DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Discovery of an archetypal protein transport system in bacterial outer membranes. Discovery of an archetypal protein transport system in bacterial outer membranes. Nature structural & molecular biology 2012 imex curation Selkrig J., Mosbahi K., Webb CT., Belousoff MJ., Perry AJ., Wells TJ., Morris F., Leyton DL., Totsika M., Phan MD., Celik N., Kelly M., Oates C., Hartland EL., Robins-Browne RM., Ramarathinam SH., Purcell AW., Schembri MA., Strugnell RA., Henderson IR., Walker D., Lithgow T. Only protein-protein interactions Citrobacter freundii biotype 4280 Citrobacter rodentium (strain ICC168) Citrobacter freundii biotype 4280 anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Selkrig J., Mosbahi K., Webb CT., Belousoff MJ., Perry AJ., Wells TJ., Morris F., Leyton DL., Totsika M., Phan MD., Celik N., Kelly M., Oates C., Hartland EL., Robins-Browne RM., Ramarathinam SH., Purcell AW., Schembri MA., Strugnell RA., Henderson IR., Walker D., Lithgow T. DIP original experiment: DIP-250931X Nature structural & molecular biology 2012 IMEx Discovery of an archetypal protein transport system in bacterial outer membranes. Discovery of an archetypal protein transport system in bacterial outer membranes. Discovery of an archetypal protein transport system in bacterial outer membranes. Nature structural & molecular biology 2012 imex curation Selkrig J., Mosbahi K., Webb CT., Belousoff MJ., Perry AJ., Wells TJ., Morris F., Leyton DL., Totsika M., Phan MD., Celik N., Kelly M., Oates C., Hartland EL., Robins-Browne RM., Ramarathinam SH., Purcell AW., Schembri MA., Strugnell RA., Henderson IR., Walker D., Lithgow T. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Selkrig J., Mosbahi K., Webb CT., Belousoff MJ., Perry AJ., Wells TJ., Morris F., Leyton DL., Totsika M., Phan MD., Celik N., Kelly M., Oates C., Hartland EL., Robins-Browne RM., Ramarathinam SH., Purcell AW., Schembri MA., Strugnell RA., Henderson IR., Walker D., Lithgow T. DIP original experiment: DIP-250929X Nature structural & molecular biology 2012 IMEx Discovery of an archetypal protein transport system in bacterial outer membranes. Discovery of an archetypal protein transport system in bacterial outer membranes. Discovery of an archetypal protein transport system in bacterial outer membranes. Nature structural & molecular biology 2012 imex curation Selkrig J., Mosbahi K., Webb CT., Belousoff MJ., Perry AJ., Wells TJ., Morris F., Leyton DL., Totsika M., Phan MD., Celik N., Kelly M., Oates C., Hartland EL., Robins-Browne RM., Ramarathinam SH., Purcell AW., Schembri MA., Strugnell RA., Henderson IR., Walker D., Lithgow T. Only protein-protein interactions ecolx Escherichia coli bn-page blue native page bn-page western blot western blot Immuno blot Immuno blot Selkrig J., Mosbahi K., Webb CT., Belousoff MJ., Perry AJ., Wells TJ., Morris F., Leyton DL., Totsika M., Phan MD., Celik N., Kelly M., Oates C., Hartland EL., Robins-Browne RM., Ramarathinam SH., Purcell AW., Schembri MA., Strugnell RA., Henderson IR., Walker D., Lithgow T. DIP original experiment: DIP-250930X Nature structural & molecular biology 2012 IMEx Discovery of an archetypal protein transport system in bacterial outer membranes. tama_ecoli Translocation and assembly module subunit TamA tamA Autotransporter assembly factor TamA yftM ytfM JW4179 b4220 protein protein ecoli Escherichia coli (strain K12) MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLSTIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGVPVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFDSEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEYESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYSTDVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGFKRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMISRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGTLGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQYNVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHGLQFYIGLGPEL DIP protein P0ADE4 original sequence version: 56 253D1D5BEA744D25 tamb_citri Translocation and assembly module subunit TamB tamB Autotransporter assembly factor TamB ROD_32821 protein protein Citrobacter freundii biotype 4280 Citrobacter rodentium (strain ICC168) Citrobacter freundii biotype 4280 MSLWKKISLGVLIFILLLLATVGFLVGTTTGLHLVFSAANRWVPGLEIGQVTGGWRDLSLKNIRYDQPGVAVNAGEVHLAVGLECLWKSSLCVNDLSLKDINVVIDSKKMPPGEQVEEEEESGPLNLSTPYPVTLSRVALENINVKIDDTTVSVMDFTSGLNWQEKNLTLKPTALQGLLIALPKVAEVAQEEVVEPKIQNPQPDEKPLGETLQDLFSKPVLPEMTDVHLPLNLNIEEFKGEQLRLTGDTDLTVFSLLLKVSSIDGNMKLDALDIDSSQGAVNATGTAQLANNWPVDITLNSTLNVEPLKGEKIKLKVGGALREQLEVGVNLSGPLDVNLRAQARLAEAGLPLNLEVVSEQISWPFTGDRQFQADNTRLKLTGKMTDYTLSMRTAVKGQDVPPATITLDAKGNEQQINLDKLTVAALEGKTELKALVDWRQAISWRGELTLDGINTAKEVPDWPSKLNGLIKTRGSLYGGSWQMEVPELKLTGNVKQNKVNVNGSLKGNSYMQWTIPGLHLVLGPNSADVKGELGVKDLNLDATIDAPGLDNALPGLGGTAKGLVKVRGTVDAPQLLADITARGLRWQELSIAQVRVDGDIKSTDQIAGKLDVRVERISQPDVNINLVTLHAKGSEKQHELQLRIQGEPVSGQLDLAGSFDREEMRWKGTLSNTRFRTPVGPWSQTRAIALDYRGQEQKISIGPHCWTNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLKPFMPETTQASGVFSGKADVAWDTTKEGLPQGNVTLSGRSVKVTQTVNDAPLPLAFDTLNVSADLHDNRAELGWQIRLSNNGQLDGQVQVTDPQGRRNLGGNVSIRNLNLAMVNPIFARGEKAAGLLNANLRLGGDVQSPQMFGQLQLNGVDIDGNFMPFDMQPSQLAMNFNGTRSTLTGVVRTQQGEINLSGDADWSQIENWRARIAAKGSRVRITVPPMVRLDVSPDVVFEATPSLFTLDGRVDVPWARIVVHELPESAVGVSSDEVMLNNQLQPEEPQTAAIPINSNLIVHVGNNVRMDAFGLRARLTGDLKVAQDKQGLGLNGQINIPEGRFHAYGQDLLVRKGELLFSGPPDQPILNIEAIRNPEATEDDVIAGVRVTGSADEPKAEIFSDPAMSQQEALSYLLRGQGLDSNQSDSAAMTSMLIGLGVAQSGQVVGKIGETFGVSNLALDTQGVGDSSQVVVSGYVLPGLQVKYGVGIFDSLATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF DIP protein D2TN57 original sequence version: 12 tama_citri Translocation and assembly module subunit TamA tamA Autotransporter assembly factor TamA ROD_32811 protein protein Citrobacter freundii biotype 4280 Citrobacter rodentium (strain ICC168) Citrobacter freundii biotype 4280 MPHIRQLCWVSLMCLSSSAFAANVRLQVEGLSGELERNVRAQLSTIQSDEVTPDRRFRARVDDAIREGLKALGYYEPTIDFDLRPPPAKGRQVLLARVSPGEPVRIGGTGVILRGGARTDRDYLDLLKTRPAIGTVLNHGDYDNFKKSLTSVALRKGYFDSEFNKSQLGVALDRHQAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEYESKDLGELNRRLSATGWFNSVVVAPEFDKARETKVLPLKGVVSPRTENTVETGVGYSTDVGPRVKATWKKPWMNSYGHSLTTSASISAPEQVLDFSYKIPLLKNPLEQYYLVQGGFKRTDLNDTEQDSTTLALSRYWDLSSGWQRAINLRWSLDHFTQGEVTNTTMLLYPGVMISRTRSRGGLMPTWGDSQRYSVDYSNTAWGSDVDFSVIQAQNVWIRTLYDRHRFVMRGNLGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSISPKDSNGDLKGASKLATGSLEYQYNVTGKWWGAVFVDSGEAVSDIRRSDFKTGAGVGVRWQSPVGPIKLDFAAPIGDKDEHGLQFYIGLGPEL DIP protein D2TN56 original sequence version: 13 tamb_ecoli Translocation and assembly module subunit TamB tamB Autotransporter assembly factor TamB ytfN ytfO JW4180 b4221 protein protein ecoli Escherichia coli (strain K12) MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVPGLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQVNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSGLNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSRPVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDSSQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKMGGALREQLEIGVNLSGPVDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMRTAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTLNGINTAKEFPDWPSKLNGLIKTRGSLYGGTWQMDVPELKLTGNVKQNKVNVDGTLKGNSYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKVRGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNINLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPWSLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLKPFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTLNLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIFTRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMRSTLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFEATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNLIVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGELLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSYLLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQVVVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF DIP protein P39321 original sequence version: 79 CDBB43F0F8C81DB1 tama-tamb-1 2 4 unspecified role unspecified role unspecified role unspecified role Region 22-293 Region 22-293 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 1180-1259 Region 1180-1259 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 1253-1259 Region 1253-1259 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction Fig. 2G tamb-tama-1 1 6 unspecified role unspecified role prey prey Citrobacter freundii biotype 4280 Citrobacter rodentium (strain ICC168) Citrobacter freundii biotype 4280 5 unspecified role unspecified role bait bait Citrobacter freundii biotype 4280 Citrobacter rodentium (strain ICC168) Citrobacter freundii biotype 4280 physical association physical association tama-tamb-2 3 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association