protein P0A6Y8 original sequence version: 119 DIP protein P0A6Y8 original sequence version: 95 5A8589B21D7CD9C1 clpb-dnak-2 3 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role P63284:p.Gln481Cys Q481C mutation mutation certain certain sequence position certain certain certain sequence position certain Q C This feature has been corrected as a result of our quality control procedures. The original label was 'Q481C'. Wed May 02 20:04:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln481cys' Tue Jun 30 17:34:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln481Cys' ecolx Escherichia coli direct interaction direct interaction clpb-dnak-3 5 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role P63284:p.Glu500Cys E500C mutation mutation certain certain sequence position certain certain certain sequence position certain E C This feature has been corrected as a result of our quality control procedures. The original label was 'E500C'. Wed May 02 14:59:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu500cys' Tue Jun 30 15:43:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu500Cys' ecolx Escherichia coli direct interaction direct interaction clpb-dnak-4 4 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role P63284:p.Lys483Cys K483C mutation mutation certain certain sequence position certain certain certain sequence position certain K C This feature has been corrected as a result of our quality control procedures. The original label was 'K483C'. Wed May 02 14:43:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys483cys' Tue Jun 30 17:17:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys483Cys' ecolx Escherichia coli direct interaction direct interaction clpb-dnak-5 2 7 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 6 unspecified role unspecified role fluorescence donor fluorescence donor P63284:p.Tyr503Asp Y503D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y D This feature has been corrected as a result of our quality control procedures. The original label was 'Y503D'. Wed May 02 14:31:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr503asp' Tue Jun 30 15:53:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr503Asp' CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P63284:p.Glu432Ala E432A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E432A'. Wed May 02 15:53:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu432ala' Tue Jun 30 16:42:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu432Ala' P63284:p.Lys476Cys K476C mutation increasing strength mutation increasing interaction strength mutation increasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K C Wed May 02 16:39:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys476cys' Tue Jun 30 19:53:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys476Cys' P63284:p.Glu480Cys E480C mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E C This feature has been corrected as a result of our quality control procedures. The original label was 'E480C'. Wed May 02 19:12:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu480cys' Tue Jun 30 18:15:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu480Cys' ecolx Escherichia coli proximity proximity close-contact colocalization neighbourhood interaction clpb-dnak-6 1 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role P63284:p.Gln488Cys Q488C mutation mutation certain certain sequence position certain certain certain sequence position certain Q C This feature has been corrected as a result of our quality control procedures. The original label was 'Q488C'. Wed May 02 18:37:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln488cys' Tue Jun 30 17:05:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln488Cys' ecolx Escherichia coli direct interaction direct interaction