DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Crystal structure of human CDC7 kinase in complex with its activator DBF4. Crystal structure of human CDC7 kinase in complex with its activator DBF4. Nature structural & molecular biology 2012 imex curation Hughes S., Elustondo F., Di Fonzo A., Leroux FG., Wong AC., Snijders AP., Matthews SJ., Cherepanov P. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hughes S., Elustondo F., Di Fonzo A., Leroux FG., Wong AC., Snijders AP., Matthews SJ., Cherepanov P. DIP original experiment: DIP-251262X Nature structural & molecular biology 2012 IMEx Crystal structure of human CDC7 kinase in complex with its activator DBF4. Crystal structure of human CDC7 kinase in complex with its activator DBF4. Crystal structure of human CDC7 kinase in complex with its activator DBF4. Nature structural & molecular biology 2012 imex curation Hughes S., Elustondo F., Di Fonzo A., Leroux FG., Wong AC., Snijders AP., Matthews SJ., Cherepanov P. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Hughes S., Elustondo F., Di Fonzo A., Leroux FG., Wong AC., Snijders AP., Matthews SJ., Cherepanov P. DIP original experiment: DIP-251260X Nature structural & molecular biology 2012 IMEx Crystal structure of human CDC7 kinase in complex with its activator DBF4. Crystal structure of human CDC7 kinase in complex with its activator DBF4. Crystal structure of human CDC7 kinase in complex with its activator DBF4. Nature structural & molecular biology 2012 imex curation Hughes S., Elustondo F., Di Fonzo A., Leroux FG., Wong AC., Snijders AP., Matthews SJ., Cherepanov P. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hughes S., Elustondo F., Di Fonzo A., Leroux FG., Wong AC., Snijders AP., Matthews SJ., Cherepanov P. DIP original experiment: DIP-251261X Nature structural & molecular biology 2012 IMEx Crystal structure of human CDC7 kinase in complex with its activator DBF4. cdc7_human Cell division cycle 7-related protein kinase CDC7 CDC7L1 protein protein human Homo sapiens Human MEASLGIQMDEPMAFSPQRDRFQAEGSLKKNEQNFKLAGVKKDIEKLYEAVPQLSNVFKIEDKIGEGTFSSVYLATAQLQVGPEEKIALKHLIPTSHPIRIAAELQCLTVAGGQDNVMGVKYCFRKNDHVVIAMPYLEHESFLDILNSLSFQEVREYMLNLFKALKRIHQFGIVHRDVKPSNFLYNRRLKKYALVDFGLAQGTHDTKIELLKFVQSEAQQERCSQNKSHIITGNKIPLSGPVPKELDQQSTTKASVKRPYTNAQIQIKQGKDGKEGSVGLSVQRSVFGERNFNIHSSISHESPAVKLMKQSKTVDVLSRKLATKKKAISTKVMNSAVMRKTASSCPASLTCDCYATDKVCSICLSRRQQVAPRAGTPGFRAPEVLTKCPNQTTAIDMWSAGVIFLSLLSGRYPFYKASDDLTALAQIMTIRGSRETIQAAKTFGKSILCSKEVPAQDLRKLCERLRGMDSSTPKLTSDIQGHASHQPAISEKTDHKASCLVQTPPGQYSGNSFKKGDSNSCEHCFDEYNTNLEGWNEVPDEAYDLLDKLLDLNPASRITAEEALLHPFFKDMSL DIP protein O00311 original sequence version: 127 90D549BEE20AE583 q9ubu7-1 Protein DBF4 homolog A DBF4 ASK DBF4A ZDBF1 Activator of S phase kinase Chiffon homolog A DBF4-type zinc finger-containing protein 1 protein protein human Homo sapiens Human MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQTLGRISPVPSPESAYTAETTSPHPSHDGSSFKSPDTVCLSRGKLLVEKAIKDHDFIPSNSILSNALSWGVKILHIDDIRYYIEQKKKELYLLKKSSTSVRDGGKRVGSGAQKTRTGRLKKPFVKVEDMSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF DIP protein Q9UBU7-1 original sequence version: 95 dbf4-cdc7-1 2 4 unspecified role unspecified role prey prey Region 37-227/360-483/530-574 Region 37-227/360-483/530-574 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role bait bait Region 210-266 Region 210-266 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 288-350 Region 288-350 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli physical association physical association cdc7-dbf4-12 1 4 unspecified role unspecified role unspecified role unspecified role Region 37-227/360-483/530-574 Region 37-227/360-483/530-574 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 210-350 Region 210-350 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cdc7-dbf4-13 3 4 unspecified role unspecified role unspecified role unspecified role Region 37-227/360-483/530-574 Region 37-227/360-483/530-574 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 210-266 Region 210-266 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 288-350 Region 288-350 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction