DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structure (London, England : 1993) 2012 imex curation Ma X., Lin WY., Chen Y., Stawicki S., Mukhyala K., Wu Y., Martin F., Bazan JF., Starovasnik MA. Only protein-protein interactions in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Ma X., Lin WY., Chen Y., Stawicki S., Mukhyala K., Wu Y., Martin F., Bazan JF., Starovasnik MA. DIP original experiment: DIP-249317X Structure (London, England : 1993) 2012 IMEx Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structure (London, England : 1993) 2012 imex curation Ma X., Lin WY., Chen Y., Stawicki S., Mukhyala K., Wu Y., Martin F., Bazan JF., Starovasnik MA. Only protein-protein interactions trini Trichoplusia ni (Cabbage looper) Cabbage looper molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Ma X., Lin WY., Chen Y., Stawicki S., Mukhyala K., Wu Y., Martin F., Bazan JF., Starovasnik MA. DIP original experiment: DIP-249315X Structure (London, England : 1993) 2012 IMEx Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structure (London, England : 1993) 2012 imex curation Ma X., Lin WY., Chen Y., Stawicki S., Mukhyala K., Wu Y., Martin F., Bazan JF., Starovasnik MA. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Ma X., Lin WY., Chen Y., Stawicki S., Mukhyala K., Wu Y., Martin F., Bazan JF., Starovasnik MA. DIP original experiment: DIP-249314X Structure (London, England : 1993) 2012 IMEx Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structure (London, England : 1993) 2012 imex curation Ma X., Lin WY., Chen Y., Stawicki S., Mukhyala K., Wu Y., Martin F., Bazan JF., Starovasnik MA. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ma X., Lin WY., Chen Y., Stawicki S., Mukhyala K., Wu Y., Martin F., Bazan JF., Starovasnik MA. DIP original experiment: DIP-249312X Structure (London, England : 1993) 2012 IMEx Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structure (London, England : 1993) 2012 imex curation Ma X., Lin WY., Chen Y., Stawicki S., Mukhyala K., Wu Y., Martin F., Bazan JF., Starovasnik MA. Only protein-protein interactions in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Ma X., Lin WY., Chen Y., Stawicki S., Mukhyala K., Wu Y., Martin F., Bazan JF., Starovasnik MA. DIP original experiment: DIP-249318X Structure (London, England : 1993) 2012 IMEx Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structure (London, England : 1993) 2012 imex curation Ma X., Lin WY., Chen Y., Stawicki S., Mukhyala K., Wu Y., Martin F., Bazan JF., Starovasnik MA. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Ma X., Lin WY., Chen Y., Stawicki S., Mukhyala K., Wu Y., Martin F., Bazan JF., Starovasnik MA. DIP original experiment: DIP-249313X Structure (London, England : 1993) 2012 IMEx Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structure (London, England : 1993) 2012 imex curation Ma X., Lin WY., Chen Y., Stawicki S., Mukhyala K., Wu Y., Martin F., Bazan JF., Starovasnik MA. Only protein-protein interactions trini Trichoplusia ni (Cabbage looper) Cabbage looper x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ma X., Lin WY., Chen Y., Stawicki S., Mukhyala K., Wu Y., Martin F., Bazan JF., Starovasnik MA. DIP original experiment: DIP-249316X Structure (London, England : 1993) 2012 IMEx Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structure (London, England : 1993) 2012 imex curation Ma X., Lin WY., Chen Y., Stawicki S., Mukhyala K., Wu Y., Martin F., Bazan JF., Starovasnik MA. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Ma X., Lin WY., Chen Y., Stawicki S., Mukhyala K., Wu Y., Martin F., Bazan JF., Starovasnik MA. DIP original experiment: DIP-249319X Structure (London, England : 1993) 2012 IMEx Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. q6zmj4-1 Interleukin-34 IL34 C16orf77 protein protein human Homo sapiens Human MPRGFTWLRYLGIFLGVALGNEPLEMWPLTQNEECTVTGFLRDKLQYRSRLQYMKHYFPINYKISVPYEGVFRIANVTRLQRAQVSERELRYLWVLVSLSATESVQDVLLEGHPSWKYLQEVETLLLNVQQGLTDVEVSPKVESVLSLLNAPGPNLKLVRPKALLDNCFRVMELLYCSCCKQSSVLNWQDCEVPSPQSCSPEPSLQYAATQLYPPPPWSPSSPPHSTGSVRPVRAQGEGLLP DIP protein Q6ZMJ4-1 original sequence version: 66 DIP protein Q6ZMJ4-1 original sequence version: 57 csf1_human Macrophage colony-stimulating factor 1 CSF1 Lanimostim protein protein human Homo sapiens Human MTAPGAAGRCPPTTWLGSLLLLVCLLASRSITEEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDKACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQDVVTKPDCNCLYPKAIPSSDPASVSPHQPLAPSMAPVAGLTWEDSEGTEGSSLLPGEQPLHTVDPGSAKQRPPRSTCQSFEPPETPVVKDSTIGGSPQPRPSVGAFNPGMEDILDSAMGTNWVPEEASGEASEIPVPQGTELSPSRPGGGSMQTEPARPSNFLSASSPLPASAKGQQPADVTGTALPRVGPVRPTGQDWNHTPQKTDHPSALLRDPPEPGSPRISSLRPQGLSNPSTLSAQPQLSRSHSSGSVLPLGELEGRRSTRDRRSPAEPEGGPASEGAARPLPRFNSVPLTDTGHERQSEGSFSPQLQESVFHLLVPSVILVLLAVGGLLFYRWRRRSHQEPQRADSPLEQPEGSPLTQDDRQVELPV DIP protein P09603 original sequence version: 166 DIP protein P09603-1 original sequence version: 127 DIP protein P09603 original sequence version: 130 DIP protein P09603 original sequence version: 139 DIP protein P09603 original sequence version: 135 656B0894F9255AED csf1r_human Macrophage colony-stimulating factor 1 receptor CSF1R FMS Proto-oncogene c-Fms CSF-1 receptor protein protein human Homo sapiens Human MGPGVLLLLLVATAWHGQGIPVIEPSVPELVVKPGATVTLRCVGNGSVEWDGPPSPHWTLYSDGSSSILSTNNATFQNTGTYRCTEPGDPLGGSAAIHLYVKDPARPWNVLAQEVVVFEDQDALLPCLLTDPVLEAGVSLVRVRGRPLMRHTNYSFSPWHGFTIHRAKFIQSQDYQCSALMGGRKVMSISIRLKVQKVIPGPPALTLVPAELVRIRGEAAQIVCSASSVDVNFDVFLQHNNTKLAIPQQSDFHNNRYQKVLTLNLDQVDFQHAGNYSCVASNVQGKHSTSMFFRVVESAYLNLSSEQNLIQEVTVGEGLNLKVMVEAYPGLQGFNWTYLGPFSDHQPEPKLANATTKDTYRHTFTLSLPRLKPSEAGRYSFLARNPGGWRALTFELTLRYPPEVSVIWTFINGSGTLLCAASGYPQPNVTWLQCSGHTDRCDEAQVLQVWDDPYPEVLSQEPFHKVTVQSLLTVETLEHNQTYECRAHNSVGSGSWAFIPISAGAHTHPPDEFLFTPVVVACMSIMALLLLLLLLLLYKYKQKPKYQVRWKIIESYEGNSYTFIDPTQLPYNEKWEFPRNNLQFGKTLGAGAFGKVVEATAFGLGKEDAVLKVAVKMLKSTAHADEKEALMSELKIMSHLGQHENIVNLLGACTHGGPVLVITEYCCYGDLLNFLRRKAEAMLGPSLSPGQDPEGGVDYKNIHLEKKYVRRDSGFSSQGVDTYVEMRPVSTSSNDSFSEQDLDKEDGRPLELRDLLHFSSQVAQGMAFLASKNCIHRDVAARNVLLTNGHVAKIGDFGLARDIMNDSNYIVKGNARLPVKWMAPESIFDCVYTVQSDVWSYGILLWEIFSLGLNPYPGILVNSKFYKLVKDGYQMAQPAFAPKNIYSIMQACWALEPTHRPTFQQICSFLQEQAQEDRRERDYTNLPSSSRSGGSGSSSSELEEESSSEHLTCCEQGDIAQPLLQPNNYQFC DIP protein P07333 original sequence version: 159 DIP protein P07333-1 original sequence version: 185 DIP protein P07333 original sequence version: 145 DIP protein P07333 original sequence version: 148 DIP protein P07333 original sequence version: 153 A8D99BE237573FE8 csf1r-il34-6 3 11 unspecified role unspecified role unspecified role unspecified role Region 20-299 Region 20-299 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 9 unspecified role unspecified role unspecified role unspecified role Region 21-193 Region 21-193 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction il34-csf1r-1 1 11 unspecified role unspecified role prey prey Region 20-299 Region 20-299 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 9 unspecified role unspecified role bait bait Region 21-193 Region 21-193 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction csf1r-il34-7 4 11 unspecified role unspecified role unspecified role unspecified role Region 20-299 Region 20-299 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 9 unspecified role unspecified role unspecified role unspecified role Region 21-193 Region 21-193 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction csf1-csf1r-18 8 10 unspecified role unspecified role unspecified role unspecified role Region 33-181 Region 33-181 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Region 20-299 Region 20-299 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction il34-1 2 9 unspecified role unspecified role unspecified role unspecified role Region 21-193 Region 21-193 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction csf1r-il34-8 6 11 unspecified role unspecified role unspecified role unspecified role Region 20-299 Region 20-299 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 9 unspecified role unspecified role unspecified role unspecified role Region 21-193 Region 21-193 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction csf1-csf1r-19 5 10 unspecified role unspecified role bait bait Region 33-181 Region 33-181 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 11 unspecified role unspecified role prey prey Region 20-299 Region 20-299 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction il34-2 7 9 unspecified role unspecified role unspecified role unspecified role Region 21-193 Region 21-193 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction