DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu An oxygen-regulated switch in the protein synthesis machinery. An oxygen-regulated switch in the protein synthesis machinery. Nature 2012 imex curation Uniacke J., Holterman CE., Lachance G., Franovic A., Jacob MD., Fabian MR., Payette J., Holcik M., Pause A., Lee S. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Uniacke J., Holterman CE., Lachance G., Franovic A., Jacob MD., Fabian MR., Payette J., Holcik M., Pause A., Lee S. DIP original experiment: DIP-250668X Nature 2012 IMEx An oxygen-regulated switch in the protein synthesis machinery. An oxygen-regulated switch in the protein synthesis machinery. An oxygen-regulated switch in the protein synthesis machinery. Nature 2012 imex curation Uniacke J., Holterman CE., Lachance G., Franovic A., Jacob MD., Fabian MR., Payette J., Holcik M., Pause A., Lee S. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Uniacke J., Holterman CE., Lachance G., Franovic A., Jacob MD., Fabian MR., Payette J., Holcik M., Pause A., Lee S. DIP original experiment: DIP-250664X Nature 2012 IMEx An oxygen-regulated switch in the protein synthesis machinery. An oxygen-regulated switch in the protein synthesis machinery. An oxygen-regulated switch in the protein synthesis machinery. Nature 2012 imex curation Uniacke J., Holterman CE., Lachance G., Franovic A., Jacob MD., Fabian MR., Payette J., Holcik M., Pause A., Lee S. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Uniacke J., Holterman CE., Lachance G., Franovic A., Jacob MD., Fabian MR., Payette J., Holcik M., Pause A., Lee S. DIP original experiment: DIP-250665X Nature 2012 IMEx An oxygen-regulated switch in the protein synthesis machinery. An oxygen-regulated switch in the protein synthesis machinery. An oxygen-regulated switch in the protein synthesis machinery. Nature 2012 imex curation Uniacke J., Holterman CE., Lachance G., Franovic A., Jacob MD., Fabian MR., Payette J., Holcik M., Pause A., Lee S. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Uniacke J., Holterman CE., Lachance G., Franovic A., Jacob MD., Fabian MR., Payette J., Holcik M., Pause A., Lee S. DIP original experiment: DIP-250663X Nature 2012 IMEx An oxygen-regulated switch in the protein synthesis machinery. An oxygen-regulated switch in the protein synthesis machinery. An oxygen-regulated switch in the protein synthesis machinery. Nature 2012 imex curation Uniacke J., Holterman CE., Lachance G., Franovic A., Jacob MD., Fabian MR., Payette J., Holcik M., Pause A., Lee S. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Uniacke J., Holterman CE., Lachance G., Franovic A., Jacob MD., Fabian MR., Payette J., Holcik M., Pause A., Lee S. DIP original experiment: DIP-250666X Nature 2012 IMEx An oxygen-regulated switch in the protein synthesis machinery. An oxygen-regulated switch in the protein synthesis machinery. An oxygen-regulated switch in the protein synthesis machinery. Nature 2012 imex curation Uniacke J., Holterman CE., Lachance G., Franovic A., Jacob MD., Fabian MR., Payette J., Holcik M., Pause A., Lee S. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Uniacke J., Holterman CE., Lachance G., Franovic A., Jacob MD., Fabian MR., Payette J., Holcik M., Pause A., Lee S. DIP original experiment: DIP-250662X Nature 2012 IMEx An oxygen-regulated switch in the protein synthesis machinery. An oxygen-regulated switch in the protein synthesis machinery. An oxygen-regulated switch in the protein synthesis machinery. Nature 2012 imex curation Uniacke J., Holterman CE., Lachance G., Franovic A., Jacob MD., Fabian MR., Payette J., Holcik M., Pause A., Lee S. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Uniacke J., Holterman CE., Lachance G., Franovic A., Jacob MD., Fabian MR., Payette J., Holcik M., Pause A., Lee S. DIP original experiment: DIP-250667X Nature 2012 IMEx An oxygen-regulated switch in the protein synthesis machinery. An oxygen-regulated switch in the protein synthesis machinery. An oxygen-regulated switch in the protein synthesis machinery. Nature 2012 imex curation Uniacke J., Holterman CE., Lachance G., Franovic A., Jacob MD., Fabian MR., Payette J., Holcik M., Pause A., Lee S. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Uniacke J., Holterman CE., Lachance G., Franovic A., Jacob MD., Fabian MR., Payette J., Holcik M., Pause A., Lee S. DIP original experiment: DIP-250661X Nature 2012 IMEx An oxygen-regulated switch in the protein synthesis machinery. if4e_human Eukaryotic translation initiation factor 4E mRNA cap-binding protein eIF-4F 25 kDa subunit EIF4E EIF4EL1 EIF4F protein protein human Homo sapiens Human MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV DIP protein P06730 original sequence version: 164 DIP protein P06730 original sequence version: 127 DIP protein P06730 original sequence version: 126 DIP protein P06730 original sequence version: 135 DIP protein P06730 original sequence version: 151 DIP protein P06730 original sequence version: 155 DIP protein P06730 original sequence version: 160 DIP protein P06730-1 original sequence version: 187 DIP protein P06730 original sequence version: 146 B869B8DE615E699D if4e2_human Eukaryotic translation initiation factor 4E type 2 EIF4E2 mRNA cap-binding protein type 3 Eukaryotic translation initiation factor 4E-like 3 Eukaryotic translation initiation factor 4E homologous protein mRNA cap-binding protein 4EHP eIF4E-like protein 4E-LP EIF4EL3 protein protein human Homo sapiens Human MNNKFDALKDDDSGDHDQNEENSTQKDGEKEKTERDKNQSSSKRKAVVPGPAEHPLQYNYTFWYSRRTPGRPTSSQSYEQNIKQIGTFASVEQFWRFYSHMVRPGDLTGHSDFHLFKEGIKPMWEDDANKNGGKWIIRLRKGLASRCWENLILAMLGEQFMVGEEICGAVVSVRFQEDIISIWNKTASDQATTARIRDTLRRVLNLPPNTIMEYKTHTDSIKMPGRLGPQRLLFQNLWKPRLNVP DIP protein O60573 original sequence version: 106 3D3075BFA48B3C12 epas1_human Endothelial PAS domain-containing protein 1 EPAS1 HIF2A MOP2 Member of PAS protein 2 Basic-helix-loop-helix-PAS protein MOP2 BHLHE73 PASD2 Hypoxia-inducible factor 2-alpha HIF-1-alpha-like factor PAS domain-containing protein 2 Class E basic helix-loop-helix protein 73 protein protein human Homo sapiens Human MTADKEKKRSSSERRKEKSRDAARCRRSKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESEAEADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLSLKNGSGFGKKSKDMSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVKVYNNCPPHNSLCGYKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRITELIGYHPEELLGRSAYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQPQCIMCVNYVLSEIEKNDVVFSMDQTESLFKPHLMAMNSIFDSSGKGAVSEKSNFLFTKLKEEPEELAQLAPTPGDAIISLDFGNQNFEESSAYGKAILPPSQPWATELRSHSTQSEAGSLPAFTVPQAAAPGSTTPSATSSSSSCSTPNSPEDYYTSLDNDLKIEVIEKLFAMDTEAKDQCSTQTDFNELDLETLAPYIPMDGEDFQLSPICPEERLLAENPQSTPQHCFSAMTNIFQPLAPVAPHSPFLLDKFQQQLESKKTEPEHRPMSSIFFDAGSKASLPPCCGQASTPLSSMGGRSNTQWPPDPPLHFGPTKWAVGDQRTEFLGAAPLGPPVSPPHVSTFKTRSAKGFGARGPDVLSPAMVALSNKLKLKRQLEYEEQAFQDLSGGDPPGGSTSHLMWKRMKNLRGGSCPLMPDKPLSANVPNDKFTQNPMRGLGHPLRHLPLPQPPSAISPGENSKSRFPPQCYATQYQDYSLSSAHKVSGMASRLLGPSFESYLLPELTRYDCEVNVPVLGSSTLLQGGDLLRALDQAT DIP protein Q99814 original sequence version: 115 DIP protein Q99814 original sequence version: 155 DIP protein Q99814 original sequence version: 157 DIP protein Q99814 original sequence version: 137 DIP protein Q99814 original sequence version: 135 4838989598234FC1 q9bwf3-1 RNA-binding protein 4 RBM4 RBM4A Lark homolog RNA-binding motif protein 4 RNA-binding motif protein 4a protein protein human Homo sapiens Human MVKLFIGNLPREATEQEIRSLFEQYGKVLECDIIKNYGFVHIEDKTAAEDAIRNLHHYKLHGVNINVEASKNKSKTSTKLHVGNISPTCTNKELRAKFEEYGPVIECDIVKDYAFVHMERAEDAVEAIRGLDNTEFQGKRMHVQLSTSRLRTAPGMGDQSGCYRCGKEGHWSKECPIDRSGRVADLTEQYNEQYGAVRTPYTMSYGDSLYYNNAYGALDAYYKRCRAARSYEAVAAAAASVYNYAEQTLSQLPQVQNTAMASHLTSTSLDPYDRHLLPTSGAAATAAAAAAAAAAVTAASTSYYGRDRSPLRRATAPVPTVGEGYGYGHESELSQASAAARNSLYDMARYEREQYADRARYSAF DIP protein Q9BWF3-1 original sequence version: 124 DIP protein Q9BWF3 original sequence version: 104 if4a1_human Eukaryotic initiation factor 4A-I EIF4A1 EIF4A DDX2A ATP-dependent RNA helicase eIF4A-1 protein protein human Homo sapiens Human MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI DIP protein P60842 original sequence version: 125 DIP protein P60842 original sequence version: 59 DIP protein P60842 original sequence version: 63 DIP protein P60842 original sequence version: 85 DIP protein P60842 original sequence version: 86 DIP protein P60842 original sequence version: 92 DIP protein P60842-1 original sequence version: 114 DIP protein P60842 original sequence version: 106 6EF89939F3045420 4ebp1_human Eukaryotic translation initiation factor 4E-binding protein 1 Phosphorylated heat- and acid-stable protein regulated by insulin 1 EIF4EBP1 protein protein human Homo sapiens Human MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI DIP protein Q13541 original sequence version: 96 DIP protein Q13541 original sequence version: 103 DIP protein Q13541 original sequence version: 120 DIP protein Q13541 original sequence version: 113 1682A6BA74132966 epas1-rbm4-1 8 12 unspecified role unspecified role prey prey human Homo sapiens Human 11 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association epas1-eif4e2-1 7 10 unspecified role unspecified role prey prey human Homo sapiens Human 11 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association rbm4-eif4a1-2 5 13 unspecified role unspecified role prey prey human Homo sapiens Human 10 unspecified role unspecified role prey prey human Homo sapiens Human 12 unspecified role unspecified role bait bait human Homo sapiens Human 11 unspecified role unspecified role prey prey human Homo sapiens Human association association epas1-rbm4-2 2 12 unspecified role unspecified role prey prey human Homo sapiens Human 11 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range N-terminal domain Region 2-290 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human physical association physical association eif4ebp1-eif4e-4 1 9 unspecified role unspecified role prey prey human Homo sapiens Human 14 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association epas1-rbm4-3 6 12 unspecified role unspecified role prey prey human Homo sapiens Human 11 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association epas1-eif4a1-1 3 13 unspecified role unspecified role prey prey human Homo sapiens Human 10 unspecified role unspecified role prey prey human Homo sapiens Human 12 unspecified role unspecified role prey prey human Homo sapiens Human 11 unspecified role unspecified role bait bait human Homo sapiens Human association association rbm4-epas1-1 4 12 unspecified role unspecified role bait bait human Homo sapiens Human 11 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association