DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Antisilencing role of the RNA-directed DNA methylation pathway and a histone acetyltransferase in Arabidopsis. Antisilencing role of the RNA-directed DNA methylation pathway and a histone acetyltransferase in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Li X., Qian W., Zhao Y., Wang C., Shen J., Zhu JK., Gong Z. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Li X., Qian W., Zhao Y., Wang C., Shen J., Zhu JK., Gong Z. DIP original experiment: DIP-251677X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Antisilencing role of the RNA-directed DNA methylation pathway and a histone acetyltransferase in Arabidopsis. Antisilencing role of the RNA-directed DNA methylation pathway and a histone acetyltransferase in Arabidopsis. Antisilencing role of the RNA-directed DNA methylation pathway and a histone acetyltransferase in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Li X., Qian W., Zhao Y., Wang C., Shen J., Zhu JK., Gong Z. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Li X., Qian W., Zhao Y., Wang C., Shen J., Zhu JK., Gong Z. DIP original experiment: DIP-251678X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Antisilencing role of the RNA-directed DNA methylation pathway and a histone acetyltransferase in Arabidopsis. h3k9-2 Histone H3 N-terminal Peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ARTKQTARKSTGGKAPRKQLA idm1_arath Increased DNA methylation 1 IDM1 ROS4 Histone H3 acetyltransferase IDM1 Protein ROS4 Repressor of silencing 4 K15M2.12 At3g14980 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLPGAEIEMLGGDCFEGSYEDHQIFREVFFGSDPGNTTKRCLVTGAINFECDSSKNVNSSLSSNSVVTSGYACPQGFEASASRDGSDFNTKAKRVKLSGNKHLDARDEKGSALHGFPTSDIARETIPLHLVESSNKGVSTSSYLLKHSIVKGREVYLGGIVSGKCKSLNLDKCDGKEFKAIASPVSQESFATRMISVGASTPHSEKACFPLQLNNGSKVSPNELIMSKTCLKIDPKEDPRPLLYKYVCKVLTAARWKIEKRERSAGRKHVDTFYISPEGRKFREFGSAWKALGGILLADRKLMDTGTKKWTGINDFWSDLSLTLLDIEENMKNLNLANTRALWWSALEPFVVVVFISKQVGSLRKGNKVEVARNSNPDKLKKEDTICLNLISGCPESVLTVSEGSHLVHDVDANQEIHSDLEVQTKISSQKVSSRLERQSIIGKEISGTHEQEASKGIVASKLIAEDMHESVMRKNLHRRSKKISDIKPASLDQHDSLDSNSLNSFEFQDKEMGNIHLVSKGSRDERLRNEKMNNSCCNSKKGRKKARKHYTQDDDLMGSTITRNKGKFSRSSQKKKTQKPKARTKKRNNRGGCRLLPRSSSNVENHFFQGNWSILGPRTVLSWLIATKVISRDEVIQLRDPDDDTVVKTGLVTKDGVVCTCCNKTVSLSEFKNHAGFNQNCPCLNLFMGSGKPFASCQLEAWSAEYKARRNGWRLEKASDDDPNDDSCGVCGDGGELICCDNCPSTFHQACLSMQVLPEGSWYCSSCTCWICSELVSDNAERSQDFKCSQCAHKYHGTCLQGISKRRKLFPETYFCGKNCEKVYNGLSSRVGIINPNADGLSWSILKCFQEDGMVHSARRLALKAECNSKLAVALSIMEESFLSMVDPRTGIDMIPHVLYNWGSTFARLDFDGFYTVVVEKDDVMISVASIRVHGVTIAEMPLVATCSKYRRQGMCRILVAAIEEMLMSLKVEKLVVAALPSLVETWTEGFGFKPMDDEERDALKRINLMVFPGTTLLKKTLYESTKPSTMKGVCLSKERNNPSNKEADLEPGLDKAGSPMSTQVESCDQMVPAGSDDEPSPGFPVPLGADQTEPTSETENPSRDSNANDRPNKTTVVSIGEEEEEECLQKDVSKLSEEGKETTRASSSSAALEEVSGLGLGVVNNVSDEMLLCVDEQLDSDSSQDSE DIP protein F4IXE7 original sequence version: 14 h4-2 Histone 4 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SGRGKGGKGLGKGGAKRHRKVLR h3(121)-idm1-1 1 3 unspecified role unspecified role bait bait K4me2 K4me2 n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk certain certain sequence position certain certain certain sequence position certain decreasing-ptm ptm decreasing an interaction decreasing-ptm K4me3 K4me3 n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain disrupting-ptm ptm disrupting an interaction disrupting-ptm Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal in vitro In vitro 4 unspecified role unspecified role prey prey PHD domain Region 723-769 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction h4(224)-idm1-1 2 5 unspecified role unspecified role bait bait Biotin-tag Biotin-tag biotin tag biotin tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal in vitro In vitro 4 unspecified role unspecified role prey prey PHD domain Region 723-769 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction