DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis. TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis. Nature 2012 imex curation Gregory AP., Dendrou CA., Attfield KE., Haghikia A., Xifara DK., Butter F., Poschmann G., Kaur G., Lambert L., Leach OA., Prömel S., Punwani D., Felce JH., Davis SJ., Gold R., Nielsen FC., Siegel RM., Mann M., Bell JI., McVean G., Fugger L. Only protein-protein interactions human Homo sapiens Human fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Gregory AP., Dendrou CA., Attfield KE., Haghikia A., Xifara DK., Butter F., Poschmann G., Kaur G., Lambert L., Leach OA., Prömel S., Punwani D., Felce JH., Davis SJ., Gold R., Nielsen FC., Siegel RM., Mann M., Bell JI., McVean G., Fugger L. DIP original experiment: DIP-250755X Nature 2012 IMEx TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis. TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis. TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis. Nature 2012 imex curation Gregory AP., Dendrou CA., Attfield KE., Haghikia A., Xifara DK., Butter F., Poschmann G., Kaur G., Lambert L., Leach OA., Prömel S., Punwani D., Felce JH., Davis SJ., Gold R., Nielsen FC., Siegel RM., Mann M., Bell JI., McVean G., Fugger L. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Gregory AP., Dendrou CA., Attfield KE., Haghikia A., Xifara DK., Butter F., Poschmann G., Kaur G., Lambert L., Leach OA., Prömel S., Punwani D., Felce JH., Davis SJ., Gold R., Nielsen FC., Siegel RM., Mann M., Bell JI., McVean G., Fugger L. DIP original experiment: DIP-250757X Nature 2012 IMEx TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis. TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis. TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis. Nature 2012 imex curation Gregory AP., Dendrou CA., Attfield KE., Haghikia A., Xifara DK., Butter F., Poschmann G., Kaur G., Lambert L., Leach OA., Prömel S., Punwani D., Felce JH., Davis SJ., Gold R., Nielsen FC., Siegel RM., Mann M., Bell JI., McVean G., Fugger L. Only protein-protein interactions human Homo sapiens Human bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret predetermined predetermined participant predetermined Gregory AP., Dendrou CA., Attfield KE., Haghikia A., Xifara DK., Butter F., Poschmann G., Kaur G., Lambert L., Leach OA., Prömel S., Punwani D., Felce JH., Davis SJ., Gold R., Nielsen FC., Siegel RM., Mann M., Bell JI., McVean G., Fugger L. DIP original experiment: DIP-250756X Nature 2012 IMEx TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis. p19438-1 Tumor necrosis factor receptor superfamily member 1A Tumor necrosis factor receptor 1 Tumor necrosis factor receptor type I p55 p60 FL-TNFR1 TNFRSF1A TNFAR TNFR1 protein protein human Homo sapiens Human MGLSTVPDLLLPLVLLELLVGIYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWKSKLYSIVCGKSTPEKEGELEGTTTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYTPGDCPNFAAPRREVAPPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTDDPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR DIP protein P19438-1 original sequence version: 135 DIP protein P19438-1 original sequence version: 164 tnfa_human Tumor necrosis factor TNF TNFA TNFSF2 TNF-alpha Tumor necrosis factor ligand superfamily member 2 Cachectin protein protein human Homo sapiens Human MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLFSFLIVAGATTLFCLLHFGVIGPQREEFPRDLSLISPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL DIP protein P01375 original sequence version: 198 DIP protein P01375 original sequence version: 151 DIP protein P01375 original sequence version: 156 DIP protein P01375 original sequence version: 186 DIP protein P01375 original sequence version: 201 DIP protein P01375 original sequence version: 174 DIP protein P01375 original sequence version: 211 3DF90F96C9031FFE tnfrsf1a-1 1 4 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Region 1-232 Region 1-232 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain EYFP-tag EYFP-tag eyfp enhanced yellow fluorescent protein tag eyfp c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 4 unspecified role unspecified role fluorescence donor fluorescence donor ECFP-tag ECFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction tnf-tnfrsf1a-7 2 5 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 4 unspecified role unspecified role prey prey Fc-tag Fc-tag fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human direct interaction direct interaction Deletion of region 1-232 decreased the interaction rate/strength. tnfrsf1a-6 3 4 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 4 unspecified role unspecified role fluorescence donor fluorescence donor RLuc-tag RLuc-tag renilla luciferase renilla luciferase protein tag renilla luciferase c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction