DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Peptidomics approach to elucidate the proteolytic regulation of bioactive peptides. Peptidomics approach to elucidate the proteolytic regulation of bioactive peptides. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Kim YG., Lone AM., Nolte WM., Saghatelian A. Only protein-protein interactions in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Kim YG., Lone AM., Nolte WM., Saghatelian A. DIP original experiment: DIP-251655X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Peptidomics approach to elucidate the proteolytic regulation of bioactive peptides. Peptidomics approach to elucidate the proteolytic regulation of bioactive peptides. Peptidomics approach to elucidate the proteolytic regulation of bioactive peptides. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Kim YG., Lone AM., Nolte WM., Saghatelian A. Only protein-protein interactions in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Kim YG., Lone AM., Nolte WM., Saghatelian A. DIP original experiment: DIP-251656X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Peptidomics approach to elucidate the proteolytic regulation of bioactive peptides. p14735-1 Insulin-degrading enzyme IDE Abeta-degrading protease Insulin protease Insulysin protein protein human Homo sapiens Human MRYRLAWLLHPALPSTFRSVLGARLPPPERLCGFQKKTYSKMNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL DIP protein P14735-1 original sequence version: 138 DIP protein P14735 original sequence version: 108 DIP protein P14735-1 original sequence version: 149 DIP protein P14735 original sequence version: 134 ide_mouse Insulin-degrading enzyme Ide Insulin protease Insulysin protein protein mouse Mus musculus Mouse MRNGLVWLLHPALPGTLRSILGARPPPAKRLCGFPKQTYSTMSNPAIQRIEDQIVKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIPGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLLDASCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVREELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLRQLYKIVPIKDIRNLYVTFPIPDLQQYYKSNPGYYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGKLHYYPLNGVLTAEYLLEEFRPDLIDMVLDKLRPENVRVAIVSKSFEGKTDRTEQWYGTQYKQEAIPEDVIQKWQNADLNGKFKLPTKNEFIPTNFEILSLEKDATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKRYNDKQPILLKKITEKMATFEIDKKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGVMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKAIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNYDRDNIEVAYLKTLTKDDIIRFYQEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPSQNDINLSEAPPLPQPEVIHNMTEFKRGLPLFPLVKPHINFMAAKL DIP protein Q9JHR7 original sequence version: 94 calca_mouse Calcitonin gene-related peptide 1 Calca Calc Alpha-type CGRP Calcitonin gene-related peptide I protein protein mouse Mus musculus Mouse MGFLKFSPFLVVSILLLYQACSLQAVPLRSILESSPGMATLSEEEVRLLAALVQDYMQMKARELEQEEEQEAEGSSVTAQKRSCNTATCVTHRLAGLLSRSGGVVKDNFVPTNVGSEAFGRRRRDLQA DIP protein Q99JA0 original sequence version: 98 calca-ide-1 1 5 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 83-119 Region 83-119 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 4 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli protein cleavage protein cleavage calca-ide-2 2 3 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli 5 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 83-119 Region 83-119 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro protein cleavage protein cleavage