DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural and energetic basis of ALS-causing mutations in the atypical proline-tyrosine nuclear localization signal of the Fused in Sarcoma protein (FUS). Structural and energetic basis of ALS-causing mutations in the atypical proline-tyrosine nuclear localization signal of the Fused in Sarcoma protein (FUS). Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Zhang ZC., Chook YM. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Zhang ZC., Chook YM. DIP original experiment: DIP-251720X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural and energetic basis of ALS-causing mutations in the atypical proline-tyrosine nuclear localization signal of the Fused in Sarcoma protein (FUS). Structural and energetic basis of ALS-causing mutations in the atypical proline-tyrosine nuclear localization signal of the Fused in Sarcoma protein (FUS). Structural and energetic basis of ALS-causing mutations in the atypical proline-tyrosine nuclear localization signal of the Fused in Sarcoma protein (FUS). Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Zhang ZC., Chook YM. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhang ZC., Chook YM. DIP original experiment: DIP-251719X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural and energetic basis of ALS-causing mutations in the atypical proline-tyrosine nuclear localization signal of the Fused in Sarcoma protein (FUS). fus_human RNA-binding protein FUS FUS TLS Oncogene FUS Oncogene TLS Translocated in liposarcoma protein POMp75 75 kDa DNA-pairing protein protein protein human Homo sapiens Human MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQSTDTSGYGQSSYSSYGQSQNTGYGTQSTPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQPAPSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNSSSGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRGGRGRGGSGGGGGGGGGGYNRSSGGYEPRGRGGGRGGRGGMGGSDRGGFNKFGGPRDQGSRHDSEQDNSDNNTIFVQGLGENVTIESVADYFKQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWFDGKEFSGNPIKVSFATRRADFNRGGGNGRGGRGRGGPMGRGGYGGGGSGGGGRGGFPSGGGGGGGQQRAGDWKCPNPTCENMNFSWRNECNQCKAPKPDGPGGGPGGSHMGGNYGDDRRGGRGGYDRGGYRGRGGDRGGFRGGRGGGDRGGFGPGKMDSRGEHRQDRRERPY DIP protein P35637 original sequence version: 165 DIP protein P35637 original sequence version: 156 DIP protein P35637-1 original sequence version: 156 DIP protein P35637-1 original sequence version: 92 DIP protein P35637-1 original sequence version: 146 88C8E263B7905549 q92973-2 Transportin-1 Importin beta-2 KPNB2 Karyopherin beta-2 M9 region interaction protein MIP1 TRN TNPO1 protein protein human Homo sapiens Human MEYEWKPDEQGLQQILQLLKESQSPDTTIQRTVQQKLEQLNQYPDFNNYLIFVLTKLKSEDEPTRSLSGLILKNNVKAHFQNFPNGVTDFIKSECLNNIGDSSPLIRATVGILITTIASKGELQNWPDLLPKLCSLLDSEDYNTCEGAFGALQKICEDSAEILDSDVLDRPLNIMIPKFLQFFKHSSPKIRSHAVACVNQFIISRTQALMLHIDSFIENLFALAGDEEPEVRKNVCRALVMLLEVRMDRLLPHMHNIVEYMLQRTQDQDENVALEACEFWLTLAEQPICKDVLVRHLPKLIPVLVNGMKYSDIDIILLKGDVEEDETIPDSEQDIRPRFHRSRTVAQQHDEDGIEEEDDDDDEIDDDDTISDWNLRKCSAAALDVLANVYRDELLPHILPLLKELLFHHEWVVKESGILVLGAIAEGCMQGMIPYLPELIPHLIQCLSDKKALVRSITCWTLSRYAHWVVSQPPDTYLKPLMTELLKRILDSNKRVQEAACSAFATLEEEACTELVPYLAYILDTLVFAFSKYQHKNLLILYDAIGTLADSVGHHLNKPEYIQMLMPPLIQKWNMLKDEDKDLFPLLECLSSVATALQSGFLPYCEPVYQRCVNLVQKTLAQAMLNNAQPDQYEAPDKDFMIVALDLLSGLAEGLGGNIEQLVARSNILTLMYQCMQDKMPEVRQSSFALLGDLTKACFQHVKPCIADFMPILGTNLNPEFISVCNNATWAIGEISIQMGIEMQPYIPMVLHQLVEIINRPNTPKTLLENTAITIGRLGYVCPQEVAPMLQQFIRPWCTSLRNIRDNEEKDSAFRGICTMISVNPSGVIQDFIFFCDAVASWINPKDDLRDMFCKILHGFKNQVGDENWRRFSDQFPLPLKERLAAFYGV Contains a N-acetylmethionine at position 1. DIP protein Q92973-2 original sequence version: 135 4762DB4895472D10 fus-tnpo1-1 2 3 unspecified role unspecified role unspecified role unspecified role Region 498-526 Region 498-526 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction fus-tnpo1-2 1 3 unspecified role unspecified role unspecified role unspecified role Region 498-526 Region 498-526 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P35637:p.Tyr526Ala Y526A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y526A'. Wed May 02 19:14:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr526ala' Tue Jun 30 17:01:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr526Ala' P35637:p.Arg518Ala R518A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R518A'. Wed May 02 15:38:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg518ala' Tue Jun 30 17:25:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg518Ala' P35637:p.Pro525Ala P525A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain P A This feature has been corrected as a result of our quality control procedures. The original label was 'P525A'. Wed May 02 15:59:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro525ala' Tue Jun 30 20:19:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro525Ala' P35637:p.Arg522Ala R522A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R522A'. Wed May 02 19:00:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg522ala' Tue Jun 30 19:43:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg522Ala' P35637:p.[Arg514Ala;Arg518Ala;Arg521Ala;Arg522Ala] R514A/R518A/R521A/R522A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R514A/R518A/R521A/R522A'. Wed May 02 15:35:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg514ala,arg518ala,arg521ala,arg522ala' Tue Jun 30 16:38:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg514Ala;Arg518Ala;Arg521Ala;Arg522Ala]' P35637:p.[Pro525Ala;Tyr526Ala] P525A/Y526A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain P A This feature has been corrected as a result of our quality control procedures. The original label was 'P525A/Y526A'. Wed May 02 15:23:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro525ala,tyr526ala' Tue Jun 30 16:48:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro525Ala;Tyr526Ala]' P35637:p.[Pro508Ala;Gly509Ala;Lys510Ala;Met511Ala] P508A/G509A/K510A/M511A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain M A certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain G A certain certain sequence position certain certain certain sequence position certain P A This feature has been corrected as a result of our quality control procedures. The original label was 'P508A/G509A/K510A/M511A'. Wed May 02 17:18:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro508ala,gly509ala,lys510ala,met511ala' Tue Jun 30 16:00:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro508Ala;Gly509Ala;Lys510Ala;Met511Ala]' P35637:p.Pro525Leu P525L mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain P L This feature has been corrected as a result of our quality control procedures. The original label was 'P525L'. Wed May 02 20:03:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro525leu' Tue Jun 30 17:17:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro525Leu' P35637:p.His517Gln H517Q mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain H Q This feature has been corrected as a result of our quality control procedures. The original label was 'H517Q'. Wed May 02 17:55:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his517gln' Tue Jun 30 17:01:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His517Gln' P35637:p.Arg524Ser R524S mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R S This feature has been corrected as a result of our quality control procedures. The original label was 'R524S'. Wed May 02 14:34:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg524ser' Tue Jun 30 18:54:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg524Ser' P35637:p.Arg521Cys R521C mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R C This feature has been corrected as a result of our quality control procedures. The original label was 'R521C'. Wed May 02 20:14:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg521cys' Tue Jun 30 19:22:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg521Cys' P35637:p.[Arg514Ala;Arg518Ala] R514A/R518A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R514A/R518A'. Wed May 02 15:40:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg514ala,arg518ala' Tue Jun 30 17:26:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg514Ala;Arg518Ala]' P35637:p.[Arg521Ala;Arg522Ala] R521A/R522A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R521A/R522A'. Wed May 02 19:27:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg521ala,arg522ala' Tue Jun 30 16:14:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg521Ala;Arg522Ala]' ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction