DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Ohto U., Fukase K., Miyake K., Shimizu T. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ohto U., Fukase K., Miyake K., Shimizu T. DIP original experiment: DIP-251644X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Ohto U., Fukase K., Miyake K., Shimizu T. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Ohto U., Fukase K., Miyake K., Shimizu T. DIP original experiment: DIP-251645X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Ohto U., Fukase K., Miyake K., Shimizu T. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly saxs x ray scattering saxs predetermined predetermined participant predetermined Ohto U., Fukase K., Miyake K., Shimizu T. DIP original experiment: DIP-251647X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Ohto U., Fukase K., Miyake K., Shimizu T. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ohto U., Fukase K., Miyake K., Shimizu T. DIP original experiment: DIP-251646X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Ohto U., Fukase K., Miyake K., Shimizu T. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly saxs x ray scattering saxs predetermined predetermined participant predetermined Ohto U., Fukase K., Miyake K., Shimizu T. DIP original experiment: DIP-251648X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of species-specific endotoxin sensing by innate immune receptor TLR4/MD-2. ly96_human Lymphocyte antigen 96 Protein MD-2 ESOP-1 LY96 ESOP1 MD2 protein protein human Homo sapiens Human MLPFLFFSTLFSSIFTEAQKQYWVCNSSDASISYTYCDKMQYPISINVNPCIELKRSKGLLHIFYIPRRDLKQLYFNLYITVNTMNLPKRKEVICRGSDDDYSFCRALKGETVNTTISFSFKGIKFSKGKYKCVVEAISGSPEEMLFCLEFVILHQPNSN DIP protein Q9Y6Y9 original sequence version: 79 DIP protein Q9Y6Y9 original sequence version: 86 DIP protein Q9Y6Y9 original sequence version: 109 DIP protein Q9Y6Y9 original sequence version: 112 0F92AFF583637C6B tlr4_mouse Toll-like receptor 4 Tlr4 Lps protein protein mouse Mus musculus Mouse MMPPWLLARTLIMALFFSCLTPGSLNPCIEVVPNITYQCMDQKLSKVPDDIPSSTKNIDLSFNPLKILKSYSFSNFSELQWLDLSRCEIETIEDKAWHGLHHLSNLILTGNPIQSFSPGSFSGLTSLENLVAVETKLASLESFPIGQLITLKKLNVAHNFIHSCKLPAYFSNLTNLVHVDLSYNYIQTITVNDLQFLRENPQVNLSLDMSLNPIDFIQDQAFQGIKLHELTLRGNFNSSNIMKTCLQNLAGLHVHRLILGEFKDERNLEIFEPSIMEGLCDVTIDEFRLTYTNDFSDDIVKFHCLANVSAMSLAGVSIKYLEDVPKHFKWQSLSIIRCQLKQFPTLDLPFLKSLTLTMNKGSISFKKVALPSLSYLDLSRNALSFSGCCSYSDLGTNSLRHLDLSFNGAIIMSANFMGLEELQHLDFQHSTLKRVTEFSAFLSLEKLLYLDISYTNTKIDFDGIFLGLTSLNTLKMAGNSFKDNTLSNVFANTTNLTFLDLSKCQLEQISWGVFDTLHRLQLLNMSHNNLLFLDSSHYNQLYSLSTLDCSFNRIETSKGILQHFPKSLAFFNLTNNSVACICEHQKFLQWVKEQKQFLVNVEQMTCATPVEMNTSLVLDFNNSTCYMYKTIISVSVVSVIVVSTVAFLIYHFYFHLILIAGCKKYSRGESIYDAFVIYSSQNEDWVRNELVKNLEEGVPRFHLCLHYRDFIPGVAIAANIIQEGFHKSRKVIVVVSRHFIQSRWCIFEYEIAQTWQFLSSRSGIIFIVLEKVEKSLLRQQVELYRLLSRNTYLEWEDNPLGRHIFWRRLKNALLDGKASNPEQTAEEEQETATWT DIP protein Q9QUK6 original sequence version: 103 DIP protein Q9QUK6 original sequence version: 118 DIP protein Q9QUK6 original sequence version: 71 9C83B59F9A220C17 ly96_mouse Lymphocyte antigen 96 Protein MD-2 ESOP-1 Ly96 Esop1 Md2 protein protein mouse Mus musculus Mouse MLPFILFSTLLSPILTESEKQQWFCNSSDAIISYSYCDHLKFPISISSEPCIRLRGTNGFVHVEFIPRGNLKYLYFNLFISVNSIELPKRKEVLCHGHDDDYSFCRALKGETVNTSIPFSFEGILFPKGHYRCVAEAIAGDTEEKLFCLNFTIIHRRDVN DIP protein Q9JHF9 original sequence version: 80 DIP protein Q9JHF9 original sequence version: 91 E224D17E5D5429E2 o00206-1 Toll-like receptor 4 TLR4 hToll protein protein human Homo sapiens Human MMSASRLAGTLIPAMAFLSCVRPESWEPCVEVVPNITYQCMELNFYKIPDNLPFSTKNLDLSFNPLRHLGSYSFFSFPELQVLDLSRCEIQTIEDGAYQSLSHLSTLILTGNPIQSLALGAFSGLSSLQKLVAVETNLASLENFPIGHLKTLKELNVAHNLIQSFKLPEYFSNLTNLEHLDLSSNKIQSIYCTDLRVLHQMPLLNLSLDLSLNPMNFIQPGAFKEIRLHKLTLRNNFDSLNVMKTCIQGLAGLEVHRLVLGEFRNEGNLEKFDKSALEGLCNLTIEEFRLAYLDYYLDDIIDLFNCLTNVSSFSLVSVTIERVKDFSYNFGWQHLELVNCKFGQFPTLKLKSLKRLTFTSNKGGNAFSEVDLPSLEFLDLSRNGLSFKGCCSQSDFGTTSLKYLDLSFNGVITMSSNFLGLEQLEHLDFQHSNLKQMSEFSVFLSLRNLIYLDISHTHTRVAFNGIFNGLSSLEVLKMAGNSFQENFLPDIFTELRNLTFLDLSQCQLEQLSPTAFNSLSSLQVLNMSHNNFFSLDTFPYKCLNSLQVLDYSLNHIMTSKKQELQHFPSSLAFLNLTQNDFACTCEHQSFLQWIKDQRQLLVEVERMECATPSDKQGMPVLSLNITCQMNKTIIGVSVLSVLVVSVVAVLVYKFYFHLMLLAGCIKYGRGENIYDAFVIYSSQDEDWVRNELVKNLEEGVPPFQLCLHYRDFIPGVAIAANIIHEGFHKSRKVIVVVSQHFIQSRWCIFEYEIAQTWQFLSSRAGIIFIVLQKVEKTLLRQQVELYRLLSRNTYLEWEDSVLGRHIFWRRLRKALLDGKSWNPEGTVGTGCNWQEATSI DIP protein O00206-1 original sequence version: 86 DIP protein O00206-1 original sequence version: 91 DIP protein O00206 original sequence version: 135 DIP protein O00206 original sequence version: 138 ly96-tlr4-12 4 8 unspecified role unspecified role unspecified role unspecified role Region 19-160 Region 19-160 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role unspecified role unspecified role Extracellular domain Region 22-627 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction ly96-tlr4-13 1 8 unspecified role unspecified role unspecified role unspecified role Region 19-160 Region 19-160 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role unspecified role unspecified role Extracellular domain Region 22-627 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction ly96-tlr4-14 5 6 unspecified role unspecified role unspecified role unspecified role Region 19-160 Region 19-160 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 9 unspecified role unspecified role unspecified role unspecified role Extracellular domain Region 23-629 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction ly96-tlr4-15 3 8 unspecified role unspecified role unspecified role unspecified role Region 19-160 Region 19-160 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role unspecified role unspecified role Extracellular domain Region 22-627 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction ly96-tlr4-16 2 8 unspecified role unspecified role unspecified role unspecified role Region 19-160 Region 19-160 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role unspecified role unspecified role Extracellular domain Region 22-627 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction