DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Molecular basis for coupling the plasma membrane to the actin cytoskeleton during clathrin-mediated endocytosis. Molecular basis for coupling the plasma membrane to the actin cytoskeleton during clathrin-mediated endocytosis. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Skruzny M., Brach T., Ciuffa R., Rybina S., Wachsmuth M., Kaksonen M. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Skruzny M., Brach T., Ciuffa R., Rybina S., Wachsmuth M., Kaksonen M. DIP original experiment: DIP-253592X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Molecular basis for coupling the plasma membrane to the actin cytoskeleton during clathrin-mediated endocytosis. Molecular basis for coupling the plasma membrane to the actin cytoskeleton during clathrin-mediated endocytosis. Molecular basis for coupling the plasma membrane to the actin cytoskeleton during clathrin-mediated endocytosis. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Skruzny M., Brach T., Ciuffa R., Rybina S., Wachsmuth M., Kaksonen M. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Skruzny M., Brach T., Ciuffa R., Rybina S., Wachsmuth M., Kaksonen M. DIP original experiment: DIP-253593X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Molecular basis for coupling the plasma membrane to the actin cytoskeleton during clathrin-mediated endocytosis. ent1_yeast Epsin-1 ENT1 YDL161W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSKQFVRSAKNLVKGYSSTQVLVRNATSNDNHQVSKDSLIELAEKSYDSADFFEIMDMLDKRLNDKGKYWRHIAKALTVIDYLIRFGSENCVLWCRENLYIIKTLKEFRHEDDEGIDQGQIVRVKAKELTALLSDDERLNEERNMNIKGRNRKGRRRRGTGRSDENDDDLQRAISASRLTAEEDERRRKQDEDYETALQLSKEEEELKRLQDLQRMQQQQGQQQLQQPMYYDIFGNPITPEEYAQFQLQQQQQQQQQQLQQQPMYYDVFGNPITPEELAQFQQQQQLQEQQYLASMQQQQQAMSNNPFAKSEQSSSSPKRNQLVAASSPQQLQQQKQQEPLIQNRTGNQSMTDKYSKLNELLATGTGIDTFGNVGEARIPAQHTKTGTFINSQGTGYRQVSDDPNHNPFLNSQYTGLPSTSVVPTQTGYGFGNQSQQQSQNNGSNNRGYTLIDL 37447FB4A6449256 37447FB4A6449256 sla2_yeast Protein SLA2 SLA2 END4 MOP2 UFG1 YNL243W N1102 Transmembrane protein MOP2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSRIDSDLQKALKKACSVEETAPKRKHVRACIVYTWDHQSSKAVFTTLKTLPLANDEVQLFKMLIVLHKIIQEGHPSALAEAIRDRDWIRSLGRVHSGGSSYSKLIREYVRYLVLKLDFHAHHRGFNNGTFEYEEYVSLVSVSDPDEGYETILDLMSLQDSLDEFSQIIFASIQSERRNTECKISALIPLIAESYGIYKFITSMLRAMHRQLNDAEGDAALQPLKERYELQHARLFEFYADCSSVKYLTTLVTIPKLPVDAPDVFLINDVDESKEIKFKKREPSVTPARTPARTPTPTPPVVAEPAISPRPVSQRTTSTPTGYLQTMPTGATTGMMIPTATGAANAIFPQATAQMQPDFWANQQAQFANEQNRLEQERVQQLQQQQAQQELFQQQLQKAQQDMMNMQLQQQNQHQNDLIALTNQYEKDQALLQQYDQRVQQLESEITTMDSTASKQLANKDEQLTALQDQLDVWERKYESLAKLYSQLRQEHLNLLPRFKKLQLKVNSAQESIQKKEQLEHKLKQKDLQMAELVKDRDRARLELERSINNAEADSAAATAAAETMTQDKMNPILDAILESGINTIQESVYNLDSPLSWSGPLTPPTFLLSLLESTSENATEFATSFNNLIVDGLAHGDQTEVIHCVSDFSTSMATLVTNSKAYAVTTLPQEQSDQILTLVKRCAREAQYFFEDLMSENLNQVGDEEKTDIVINANVDMQEKLQELSLAIEPLLNIQSVKSNKETNPHSELVATADKIVKSSEHLRVDVPKPLLSLALMIIDAVVALVKAAIQCQNEIATTTSIPLNQFYLKNSRWTEGLISAAKAVAGATNVLITTASKLITSEDNENTSPEQFIVASKEVAASTIQLVAASRVKTSIHSKAQDKLEHCSKDVTDACRSLGNHVMGMIEDDHSTSQQQQPLDFTSEHTLKTAEMEQQVEILKLEQSLSNARKRLGEIRRHAYYNQDDD E592E09D8040C0E9 E592E09D8040C0E9 ent1-sla2-1 1 4 unspecified role unspecified role prey prey ANTH domain Region 1-286 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 3 unspecified role unspecified role bait bait ENTH domain Region 1-154 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q12518:p.Thr104Ala T104A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A This feature has been corrected as a result of our quality control procedures. The original label was 'T104A'. Wed May 02 19:38:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr104ala' Tue Jun 30 18:12:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr104Ala' GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction sla2-ent1-1 2 4 unspecified role unspecified role bait bait ANTH domain Region 1-286 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 3 unspecified role unspecified role prey prey ENTH domain Region 1-154 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q12518:p.Thr104Ala T104A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A This feature has been corrected as a result of our quality control procedures. The original label was 'T104A'. Wed May 02 16:01:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr104ala' Tue Jun 30 15:48:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr104Ala' ecolx Escherichia coli direct interaction direct interaction