DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural insight into the type-II mitochondrial NADH dehydrogenases. Structural insight into the type-II mitochondrial NADH dehydrogenases. Nature 2012 imex curation Feng Y., Li W., Li J., Wang J., Ge J., Xu D., Liu Y., Wu K., Zeng Q., Wu JW., Tian C., Zhou B., Yang M. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Feng Y., Li W., Li J., Wang J., Ge J., Xu D., Liu Y., Wu K., Zeng Q., Wu JW., Tian C., Zhou B., Yang M. DIP original experiment: DIP-251212X Nature 2012 IMEx Structural insight into the type-II mitochondrial NADH dehydrogenases. Structural insight into the type-II mitochondrial NADH dehydrogenases. Structural insight into the type-II mitochondrial NADH dehydrogenases. Nature 2012 imex curation Feng Y., Li W., Li J., Wang J., Ge J., Xu D., Liu Y., Wu K., Zeng Q., Wu JW., Tian C., Zhou B., Yang M. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Feng Y., Li W., Li J., Wang J., Ge J., Xu D., Liu Y., Wu K., Zeng Q., Wu JW., Tian C., Zhou B., Yang M. DIP original experiment: DIP-251211X Nature 2012 IMEx Structural insight into the type-II mitochondrial NADH dehydrogenases. Structural insight into the type-II mitochondrial NADH dehydrogenases. Structural insight into the type-II mitochondrial NADH dehydrogenases. Nature 2012 imex curation Feng Y., Li W., Li J., Wang J., Ge J., Xu D., Liu Y., Wu K., Zeng Q., Wu JW., Tian C., Zhou B., Yang M. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Feng Y., Li W., Li J., Wang J., Ge J., Xu D., Liu Y., Wu K., Zeng Q., Wu JW., Tian C., Zhou B., Yang M. DIP original experiment: DIP-251213X Nature 2012 IMEx Structural insight into the type-II mitochondrial NADH dehydrogenases. Structural insight into the type-II mitochondrial NADH dehydrogenases. Structural insight into the type-II mitochondrial NADH dehydrogenases. Nature 2012 imex curation Feng Y., Li W., Li J., Wang J., Ge J., Xu D., Liu Y., Wu K., Zeng Q., Wu JW., Tian C., Zhou B., Yang M. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Feng Y., Li W., Li J., Wang J., Ge J., Xu D., Liu Y., Wu K., Zeng Q., Wu JW., Tian C., Zhou B., Yang M. DIP original experiment: DIP-251210X Nature 2012 IMEx Structural insight into the type-II mitochondrial NADH dehydrogenases. ndi1_yeast Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial NDI1 YML120C YM7056.06C NADH:ubiquinone reductase (non-electrogenic) Internal NADH dehydrogenase protein protein yeast Saccharomyces cerevisiae Baker's yeast MLSKNLYSNKRLLTSTNTLVRFASTRSTGVENSGAGPTSFKTMKVIDPQHSDKPNVLILGSGWGAISFLKHIDTKKYNVSIISPRSYFLFTPLLPSAPVGTVDEKSIIEPIVNFALKKKGNVTYYEAEATSINPDRNTVTIKSLSAVSQLYQPENHLGLHQAEPAEIKYDYLISAVGAEPNTFGIPGVTDYGHFLKEIPNSLEIRRTFAANLEKANLLPKGDPERRRLLSIVVVGGGPTGVEAAGELQDYVHQDLRKFLPALAEEVQIHLVEALPIVLNMFEKKLSSYAQSHLENTSIKVHLRTAVAKVEEKQLLAKTKHEDGKITEETIPYGTLIWATGNKARPVITDLFKKIPEQNSSKRGLAVNDFLQVKGSNNIFAIGDNAFAGLPPTAQVAHQEAEYLAKNFDKMAQIPNFQKNLSSRKDKIDLLFEENNFKPFKYNDLGALAYLGSERAIATIRSGKRTFYTGGGLMTFYLWRILYLSMILSARSRLKVFFDWIKLAFFKRDFFKGL E3A43D75A1ADCF3B E3A43D75A1ADCF3B ndi1-ndi1-1 3 5 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 1-488 Region 1-488 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 1-488 Region 1-488 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association ndi1-2 4 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction ndi1-3 2 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction ndi1-ndi1-2 1 5 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association