DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Nature 2012 imex curation Korkhov VM., Mireku SA., Locher KP. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Korkhov VM., Mireku SA., Locher KP. DIP original experiment: DIP-251039X Nature 2012 IMEx Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Nature 2012 imex curation Korkhov VM., Mireku SA., Locher KP. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Korkhov VM., Mireku SA., Locher KP. DIP original experiment: DIP-251036X Nature 2012 IMEx Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Nature 2012 imex curation Korkhov VM., Mireku SA., Locher KP. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Korkhov VM., Mireku SA., Locher KP. DIP original experiment: DIP-251038X Nature 2012 IMEx Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Nature 2012 imex curation Korkhov VM., Mireku SA., Locher KP. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Korkhov VM., Mireku SA., Locher KP. DIP original experiment: DIP-251040X Nature 2012 IMEx Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. Nature 2012 imex curation Korkhov VM., Mireku SA., Locher KP. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Korkhov VM., Mireku SA., Locher KP. DIP original experiment: DIP-251037X Nature 2012 IMEx Structure of AMP-PNP-bound vitamin B12 transporter BtuCD-F. btuf_ecoli Vitamin B12-binding protein yadT btuF b0158 JW0154 protein protein ecoli Escherichia coli (strain K12) MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITPVGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGIKVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFLQFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGPDQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD DIP protein P37028 original sequence version: 92 DIP protein P37028 original sequence version: 126 DIP protein P37028 original sequence version: 110 480F2E620ACD6EA1 btud_ecoli Vitamin B12 import ATP-binding protein BtuD btuD b1709 Vitamin B12-transporting ATPase JW1699 protein protein ecoli Escherichia coli (strain K12) MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLARMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFATPVWHYLTLHQHDKTRTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEPMNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRREEVLTPPNLAQAYGMNFRRLDIEGHRMLISTI DIP protein P06611 original sequence version: 101 DIP protein P06611 original sequence version: 138 DIP protein P06611 original sequence version: 119 760004A5C0134245 btuc_ecoli Vitamin B12 import system permease protein BtuC btuC b1711 JW1701 protein protein ecoli Escherichia coli (strain K12) MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWFTPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLIAAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALMTWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGEISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLLPGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR DIP protein P06609 original sequence version: 96 DIP protein P06609 original sequence version: 131 DIP protein P06609 original sequence version: 114 C65882B1C47BFE69 btud-1 3 7 unspecified role unspecified role unspecified role unspecified role P06611:p.[Glu159Gln;Asn162Cys] E159Q/N162C mutation mutation certain certain sequence position certain certain certain sequence position certain N C certain certain sequence position certain certain certain sequence position certain E Q This feature has been corrected as a result of our quality control procedures. The original label was 'E159Q/N162C'. Wed May 02 20:26:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu159gln,asn162cys' Tue Jun 30 17:18:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu159Gln;Asn162Cys]' ecolx Escherichia coli direct interaction direct interaction btuc-btud-8 2 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association btuc-1 1 8 unspecified role unspecified role unspecified role unspecified role P06609:p.Asn83Cys N83C mutation mutation certain certain sequence position certain certain certain sequence position certain N C This feature has been corrected as a result of our quality control procedures. The original label was 'N83C'. Wed May 02 15:07:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn83cys' Tue Jun 30 19:20:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn83Cys' ecolx Escherichia coli direct interaction direct interaction btuc-btud-9 5 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association btuc-8 4 8 unspecified role unspecified role unspecified role unspecified role P06609:p.Leu85Cys L85C mutation mutation certain certain sequence position certain certain certain sequence position certain L C This feature has been corrected as a result of our quality control procedures. The original label was 'L85C'. Wed May 02 15:13:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu85cys' Tue Jun 30 18:42:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu85Cys' ecolx Escherichia coli direct interaction direct interaction