DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu ATP-dependent conformational dynamics underlie the functional asymmetry of the replicative helicase from a minimalist eukaryote. ATP-dependent conformational dynamics underlie the functional asymmetry of the replicative helicase from a minimalist eukaryote. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Lyubimov AY., Costa A., Bleichert F., Botchan MR., Berger JM. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm dls dynamic light scattering dls predetermined predetermined participant predetermined Lyubimov AY., Costa A., Bleichert F., Botchan MR., Berger JM. DIP original experiment: DIP-251722X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx ATP-dependent conformational dynamics underlie the functional asymmetry of the replicative helicase from a minimalist eukaryote. ATP-dependent conformational dynamics underlie the functional asymmetry of the replicative helicase from a minimalist eukaryote. ATP-dependent conformational dynamics underlie the functional asymmetry of the replicative helicase from a minimalist eukaryote. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Lyubimov AY., Costa A., Bleichert F., Botchan MR., Berger JM. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm tem transmission electron microscopy tem predetermined predetermined participant predetermined Lyubimov AY., Costa A., Bleichert F., Botchan MR., Berger JM. DIP original experiment: DIP-251723X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx ATP-dependent conformational dynamics underlie the functional asymmetry of the replicative helicase from a minimalist eukaryote. ATP-dependent conformational dynamics underlie the functional asymmetry of the replicative helicase from a minimalist eukaryote. ATP-dependent conformational dynamics underlie the functional asymmetry of the replicative helicase from a minimalist eukaryote. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Lyubimov AY., Costa A., Bleichert F., Botchan MR., Berger JM. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Lyubimov AY., Costa A., Bleichert F., Botchan MR., Berger JM. DIP original experiment: DIP-251721X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx ATP-dependent conformational dynamics underlie the functional asymmetry of the replicative helicase from a minimalist eukaryote. ATP-dependent conformational dynamics underlie the functional asymmetry of the replicative helicase from a minimalist eukaryote. ATP-dependent conformational dynamics underlie the functional asymmetry of the replicative helicase from a minimalist eukaryote. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Lyubimov AY., Costa A., Bleichert F., Botchan MR., Berger JM. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm saxs x ray scattering saxs predetermined predetermined participant predetermined Lyubimov AY., Costa A., Bleichert F., Botchan MR., Berger JM. DIP original experiment: DIP-251724X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx ATP-dependent conformational dynamics underlie the functional asymmetry of the replicative helicase from a minimalist eukaryote. q8sse5_enccu DNA replication licensing factor MCM4 ECU02_1150 protein protein enccu Encephalitozoon cuniculi (strain GB-M1) Microsporidian parasite MDSDAQSSAFSFYDASQDIQLNLTPSQGDLSSTFTPIVETERVKVIWGTTINIQETAERFKEFVRRNRDYHTILEQMDLTQEFVFDLHCDDLDADLKSQLAMYPQEVLPIFQGSLQEIYMENFPSFGGLIRIRPFGIGRPLSIRNIDPNDIDKIVQISGMVIRSSSVIPEIVRAFFRCSRCGHECLVESVRNVIDEPNKCECGGKYTQQLVHNASEFEDKQVVRIQELPEGIPNGTTPMAMTIVCRNEFVDGLVPGDRVKLTGVLKATPVRLNPVMRKIKSTFRIYLDLLSYQVINRKIESSDPIDKIDELRKRPDVYEILANSVAPSVCGMEDTKKALVLQLFGGVRKELGSSRLRGDINILLAGDPGISKSQLLSFIHRTSERGMYTSGRGSSAVGLTASVAKDPDTGQFILESGALVLSDNGICCIDEFDKMSDSTRSVLHEVMEQQTVSVAKAGIITTLNARCSILASCNPIESKYNPRKSIVENINLPPTLLSRFDVVCLMIDRCDEFYDRTIGDHIVSLYSEETQRKEYIDADLLKAYVREARRIVPRLTPESMKMLTQSYVDLRQMDNGKTITATTRQLESLIRLSEAHARMRFSNAVEAKDVREAVRIIRESLLMYAIDPSTGKVDMDMIITGRSTFKNRMLENLKDAILLMVKGKAEVSDVVKNTGAEEKMVLEALEELVLEGKMIASVQGSCYERISD DIP protein Q8SSE5 original sequence version: 75 q8sql8_enccu DNA replication licensing factor MCM7 MCM7 ECU09_1360 protein protein enccu Encephalitozoon cuniculi (strain GB-M1) Microsporidian parasite MNQVSELRRFETPIDYQADKDRLSKFLLFHEEDGELTYANRLREAGGSVEIDMEDIAVYDETGLVGRIEGNAMSYINLLYTVVDEILLEGGDVPMGEEPEDIFFYHRVSRLKERFPEKSALEVFPSFLLRRYSLVLKPRRNSRVYSVRGLKSMHIGSLVRVSGVVTKVSQVKPSIRVATYVCEGCGAETYQEVDGDVFDLLEECGSEKCRIRNVRGTLVLVTRGSKFIKHQTVYMQELTGDIPRGCIPRTLVAECYSSMAEKCRPGDVVVVGGVFMPKPYHGIKKLKAGLLADIYLHATNIEKIGAGAPKGNEEVKAYPVEQMVRSIAPEIFGMEDIKKILLLMLIGAPGRVREDGMKIRGDINVLLVGDPGIAKSQLLKTCVKIGRRGVYTTGKGSSGVGLTASVSKDPITGEMVLEGGALVLADGGICCIDELDKMNEVDRVSIHEVMEQQSVSISKAGINTSLNARCCVLGAANPVKGKYDTRQSIEHNIGLPCALLSRFDVVAILRDEPNLEKDESLANHITSIHLHEEPESIPYDKIRLIIDEAKRINPVLPSHLSGKLTDAYVKARKESPYVTPRYLLSLIRLSLAHCRLRLSTDVNEDDVNEALRLMEVTRIPVPRKRREEVSSKRAIYNLILSLAGEEGGRRCVKLAHLWEATKDKYSVSEVEDVISDFASCGIWIRNNEELVIFN DIP protein Q8SQL8 original sequence version: 69 q8srx5_enccu DNA replication licensing factor MCM6 ECU05_0780 protein protein enccu Encephalitozoon cuniculi (strain GB-M1) Microsporidian parasite MTEEVHDKFLEFLYNTPKVRAAAEQAIYRNEGVLLVDLRDVSAYSAELHADLLLNFSRNVERIDRSTAVYTMREFSTALEHTSFCNSHVIYKIRELKSNRLGQLLSFSGTVTRTTQVRPELVSGTFVCKICGSVIDNVFQEFKYTEPLTCPNHLCTNRRLWKLDIDKSKFLNWQRIHVQENTEEIPPGSLPRSMDVIVRNDLVEKIRAGDKVVMTGYLIVVPDVVQLMMPQSKSVPMQSGESDEIRKKRNINIKDLNHKLSFMCIHAGCSVEEDEEFTNEELATISEMRSTPDLYYKLSQSMFPSIHGHYSIKNAILLLLVGGVGKRAEGGTRLRGDINMLLVGDPGTAKSQFLKQASAFLPRSVYTSGKSSSAAGLTASVVKDGETGEFTIEAGALMLSDTGVCCIDEFDKMNVKDQVSIHEAMEQQTITISKAGINATLNARSSILAAANPIKGRYDKKKTLRQNINLSAPVMSRFDLYFVLIDDADPENDRNVATHVLNSHASVTDSGVLASYFTREQVKLYLRYARKKTPRMTAEAKEMLIKRYVGIRQDSLIHSNNYMMTVRHLESLIRLSEALAKVHDNDLVTKEYVEEAHRLVKSSVVEVKGEDIEIIPKTADEPGFMVSSKDYVRITNSFIYLIKTREPMERRELVEALLSESESSIESERALVDEQNKAENVLSFLIGREGVLFISDGKIHIHPSYDV 828FCF4CA5BFC844 q8srs4_enccu DNA replication licensing factor MCM5 ECU06_0340 protein protein enccu Encephalitozoon cuniculi (strain GB-M1) Microsporidian parasite MTWDEHRVTSIDLVTNDPVVNRETVKNEFIRFIELFKTTHKEYSHVLSSNINQDNFSLTVKLEHIYQYSDLLSRELSRRPERAIEWFEEAVADRYNVKGVQGFQLCLVSDGRCIPIREINASKTNKIVKIQGIVVSASSVITKPRTLFLVCRNCLSSKEVMDMIPRMCDKTECPPDPYIVIPEKSQVIDVQYVKIQEFFEDIPVGETPRHFSLVLEKKMVNRLIPGSKVVITGIYCMRMIRDSSLPIVKVVGLEDRSLKTSKMFTEEEEESFKKLSKADIYERISRSIAPSVYGHEDVKRALACMLFGGTRRVLEDKVTLRGDINVLLLGDPGMAKSQLLKFMELVSPVGVYTSGKGSSAAGLTASVIRDSGGEFYLEGGALVLADNGICCIDEFDKMDEHDRVAIHEAMEQQTISIAKAGITTMLNTRTSILAAANPVFGRYDDYKTPDENIEFGATILSRFDCIFILKDKHGPNDIILAKHVLSVHQNKAREDNECQNGLHDDQEEQISGSDRSPDIIPIHTIKRYVQYARSKVFPTLSEAASKQLSRYYVNTRKEVRQLEQSTLKRNAIPITVRQLEAIIRIGESLAKMELSQIVTEKHVEEAIRLFKVSTMNAVSQGHMLEGMVRPDVINHIEEICSRIRLLVPIGGSIRYSDLIRKLGGKDEWVCKKAIDYMCKQEKLVARDMGRVLIRQP DIP protein Q8SRS4 original sequence version: 75 q8srf0_enccu DNA replication licensing factor MCM3 ECU08_0290 protein protein enccu Encephalitozoon cuniculi (strain GB-M1) Microsporidian parasite MESEFIELFKSFIESYRTPQDKDIAEEIAKLPYEDHQRIVLNVNDMRLFDKGLARGVLEDPATLVPFIEDELSDIGGRKIFLGIKGSFGNYFLNPRTLSSVYIGKMVCVEGIVTSCSICRPKVMKSVHYNPSKNVFYSKEYRDATMVTKLPVTNSVYPTRDVDGTLLTTEFGLSEYFDYQTVVLQEMPEKAPPGQLPRSVEVILSFDLVDGLKPGDRAKIYGIYKSLCFGNQQFPSRFRTVVIANNIEKAEEEEVEVEPGVEFERLSQMKNMHHLIAPSIFGHDIVKRSIALLLVGGNEVVMRNGSKIRGDINILLVGDPSTAKSQLLRYVLNAAQLSIATTGKGSSGVGLTAAVVLDKDTGEKRLEAGAMVLADRGVVCIDEFDKMSDGDRVAIHEVMEQQTVTIAKAGIHTTLNARCSVLAAANPIWGQYKESRPPQDNVRLPESLLTRFDLIFVTLDKSNTDIDQLVSGHVLRMHMLTQGYEEEGMGVKQEVFRAYIRYCRQKKPVLSREAAGLIAREYTSLRQSKDRKEQIVSITPRMLETMIRLATANAKLRLSDVVEYDDAECAVNLVKDSLFQKIVKPAKRIKAAAPEEQDEFDLVDEKGTLKQRLEAAMIDEDKINFVSHALYDYSADPKNPRILEVQDFMKYLGTDVSVTEQEVEEILSRLAEKDLILFENGRIYLLN DIP protein Q8SRF0 original sequence version: 76 q8ss42_enccu DNA replication licensing factor MCM2 Minichromosome maintenance protein 2 ECU04_0850 protein protein enccu Encephalitozoon cuniculi (strain GB-M1) Microsporidian parasite MAKRRFEEDESMSEEERILDAGGVQELESEDMDEIRDDLYESEGMYEESEATEHSDIEDLISQEVKNVRPDERKGGNGDAEYDAEDILKASEVTKDFFEQDFFRAKVVRKFIRFFNEFGNKKYAKRIRQMCTENLESIEVSYLDIEEESIDLLRLLNQHAEMTIEVMDRALSDVVRMHFPNYHMIKPKVHSRVVDLPVCDSVRSLRNRHLGKLVRVSGVVTRRSGVFPLYSIVKFSCLKCRSVFGPFVASSFKPTHCFECQSKGPFTVNTSETVYKDFQKLTIQEIPGSVPPGSLPRSKEVLLFYDLIDCAKPGEEVEVTGVYKNNFNVSLNIRNGFPVFFTVIEASSVVKRAGKIEMTDDDVREIKKMGRHPEIKRIVINSIAPSVYGHAEVKRAIALAMLGGVARESTSHRIRGDINVLLLGDPGMAKSQFLRYVESTSHRAVLATGQGASSVGLTASVRKDPVVKEWTLEGGALVLADKGICLIDEFDKMNEHDRTSIHEAMEQQSISISKAGIVATLHARCSVIAAANPMRGRYNGSLTFAQNVNLSDPIISRFDILCVVKDAIDAGEDEKTAKFVIESHEGGEEKPDGFDPKRMMMGHELLRKYILYARTNVVPAFNDVDMEKISSLYLELRKESLPSGLPVTVRHVESIVRISEAFAKMRLSRVVSVEDIDEAISVVLDSFMGAQKYSMSKSLRKKFVKYFNKNNIDVLVFLLKEMFNEKMKAFRSQSVSVDEFERRISSFGFSIPSNFYSCGLFKDSGFRLDREARLILRSVY DIP protein Q8SS42 original sequence version: 74 mcm6-q8sql8_enccu-1 3 6 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 5 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 10 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association mcm6-q8sql8_enccu-2 4 6 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 5 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 10 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association mcm6-q8sql8_enccu-3 2 6 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 5 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 10 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association mcm6-q8sql8_enccu-4 1 6 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 5 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 10 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association